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Human dental pulp cell apoptosis: Immunohistochemical study after applying orthodontic traction

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dc.contributor.author Leone A.
dc.contributor.author Angelova Volponi A.
dc.contributor.author Campanella C.
dc.contributor.author Guarnotta C.
dc.contributor.author Abdallah Hajj Hussein I.
dc.contributor.author Cappello F.
dc.contributor.author Gerbino A.
dc.contributor.author Jurjus A.
dc.contributor.editor
dc.date Oct-2012
dc.date.accessioned 2017-09-07T06:59:58Z
dc.date.available 2017-09-07T06:59:58Z
dc.date.issued 2012
dc.identifier
dc.identifier.isbn
dc.identifier.issn
dc.identifier.uri http://hdl.handle.net/10938/11223
dc.description.abstract The aim of this study was to compare human dental pulp stress and programmed cell death after 3 and 6 months of orthodontic treatments by assessing the degree of apoptosis and related proteins. Human dental pulps were collected from twenty young patients orthodontically treated by Straight Wire technique. Samples were fixed, paraffin-embedded and processed for histology and immunohistochemistry using anti-heat shock protein 60 kDa (Hsp60), -caspase 3, -caspase 9, and -PCNA antibodies, as well as TUNEL reactions. Moreover, we performed immunoprecipitation for Hsp60 and caspase 3, and for Hsp60 and caspase 9, from paraffin extracted tissues. Increased levels of both caspases and Hsp60 occurred in 6-months treated samples; at the same time, we found increased levels of proliferating cell nuclear antigen and terminal deoxynucleotidyl transferase dUTP nick end labeling positive cells. Immunoprecipitation showed that Hsp60 forms a complex with both Pro-caspase 3 and Caspase 3, and this may accelerate Pro-caspase 3 activation, especially in the 6-months treated group. On the contrary, no complex between Hsp60 and Pro-caspase 9 was detected. The orthodontic tractions may be a cause of stress, apoptosis and proliferation in pulp tissue. These results suggest the need of further studies about the effects of long term orthodontic treatments on the dental pulp. Copyright © by BIOLIFE, s.a.s.
dc.format.extent
dc.format.extent Pages: (713-720)
dc.language English
dc.publisher SILVA MARINA (TE)
dc.relation.ispartof Publication Name: Journal of Biological Regulators and Homeostatic Agents; Publication Year: 2012; Volume: 26; no. 4; Pages: (713-720);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other
dc.title Human dental pulp cell apoptosis: Immunohistochemical study after applying orthodontic traction
dc.type Article
dc.contributor.affiliation Leone, A., Department of Experimental Biomedicine and Neuroscience, Section of Histology and Embryology, University of Palermo, Italy
dc.contributor.affiliation Angelova Volponi, A., Department of Craniofacial Development and Stem Cell Biology, Dental Institute, King's College, London, United Kingdom
dc.contributor.affiliation Campanella, C., Department of Experimental Biomedicine and Neuroscience, University of Palermo, Italy
dc.contributor.affiliation Guarnotta, C., Department of Science and Health Promotion, University of Palermo, Italy
dc.contributor.affiliation Abdallah Hajj Hussein, I., Department of Anatomy, Cell Biology, and Physiology, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Cappello, F., Department of Experimental Biomedicine and Neuroscience, University of Palermo, Italy
dc.contributor.affiliation Gerbino, A., Department of Experimental Biomedicine and Neuroscience, Section of Histology and Embryology, University of Palermo, Italy
dc.contributor.affiliation Jurjus, A., Department of Anatomy, Cell Biology, and Physiology, American University of Beirut, Beirut, Lebanon
dc.contributor.authorAddress Leone, A.; BioNec. Facoltà di Medicina e Chirurgia, Sezione di Istologia ed Embriologia Generale, Università di Palermo, Via Del Vespro, 127, Palermo, Italy; email: angelo.leone@unipa.it
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Anatomy, Cell Biology and Physiological Sciences;
dc.contributor.authorDepartment Anatomy, Cell Biology and Physiological Sciences
