AUB ScholarWorks

The effect of endotoxin on functional parameters of mammary CID-9 cells

Show simple item record Safieh-Garabedian B. Mouneimne G.M. El-Jouni W. Khattar M. Talhouk R.
dc.contributor.editor 2004 2017-10-03T15:43:24Z 2017-10-03T15:43:24Z 2004
dc.identifier.issn 14701626
dc.description.abstract The effect of endotoxin on mammary CID-9 cells, which differentiate in culture and express β-casein, was investigated. Cells in culture supplemented with lactogenic hormones and dripped with EMS-Matrix (EMS-drip), were treated daily with endotoxin (0.5-500 μg-ml). Endotoxin at concentrations of less or equal to 10 μg-ml did not affect cell growth and viability up to 5 days post endotoxin treatment. Endotoxin (0.01-10 μg-ml) was added to the culture medium, upon confluence, and functional parameters were examined within 48 h post endotoxin treatment. Nuclear factor-κB (NF-κB) (p52) increased in nuclear extracts from endotoxin-stimulated cells within 1 h of treatment, while β-casein mRNA and protein expression decreased in a concentration-dependent manner at 24 and 48 h post treatment. Zymography showed that the 72 and 92 kDa gelatinase activity increased in cells at 24 and 48 h post endotoxin treatment at 10 and 50 μg-ml. At the latter concentration, the active form of 72 kDa gelatinase was induced at 48 h. Interleukin-6 and tumor necrosis factor-α levels increased at 1-3 h post endotoxin treatment and peaked at 6 h in cells on plastic and EHS-drip. Nerve growth factor (NGF) levels increased in control and endotoxin-treated cells in a time-dependent manner, and endotoxin increased NGF levels in culture at 6 and 9 h post endotoxin treatment. This study shows that endotoxin activated NF-κB, suppressed β-casein expression and upregulated gelatinases, cytokines and NGF. This model could be used to investigate the role of mammary cells in initiating and propagating inflammation and to test candidate molecules for potential anti-inflammatory properties. © 2004 Society for Reproduction and Fertility.
dc.format.extent Pages: (397-406)
dc.language English
dc.publisher BRISTOL
dc.relation.ispartof Publication Name: Reproduction; Publication Year: 2004; Volume: 127; no. 3; Pages: (397-406);
dc.source Scopus
dc.title The effect of endotoxin on functional parameters of mammary CID-9 cells
dc.type Review
dc.contributor.affiliation Safieh-Garabedian, B., Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon
dc.contributor.affiliation Mouneimne, G.M., Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon, Dept. of Anatomy-Structural Biology, Albert Einstein College of Medicine, New York, NY 10461, United States
dc.contributor.affiliation El-Jouni, W., Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon
dc.contributor.affiliation Khattar, M., Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon
dc.contributor.affiliation Talhouk, R., Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon
dc.contributor.authorAddress Talhouk, R.; Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon; email:
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Arts and Sciences; Department: Biology;
dc.contributor.authorDepartment Biology
dc.contributor.authorFaculty Faculty of Arts and Sciences
dc.contributor.authorInitials Safieh-Garabedian, B
dc.contributor.authorInitials Mouneimne, GM
dc.contributor.authorInitials El-Jouni, W
dc.contributor.authorInitials Khattar, M
dc.contributor.authorInitials Talhouk, R
dc.contributor.authorReprintAddress Talhouk, R (reprint author), Amer Univ Beirut, Fac Arts and Sci, Dept Biol, POB 11-0236, Beirut, Lebanon.
