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Orthodontic stress Bcl-2 modulation and human odontoblast survival

Show simple item record Leone A. Lipari L. Uzzo M.L. Spatola G.F. Provenzano S. Gerbino A. Jurjus A.R.
dc.contributor.editor Apr-2013 2017-09-07T07:00:01Z 2017-09-07T07:00:01Z 2013
dc.description.abstract This study assessed the effect of orthodontic traction on Bcl-2 expression and apoptosis in human dental pulp. It also explored, in absence of noxious stimuli the regeneration of odontoblasts during the entire life of the tooth. Twenty young patients, with Class II malocclusion and severe to moderate crowding, were referred for orthodontic assessment. Whole pulps were removed. Half the pulps were fixed, paraffin-embedded and processed for histology and immunohistochemistry using anti Bcl-2, Caspase 9 cleaved and Caspase 9 not cleaved antibodies. The rest of the samples, both orthodontically treated and not treated dental pulps, were immediately frozen at -80°C after the extraction and quantitative PCR was performed. Histology showed alterations in pulp microanatomy after 8 months of treatment. Immunohistochemistry depicted a decreasing expression of Bcl-2 in dental pulp over time in the non-treated while a very weak to absent Bcl-2 expression was detected in the orthodontically treated tissues. Active and non-active forms of Caspases, were expressed in both groups of dental pulp, however staining for the non active form was stronger than the corresponding cleaved form in all samples. The increased expression was detected mainly at nuclear level. Real time qPCR results correlated with those of immunohistochemistry and exhibited a decreasing expression of Bcl-2 in the treated samples. Orthodontic traction may inhibit the expression of Bcl-2, favoring the onset of apoptosis and leading us to conclude that the physical stress in the absence of noxious stimuli might make odontoblasts regeneration less likely. Copyright © by BIOLIFE, s.a.s.
dc.format.extent Pages: (417-425)
dc.language English
dc.publisher SILVA MARINA (TE)
dc.relation.ispartof Publication Name: Journal of Biological Regulators and Homeostatic Agents; Publication Year: 2013; Volume: 27; no. 2; Pages: (417-425);
dc.source Scopus
dc.title Orthodontic stress Bcl-2 modulation and human odontoblast survival
dc.type Article
dc.contributor.affiliation Leone, A., Section of Histology and Embryology, Palermo University, Medical and Dental School, Palermo, Italy
dc.contributor.affiliation Lipari, L., Section of Histology and Embryology, Palermo University, Medical and Dental School, Palermo, Italy
dc.contributor.affiliation Uzzo, M.L., Section of Histology and Embryology, Palermo University, Medical and Dental School, Palermo, Italy
dc.contributor.affiliation Spatola, G.F., Section of Histology and Embryology, Palermo University, Medical and Dental School, Palermo, Italy
dc.contributor.affiliation Provenzano, S., Departement of Surgery and Oncology, Palermo University, Medical and Dental School, Palermo, Italy
dc.contributor.affiliation Gerbino, A., Section of Histology and Embryology, Palermo University, Medical and Dental School, Palermo, Italy
dc.contributor.affiliation Jurjus, A.R., Department of Anatomy, Cell Biology and Physiology, American University of Beirut, Beirut, Lebanon
dc.contributor.authorAddress Lipari, L.; Section of Histology, Faculty of Medicine, University of Palermo, Palermo, Italy; email:
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Anatomy, Cell Biology and Physiological Sciences;
dc.contributor.authorDepartment Anatomy, Cell Biology and Physiological Sciences
dc.contributor.faculty Faculty of Medicine
dc.contributor.authorInitials Leone, A
dc.contributor.authorInitials Lipari, L
dc.contributor.authorInitials Uzzo, ML
dc.contributor.authorInitials Spatola, GF
dc.contributor.authorInitials Provenzano, S
dc.contributor.authorInitials Gerbino, A
dc.contributor.authorInitials Jurjus, AR
dc.contributor.authorReprintAddress Lipari, L (reprint author), Univ Palermo, Fac Med, Sect Histol BioNec, Palermo, Italy.
