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Kefir exhibits anti-proliferative and pro-apoptotic effects on colon adenocarcinoma cells with no significant effects on cell migration and invasion

Show simple item record Khoury N. El-Hayek S. Tarras O. El-Sabban M. El-Sibai M. Rizk S.
dc.contributor.editor 2014 2017-09-07T07:00:03Z 2017-09-07T07:00:03Z 2014
dc.identifier 10.3892/ijo.2014.2635
dc.identifier.issn 10196439
dc.description.abstract Kefir, a fermented milk product, exhibits anti-tumoral activity in vivo; yet its mechanism of action remains elusive. Recent studies have focused on the mechanism of action of kefir on cancer cells in vitro. The current study aims at examining the effect of kefir on cell survival, proliferation, and motility of colorectal cancer (CRC ) cells. Kefir's anti-cancer potential was tested on CRC cell lines, Caco-2 and HT- 29, through cytotoxicity, proliferation, and apoptotic assays. The expression of certain genes involved in proliferation and apoptosis was measured using reverse transcriptase-polymerase chain reaction (RT- PCR ) and western blotting. To assess the effect of kefir on cancer metastasis, wound-healing and time-lapse movies, in addition to collagen-based invasion assay, were used. The results show that cell-free fractions of kefir exhibit an anti-proliferative effect on Caco-2 and HT- 29 cells. Analysis of DNA content by flow cytometry revealed the ability of kefir to induce cell cycle arrest at the G1 phase. Kefir was also found to induce apoptosis, as seen by cell death ELI SA. Results from RT- PCR showed that kefir decreases the expression of transforming growth factor a (TGF-a); a nd t ransforming g rowth factor-β1 (TGF-β1) in HT- 29 cells. Western blotting results revealed an upregulation in Bax:Bcl-2 ratio, confirming the pro-apoptotic effect of kefir, and an increase in p53 independent-p21 expression upon kefir treatment. MMP expression was not altered by kefir treatment. Furthermore, results from time-lapse motility movies, wound-healing, and invasion assays showed no effect on the motility of colorectal as well as breast (MCF-7 and MB-MDA-231) cancer cells upon kefir treatment. Our data suggest that kefir is able to inhibit the proliferation and induce apoptosis in HT- 29 and Caco-2 CRC cells, yet it does not exhibit a significant effect on the motility and invasion of these cells in vitro.
dc.format.extent Pages: (2117-2127)
dc.language English
dc.publisher Spandidos Publications; ATHENS
dc.relation.ispartof Publication Name: International Journal of Oncology; Publication Year: 2014; Volume: 45; no. 5; Pages: (2117-2127);
dc.source Scopus
dc.title Kefir exhibits anti-proliferative and pro-apoptotic effects on colon adenocarcinoma cells with no significant effects on cell migration and invasion
dc.type Article
dc.contributor.affiliation Khoury, N., Department of Natural Sciences, Lebanese American University, P.O. Box 13-5053Chouran, Beirut, Lebanon
dc.contributor.affiliation El-Hayek, S., Department of Natural Sciences, Lebanese American University, P.O. Box 13-5053Chouran, Beirut, Lebanon
dc.contributor.affiliation Tarras, O., Department of Natural Sciences, Lebanese American University, P.O. Box 13-5053Chouran, Beirut, Lebanon
dc.contributor.affiliation El-Sabban, M., Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of BeirutBeirut, Lebanon
dc.contributor.affiliation El-Sibai, M., Department of Natural Sciences, Lebanese American University, P.O. Box 13-5053Chouran, Beirut, Lebanon
dc.contributor.affiliation Rizk, S., Department of Natural Sciences, Lebanese American University, P.O. Box 13-5053Chouran, Beirut, Lebanon
dc.contributor.authorAddress Rizk, S.; Department of Natural Sciences, Lebanese American University, P.O. Box 13-5053, Lebanon
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Anatomy, Cell Biology and Physiological Sciences;
dc.contributor.authorDepartment Anatomy, Cell Biology and Physiological Sciences
dc.contributor.faculty Faculty of Medicine
dc.contributor.authorInitials Khoury, N
dc.contributor.authorInitials El-Hayek, S
dc.contributor.authorInitials Tarras, O
dc.contributor.authorInitials El-Sabban, M
dc.contributor.authorInitials El-Sibai, M
dc.contributor.authorInitials Rizk, S
dc.contributor.authorReprintAddress Rizk, S (reprint author), Lebanese Amer Univ, Dept Nat Sci, POB 13-5053, Beirut 11022801, Lebanon.
dc.contributor.authorUniversity American University of Beirut Medical Center
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dc.description.citedTotWOSCount 0
dc.description.citedWOSCount 0
dc.format.extentCount 11
dc.identifier.coden IJONE
dc.identifier.scopusID 84907212867
dc.publisher.address POB 18179, ATHENS, 116 10, GREECE
dc.relation.ispartOfISOAbbr Int. J. Oncol.
dc.relation.ispartOfIssue 5
dc.relation.ispartofPubTitle International Journal of Oncology
dc.relation.ispartofPubTitleAbbr Int. J. Oncol.
dc.relation.ispartOfVolume 45
dc.source.ID WOS:000342713900038
dc.type.publication Journal
dc.subject.otherAuthKeyword Apoptosis
dc.subject.otherAuthKeyword Cell invasion
dc.subject.otherAuthKeyword Cell motility
dc.subject.otherAuthKeyword Colorectal cancer
dc.subject.otherAuthKeyword Kefir
dc.subject.otherKeywordPlus GROWTH-FACTOR-ALPHA
dc.subject.otherKeywordPlus COLORECTAL-CANCER
dc.subject.otherKeywordPlus ANTITUMOR-ACTIVITY
dc.subject.otherKeywordPlus MILK-KEFIR
dc.subject.otherKeywordPlus IN-VITRO
dc.subject.otherKeywordPlus RECEPTOR
dc.subject.otherKeywordPlus METASTASIS
dc.subject.otherKeywordPlus MICE
dc.subject.otherKeywordPlus KINASE
dc.subject.otherWOS Oncology

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