dc.contributor.authorUniversity American University of Beirut
dc.description.cited [Anonymous], 2002, SPSS WIND REL 11 5; Baldwin AS, 1996, ANNU REV IMMUNOL, V14, P649, DOI 10.1146-annurev.immunol.14.1.649; Baldwin AS, 2001, J CLIN INVEST, V107, P3, DOI 10.1172-JCI11891; Bondeson J, 1999, P NATL ACAD SCI USA, V96, P5668, DOI 10.1073-pnas.96.10.5668; Boudjellab N, 1998, AM J VET RES, V59, P1563; BOUDREAU N, 1995, SCIENCE, V267, P891, DOI 10.1126-science.7531366; CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1006-abio.1987.9999; COLDITZ IG, 1990, J LEUKOCYTE BIOL, V48, P129; COLDITZ IG, 1987, IMMUNOL CELL BIOL, V65, P437, DOI 10.1038-icb.1987.52; Desprez Pierre-Yves, 1993, Molecular and Cellular Differentiation, V1, P99; DHONDT G, 1977, J DAIRY RES, V44, P433; Doppler W, 2000, ADV EXP MED BIOL, V480, P139; El-Sabban ME, 2003, J CELL SCI, V116, P3531, DOI 10.1242-jcs.00656; EPSTEIN J, 1990, IN VITRO CELL DEV, V26, P112; GEARING AJH, 1994, NATURE, V370, P555, DOI 10.1038-370555a0; Geymayer S, 2000, FASEB J, V14, P1159; Haddad JJE, 2001, BIOCHEM J, V355, P29; Han ZN, 1998, AUTOIMMUNITY, V28, P197, DOI 10.3109-08916939808995367; Homaidan FR, 1999, MEDIAT INFLAMM, V8, P189; HOMAIDAN FR, 1995, MEDIAT INFLAMM, V4, P61, DOI 10.1155-S0962935195000111; INCH S, 1995, PRACTITIONER, V239, P472; JACKSON JA, 1990, J DAIRY SCI, V73, P627; Kim HG, 2000, BIOCHEM BIOPH RES CO, V269, P401; LEE JD, 1993, P NATL ACAD SCI USA, V90, P9930, DOI 10.1073-pnas.90.21.9930; LENGEMANN FW, 1987, J DAIRY SCI, V70, P201; Martin CA, 1998, CELL ADHES COMMUN, V5, P437, DOI 10.3109-15419069809005602; Martin CA, 1998, CELL ADHES COMMUN, V5, P83, DOI 10.3109-15419069809040283; MATTERN T, 1994, J IMMUNOL, V153, P2996; Mengshol JA, 2000, ARTHRITIS RHEUM, V43, P801, DOI 10.1002-1529-0131(200004)43:4801::AID-ANR103.0.CO;2-4; MORRIS DD, 1992, AM J VET RES, V53, P1298; MORRISON DC, 1994, ASM NEWS, V60, P479; Mullarkey M, 2003, J PHARMACOL EXP THER, V304, P1093, DOI 10.1124-jpet.102.044487; Okada H, 1997, J VET MED SCI, V59, P503, DOI 10.1292-jvms.59.503; Okada H, 1999, J VET MED SCI, V61, P33, DOI 10.1292-jvms.61.33; OLIVER SP, 1982, J DAIRY SCI, V65, P801; Outteridge P M, 1981, Adv Exp Med Biol, V137, P513; PERSSON K, 1992, J VET MED B, V39, P165; PUGIN J, 1993, P NATL ACAD SCI USA, V90, P2744, DOI 10.1073-pnas.90.7.2744; Rahman I, 2000, BIOCHEM PHARMACOL, V60, P1041, DOI 10.1016-S0006-2952(00)00392-0; READ MA, 1993, P NATL ACAD SCI USA, V90, P9887, DOI 10.1073-pnas.90.21.9887; Riollet C, 2000, ADV EXP MED BIOL, V480, P247; SafiehGarabedian B, 1997, BRIT J PHARMACOL, V121, P1619, DOI 10.1038-sj.bjp.0701313; SAFIEHGARABEDIAN B, 1995, BRIT J PHARMACOL, V115, P1265; SafiehGarabedian B, 1996, NEUROPHARMACOLOGY, V35, P599, DOI 10.1016-0028-3908(96)84630-2; SCHANBACHER FL, 1993, J DAIRY SCI, V76, P3812, DOI 10.3168-jds.S0022-0302(93)77725-5; Schanbacher FL, 1997, LIVEST PROD SCI, V50, P105, DOI 10.1016-S0301-6226(97)00082-1; SCHMIDHAUSER C, 1990, P NATL ACAD SCI USA, V87, P9118, DOI 10.1073-pnas.87.23.9118; Semba RD, 1999, NUTR REV, V57, P146; SHUSTER DE, 1993, AM J VET RES, V54, P80; Shuster DE, 1997, INFECT IMMUN, V65, P3286; SIBLEY CH, 1988, J BIOL CHEM, V263, P5098; STRANGE R, 1992, DEVELOPMENT, V115, P49; SYMPSON CJ, 1994, J CELL BIOL, V125, P681, DOI 10.1083-jcb.125.3.681; Tak PP, 2001, J CLIN INVEST, V107, P7, DOI 10.1172-JCI11830; TALHOUK R, 2000, J ENDOTOXIN RES, V6, pA124; TALHOUK R, 2001, DOMEST ANIM ENDOCRIN, V3, P143; TALHOUK RS, 1991, DEVELOPMENT, V112, P439; TALHOUK RS, 1992, J CELL BIOL, V118, P1271, DOI 10.1083-jcb.118.5.1271; Talhouk RS, 2000, PAIN, V84, P397, DOI 10.1016-S0304-3959(99)00238-9; Tarraf C, 2003, FOOD ADDIT CONTAM, V20, P819, DOI 10.1080-0265203031000138231; Tomita T, 2000, RHEUMATOLOGY, V39, P749, DOI 10.1093-rheumatology-39.7.749; VONANDRIAN UH, 1991, P NATL ACAD SCI USA, V88, P7538, DOI 10.1073-pnas.88.17.7538; Waller KP, 2000, ADV EXP MED BIOL, V480, P231; WILLE JJ, 1992, J CELL PHYSIOL, V150, P52, DOI 10.1002-jcp.1041500108; Yang J, 2000, J ANIM SCI, V78, P3114; Yao PM, 1997, AM J PHYSIOL-LUNG C, V273, pL866; Yokooo T, 1996, AM J PHYSIOL, V270, P123; Zhang YH, 1998, J IMMUNOL, V161, P3071