dc.contributor.authorUniversity American University of Beirut Medical Center
dc.description.cited Alhashimi N, 2001, AM J ORTHOD DENTOFAC, V119, P307, DOI 10.1067-mod.2001.110809; Bossy-Wetzel E, 1999, MUTAT RES-DNA REPAIR, V434, P243, DOI 10.1016-S0921-8777(99)00032-4; BRUDVIK P, 1994, EUR J ORTHODONT, V16, P249; Crawford ED, 2011, ANNU REV BIOCHEM, V80, P1055, DOI 10.1146-annurev-biochem-061809-121639; DAVIDOVITCH Z, 1988, DENT CLIN N AM, V32, P411; Garcia-Saez AJ, 2012, CELL DEATH DIFFER, V19, P1733, DOI 10.1038-cdd.2012.105; Green D, 1998, TRENDS CELL BIOL, V8, P267, DOI 10.1016-S0962-8924(98)01273-2; Jacinto-Aleman LF, 2010, J ORAL PATHOL MED, V39, P709, DOI 10.1111-j.1600-0714.2010.00918.x; Jager A, 2005, EUR J ORTHODONT, V27, P1, DOI 10.1093-ejo-cjh089; Jeong SY, 2008, BMB REP, V41, P11; Kalia S, 2009, MOL CELL BIOCHEM, V322, P43, DOI 10.1007-s11010-008-9938-7; Karube H, 2009, J DENT RES, V88, P461, DOI 10.1177-0022034509334771; KERR JFR, 1972, BRIT J CANCER, V26, P239, DOI 10.1038-bjc.1972.33; Kirkeby S, 2005, J IMMUNOL METHODS, V301, P102, DOI 10.1016-j.jim.2005.04.006; Krishnan V., 2006, AM J ORTHOD DENTOFAC, V129; Kuida K, 1998, CELL, V94, P325, DOI 10.1016-S0092-8674(00)81476-2; Lamkanfi M, 2007, CELL DEATH DIFFER, V14, P44, DOI 10.1038-sj.cdd.4402047; Lehr HA, 1997, J HISTOCHEM CYTOCHEM, V45, P1559; Leone A, 2002, ANGLE ORTHOD, V79, P1119; Leone A, 2002, Bull Group Int Rech Sci Stomatol Odontol, V44, P57; Liu JR, 2002, CANCER RES, V62, P924; Matalova E, 2012, OMICS, V16, P60, DOI 10.1089-omi.2011.0039; Mesgouez C, 2006, INT ENDOD J, V39, P453, DOI 10.1111-j.1365-2591.2006.01089.x; Mitsiadis TA, 2008, EXP CELL RES, V314, P869, DOI 10.1016-j.yexcr.2007.11.001; RYGH P, 1986, AM J ORTHOD DENTOFAC, V89, P453, DOI 10.1016-0002-9416(86)90001-1; Setkova J, 2007, ARCH ORAL BIOL, V52, P15; Smith A J, 2001, Adv Dent Res, V15, P51; Soengas MS, 2001, NATURE, V409, P207, DOI 10.1038-35051606; Stadelmann C, 2000, CELL TISSUE RES, V301, P19, DOI 10.1007-s004410000203; Theoleyre S, 2004, CYTOKINE GROWTH F R, V15, P457, DOI 10.1016-j.cytogfr.2004.06.004; Tzannetou S, 2008, AM J ORTHOD DENTOFAC, V133, P699, DOI 10.1016-j.ajodo.2006.03.044; Wylie A., 1980, INT REV CYTOL, V68, P251; Yamaguchi M, 2009, ORTHOD CRANIOFAC RES, V12, P113, DOI 10.1111-j.1601-6343.2009.01444.x; Yan XL, 2009, ANGLE ORTHOD, V79, P1126, DOI [10.2319-100508-519R.1, 10.2319-100508-519.1]; Zhang WJ, 2012, ARCH ORAL BIOL, V57, P285, DOI 10.1016-j.archoralbio.2011.08.022
dc.description.citedTotWOSCount 0
dc.description.citedWOSCount 0
dc.format.extentCount 9
dc.identifier.coden JBRAE
dc.identifier.pubmedID 23830392
dc.identifier.scopusID 84883481801
dc.publisher.address VIA S STEFANO 39 BIS, 64029 SILVA MARINA (TE), ITALY
dc.relation.ispartOfISOAbbr J. Biol. Regul. Homeost. Agents
dc.relation.ispartOfIssue 2
dc.relation.ispartofPubTitle Journal of Biological Regulators and Homeostatic Agents
