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The effect of endotoxin on functional parameters of mammary CID-9 cells

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dc.contributor.author Safieh-Garabedian B.
dc.contributor.author Mouneimne G.M.
dc.contributor.author El-Jouni W.
dc.contributor.author Khattar M.
dc.contributor.author Talhouk R.
dc.contributor.editor
dc.date 2004
dc.date.accessioned 2017-10-03T15:43:24Z
dc.date.available 2017-10-03T15:43:24Z
dc.date.issued 2004
dc.identifier
dc.identifier.isbn
dc.identifier.issn 14701626
dc.identifier.uri http://hdl.handle.net/10938/12416
dc.description.abstract The effect of endotoxin on mammary CID-9 cells, which differentiate in culture and express β-casein, was investigated. Cells in culture supplemented with lactogenic hormones and dripped with EMS-Matrix (EMS-drip), were treated daily with endotoxin (0.5-500 μg-ml). Endotoxin at concentrations of less or equal to 10 μg-ml did not affect cell growth and viability up to 5 days post endotoxin treatment. Endotoxin (0.01-10 μg-ml) was added to the culture medium, upon confluence, and functional parameters were examined within 48 h post endotoxin treatment. Nuclear factor-κB (NF-κB) (p52) increased in nuclear extracts from endotoxin-stimulated cells within 1 h of treatment, while β-casein mRNA and protein expression decreased in a concentration-dependent manner at 24 and 48 h post treatment. Zymography showed that the 72 and 92 kDa gelatinase activity increased in cells at 24 and 48 h post endotoxin treatment at 10 and 50 μg-ml. At the latter concentration, the active form of 72 kDa gelatinase was induced at 48 h. Interleukin-6 and tumor necrosis factor-α levels increased at 1-3 h post endotoxin treatment and peaked at 6 h in cells on plastic and EHS-drip. Nerve growth factor (NGF) levels increased in control and endotoxin-treated cells in a time-dependent manner, and endotoxin increased NGF levels in culture at 6 and 9 h post endotoxin treatment. This study shows that endotoxin activated NF-κB, suppressed β-casein expression and upregulated gelatinases, cytokines and NGF. This model could be used to investigate the role of mammary cells in initiating and propagating inflammation and to test candidate molecules for potential anti-inflammatory properties. © 2004 Society for Reproduction and Fertility.
dc.format.extent
dc.format.extent Pages: (397-406)
dc.language English
dc.publisher BRISTOL
dc.relation.ispartof Publication Name: Reproduction; Publication Year: 2004; Volume: 127; no. 3; Pages: (397-406);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other
dc.title The effect of endotoxin on functional parameters of mammary CID-9 cells
dc.type Review
dc.contributor.affiliation Safieh-Garabedian, B., Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon
dc.contributor.affiliation Mouneimne, G.M., Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon, Dept. of Anatomy-Structural Biology, Albert Einstein College of Medicine, New York, NY 10461, United States
dc.contributor.affiliation El-Jouni, W., Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon
dc.contributor.affiliation Khattar, M., Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon
dc.contributor.affiliation Talhouk, R., Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon
dc.contributor.authorAddress Talhouk, R.; Department of Biology, Faculty of Arts and Science, American University of Beirut, PO Box 11-0236, Beirut, Lebanon; email: rtalhouk@aub.edu.lb
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Arts and Sciences; Department: Biology;
dc.contributor.authorDepartment Biology
dc.contributor.authorDivision
dc.contributor.authorEmail rtalhouk@aub.edu.lb