dc.description.citedCount 10
dc.description.citedTotWOSCount 12
dc.description.citedWOSCount 12
dc.format.extentCount 10
dc.identifier.coden RCUKB
dc.identifier.pubmedID 15016959
dc.identifier.scopusID 1842538841
dc.relation.ispartOfISOAbbr Reproduction
dc.relation.ispartOfIssue 3
dc.relation.ispartofPubTitle Reproduction
dc.relation.ispartofPubTitleAbbr Reproduction
dc.relation.ispartOfVolume 127
dc.source.ID WOS:000220568300011
dc.type.publication Journal
dc.subject.otherChemCAS gelatinase, 9040-48-6
dc.subject.otherChemCAS nerve growth factor, 9061-61-4
dc.subject.otherChemCAS prolactin, 12585-34-1, 50647-00-2, 9002-62-4
dc.subject.otherChemCAS Caseins
dc.subject.otherChemCAS Cytokines
dc.subject.otherChemCAS Endotoxins
dc.subject.otherChemCAS Gelatinases, EC 3.4.24.-
dc.subject.otherChemCAS Nerve Growth Factor, 9061-61-4
dc.subject.otherChemCAS NF-kappa B
dc.subject.otherIndex beta casein
dc.subject.otherIndex Escherichia coli endotoxin
dc.subject.otherIndex gelatinase
dc.subject.otherIndex immunoglobulin enhancer binding protein
dc.subject.otherIndex interleukin 6
dc.subject.otherIndex nerve growth factor
dc.subject.otherIndex prolactin
dc.subject.otherIndex tumor necrosis factor alpha
dc.subject.otherIndex animal cell
dc.subject.otherIndex breast cell
dc.subject.otherIndex cell culture
dc.subject.otherIndex cell differentiation
dc.subject.otherIndex cell growth
dc.subject.otherIndex cell viability
dc.subject.otherIndex cellular parameters
dc.subject.otherIndex concentration (parameters)
dc.subject.otherIndex controlled study
dc.subject.otherIndex culture medium
dc.subject.otherIndex enzyme activity
dc.subject.otherIndex gel mobility shift assay
dc.subject.otherIndex inflammation
dc.subject.otherIndex mouse
dc.subject.otherIndex nonhuman
dc.subject.otherIndex priority journal
dc.subject.otherIndex protein expression
dc.subject.otherIndex review
dc.subject.otherIndex upregulation
dc.subject.otherIndex zymography
dc.subject.otherIndex Animals
dc.subject.otherIndex Blotting, Western
dc.subject.otherIndex Caseins
dc.subject.otherIndex Cell Line
dc.subject.otherIndex Cell Survival
dc.subject.otherIndex Cytokines
dc.subject.otherIndex Electrophoretic Mobility Shift Assay
dc.subject.otherIndex Endotoxins
dc.subject.otherIndex Enzyme-Linked Immunosorbent Assay
dc.subject.otherIndex Female
dc.subject.otherIndex Gelatinases
dc.subject.otherIndex Mammary Glands, Animal
dc.subject.otherIndex Mastitis
dc.subject.otherIndex Mice
dc.subject.otherIndex Nerve Growth Factor
dc.subject.otherIndex NF-kappa B
dc.subject.otherKeywordPlus NF-KAPPA-B
dc.subject.otherKeywordPlus GAP JUNCTIONAL COMMUNICATION
dc.subject.otherKeywordPlus INTESTINAL EPITHELIAL-CELLS
dc.subject.otherKeywordPlus NECROSIS-FACTOR-ALPHA
dc.subject.otherKeywordPlus LACTATING DAIRY-COWS
dc.subject.otherKeywordPlus NERVE GROWTH-FACTOR
dc.subject.otherKeywordPlus GENE-EXPRESSION
dc.subject.otherKeywordPlus EXTRACELLULAR-MATRIX
dc.subject.otherKeywordPlus ESCHERICHIA-COLI
dc.subject.otherKeywordPlus INFLAMMATORY HYPERALGESIA
dc.subject.otherWOS Developmental Biology
dc.subject.otherWOS Reproductive Biology

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search AUB ScholarWorks


My Account