dc.contributor.authorDivision
dc.contributor.authorEmail angelo.leone@unipa.it
dc.contributor.authorFaculty Faculty of Medicine
dc.contributor.authorInitials Leone, A
dc.contributor.authorInitials Volponi, AA
dc.contributor.authorInitials Campanella, C
dc.contributor.authorInitials Guarnotta, C
dc.contributor.authorInitials Hussein, IAH
dc.contributor.authorInitials Cappello, F
dc.contributor.authorInitials Gerbino, A
dc.contributor.authorInitials Jurjus, A
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Leone, A (reprint author), Univ Palermo, BioNec Fac Med and Chirurg, Sez Istol and Embriol Gen, Via Del Vespro 127, Palermo, Italy.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut Medical Center
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dc.description.citedCount
dc.description.citedTotWOSCount 0
dc.description.citedWOSCount 0
dc.format.extentCount 8
dc.identifier.articleNo
dc.identifier.coden JBRAE
dc.identifier.pubmedID 23241121
dc.identifier.scopusID 84876503885
dc.identifier.url
dc.publisher.address VIA S STEFANO 39 BIS, 64029 SILVA MARINA (TE), ITALY
dc.relation.ispartofConference
dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr J. Biol. Regul. Homeost. Agents
dc.relation.ispartOfIssue 4
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Journal of Biological Regulators and Homeostatic Agents
dc.relation.ispartofPubTitleAbbr J. Biol. Regul. Homeostatic Agents
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 26
dc.source.ID WOS:000313603200014
dc.type.publication Journal
dc.subject.otherAuthKeyword Caspase 3
dc.subject.otherAuthKeyword Caspase 9
dc.subject.otherAuthKeyword Hsp60
dc.subject.otherAuthKeyword PCNA
dc.subject.otherAuthKeyword TUNEL
dc.subject.otherChemCAS caspase 3, 169592-56-7
dc.subject.otherChemCAS caspase 9, 180189-96-2
dc.subject.otherChemCAS Caspase 3, 3.4.22.-
dc.subject.otherChemCAS Caspase 9, 3.4.22.-
dc.subject.otherChemCAS Chaperonin 60
dc.subject.otherChemCAS Proliferating Cell Nuclear Antigen
dc.subject.otherIndex caspase 3
dc.subject.otherIndex caspase 9
dc.subject.otherIndex chaperonin 60
dc.subject.otherIndex caspase 3
dc.subject.otherIndex caspase 9
dc.subject.otherIndex chaperonin 60
dc.subject.otherIndex cycline
dc.subject.otherIndex adolescent
dc.subject.otherIndex apoptosis
dc.subject.otherIndex article
dc.subject.otherIndex cell proliferation
dc.subject.otherIndex cell stress
dc.subject.otherIndex child
dc.subject.otherIndex clinical article
dc.subject.otherIndex female
dc.subject.otherIndex human
dc.subject.otherIndex human tissue
dc.subject.otherIndex immunohistochemistry
dc.subject.otherIndex immunoprecipitation
dc.subject.otherIndex male
dc.subject.otherIndex nick end labeling
dc.subject.otherIndex orthodontics
dc.subject.otherIndex school child
dc.subject.otherIndex tooth pulp
dc.subject.otherIndex traction therapy
dc.subject.otherIndex chemistry
dc.subject.otherIndex immunohistochemistry
dc.subject.otherIndex pathology
dc.subject.otherIndex Adolescent
dc.subject.otherIndex Apoptosis
dc.subject.otherIndex Caspase 3
dc.subject.otherIndex Caspase 9
dc.subject.otherIndex Chaperonin 60
dc.subject.otherIndex Child
dc.subject.otherIndex Dental Pulp
dc.subject.otherIndex Female
dc.subject.otherIndex Humans
dc.subject.otherIndex Immunohistochemistry
dc.subject.otherIndex Male
dc.subject.otherIndex Orthodontics, Corrective
dc.subject.otherIndex Proliferating Cell Nuclear Antigen
dc.subject.otherIndex Traction
dc.subject.otherKeywordPlus TOOTH MOVEMENT
dc.subject.otherKeywordPlus IMAGE-ANALYSIS
dc.subject.otherKeywordPlus BREAST-CANCER
dc.subject.otherKeywordPlus TREATED TEETH
dc.subject.otherKeywordPlus HSP60
dc.subject.otherKeywordPlus EXPRESSION
dc.subject.otherKeywordPlus QUANTIFICATION
dc.subject.otherKeywordPlus COMPLEX
dc.subject.otherKeywordPlus PRO-CASPASE-3
dc.subject.otherKeywordPlus INVOLVEMENT
dc.subject.otherWOS Endocrinology and Metabolism
dc.subject.otherWOS Immunology
dc.subject.otherWOS Medicine, Research and Experimental
dc.subject.otherWOS Physiology


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