dc.relation.ispartofPubTitleAbbr J. Biol. Regul. Homeostatic Agents
dc.relation.ispartOfVolume 27
dc.source.ID WOS:000322290700014
dc.type.publication Journal
dc.subject.otherAuthKeyword Bcl-2
dc.subject.otherAuthKeyword Caspase 9
dc.subject.otherAuthKeyword Odontoblasts
dc.subject.otherChemCAS caspase 9, 180189-96-2
dc.subject.otherChemCAS protein bcl 2, 219306-68-0
dc.subject.otherChemCAS Caspase 9, 3.4.22.-
dc.subject.otherChemCAS Proto-Oncogene Proteins c-bcl-2
dc.subject.otherIndex caspase 9
dc.subject.otherIndex paraffin
dc.subject.otherIndex protein bcl 2
dc.subject.otherIndex caspase 9
dc.subject.otherIndex protein bcl 2
dc.subject.otherIndex adolescent
dc.subject.otherIndex apoptosis
dc.subject.otherIndex article
dc.subject.otherIndex child
dc.subject.otherIndex clinical article
dc.subject.otherIndex controlled study
dc.subject.otherIndex crowding (tooth)
dc.subject.otherIndex female
dc.subject.otherIndex histology
dc.subject.otherIndex human
dc.subject.otherIndex human tissue
dc.subject.otherIndex immunohistochemistry
dc.subject.otherIndex male
dc.subject.otherIndex malocclusion
dc.subject.otherIndex modulation
dc.subject.otherIndex odontoblast
dc.subject.otherIndex orthodontics
dc.subject.otherIndex physical stress
dc.subject.otherIndex protein expression
dc.subject.otherIndex real time polymerase chain reaction
dc.subject.otherIndex regeneration
dc.subject.otherIndex school child
dc.subject.otherIndex tooth pulp
dc.subject.otherIndex traction therapy
dc.subject.otherIndex cell survival
dc.subject.otherIndex chemistry
dc.subject.otherIndex cytology
dc.subject.otherIndex down regulation
dc.subject.otherIndex mechanical stress
dc.subject.otherIndex metabolism
dc.subject.otherIndex physiology
dc.subject.otherIndex tooth pulp
dc.subject.otherIndex Adolescent
dc.subject.otherIndex Caspase 9
dc.subject.otherIndex Cell Survival
dc.subject.otherIndex Child
dc.subject.otherIndex Dental Pulp
dc.subject.otherIndex Down-Regulation
dc.subject.otherIndex Female
dc.subject.otherIndex Humans
dc.subject.otherIndex Male
dc.subject.otherIndex Odontoblasts
dc.subject.otherIndex Orthodontics
dc.subject.otherIndex Proto-Oncogene Proteins c-bcl-2
dc.subject.otherIndex Regeneration
dc.subject.otherIndex Stress, Mechanical
dc.subject.otherKeywordPlus TOOTH MOVEMENT
dc.subject.otherKeywordPlus APOPTOSIS
dc.subject.otherKeywordPlus ACTIVATION
dc.subject.otherKeywordPlus CASPASES
dc.subject.otherKeywordPlus ODONTOGENESIS
dc.subject.otherKeywordPlus MITOCHONDRIA
dc.subject.otherKeywordPlus CYTOKINES
dc.subject.otherKeywordPlus CANCER
dc.subject.otherKeywordPlus REPAIR
dc.subject.otherKeywordPlus CELLS
dc.subject.otherWOS Endocrinology and Metabolism
dc.subject.otherWOS Immunology
dc.subject.otherWOS Medicine, Research and Experimental
dc.subject.otherWOS Physiology

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