dc.contributor.authorFaculty Faculty of Arts and Sciences
dc.contributor.authorInitials Safieh-Garabedian, B
dc.contributor.authorInitials Mouneimne, GM
dc.contributor.authorInitials El-Jouni, W
dc.contributor.authorInitials Khattar, M
dc.contributor.authorInitials Talhouk, R
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Talhouk, R (reprint author), Amer Univ Beirut, Fac Arts and Sci, Dept Biol, POB 11-0236, Beirut, Lebanon.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut
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dc.description.citedCount 10
dc.description.citedTotWOSCount 12
dc.description.citedWOSCount 12
dc.format.extentCount 10
dc.identifier.articleNo
dc.identifier.coden RCUKB
dc.identifier.pubmedID 15016959
dc.identifier.scopusID 1842538841
dc.identifier.url
dc.publisher.address EURO HOUSE, 22 APEX COURT WOODLANDS, BRADLEY STOKE, BRISTOL BS32 4JT, ENGLAND
dc.relation.ispartofConference
dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr Reproduction
dc.relation.ispartOfIssue 3
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Reproduction
dc.relation.ispartofPubTitleAbbr Reproduction
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 127
dc.source.ID WOS:000220568300011
dc.type.publication Journal
dc.subject.otherAuthKeyword
dc.subject.otherChemCAS gelatinase, 9040-48-6
dc.subject.otherChemCAS nerve growth factor, 9061-61-4
dc.subject.otherChemCAS prolactin, 12585-34-1, 50647-00-2, 9002-62-4
dc.subject.otherChemCAS Caseins
dc.subject.otherChemCAS Cytokines
dc.subject.otherChemCAS Endotoxins
dc.subject.otherChemCAS Gelatinases, EC 3.4.24.-
dc.subject.otherChemCAS Nerve Growth Factor, 9061-61-4
dc.subject.otherChemCAS NF-kappa B
dc.subject.otherIndex beta casein
dc.subject.otherIndex Escherichia coli endotoxin
dc.subject.otherIndex gelatinase
dc.subject.otherIndex immunoglobulin enhancer binding protein
dc.subject.otherIndex interleukin 6
dc.subject.otherIndex nerve growth factor
dc.subject.otherIndex prolactin
dc.subject.otherIndex tumor necrosis factor alpha
dc.subject.otherIndex animal cell
dc.subject.otherIndex breast cell
dc.subject.otherIndex cell culture
dc.subject.otherIndex cell differentiation
dc.subject.otherIndex cell growth
dc.subject.otherIndex cell viability
dc.subject.otherIndex cellular parameters
dc.subject.otherIndex concentration (parameters)
dc.subject.otherIndex controlled study
dc.subject.otherIndex culture medium
dc.subject.otherIndex enzyme activity
dc.subject.otherIndex gel mobility shift assay
dc.subject.otherIndex inflammation
dc.subject.otherIndex mouse
dc.subject.otherIndex nonhuman
dc.subject.otherIndex priority journal
dc.subject.otherIndex protein expression
dc.subject.otherIndex review
dc.subject.otherIndex upregulation
dc.subject.otherIndex zymography
dc.subject.otherIndex Animals
dc.subject.otherIndex Blotting, Western
dc.subject.otherIndex Caseins
dc.subject.otherIndex Cell Line
dc.subject.otherIndex Cell Survival
dc.subject.otherIndex Cytokines
dc.subject.otherIndex Electrophoretic Mobility Shift Assay
dc.subject.otherIndex Endotoxins
dc.subject.otherIndex Enzyme-Linked Immunosorbent Assay
dc.subject.otherIndex Female
dc.subject.otherIndex Gelatinases
dc.subject.otherIndex Mammary Glands, Animal
dc.subject.otherIndex Mastitis
dc.subject.otherIndex Mice
dc.subject.otherIndex Nerve Growth Factor
dc.subject.otherIndex NF-kappa B
dc.subject.otherKeywordPlus NF-KAPPA-B
dc.subject.otherKeywordPlus GAP JUNCTIONAL COMMUNICATION
dc.subject.otherKeywordPlus INTESTINAL EPITHELIAL-CELLS
dc.subject.otherKeywordPlus NECROSIS-FACTOR-ALPHA
dc.subject.otherKeywordPlus LACTATING DAIRY-COWS
dc.subject.otherKeywordPlus NERVE GROWTH-FACTOR
dc.subject.otherKeywordPlus GENE-EXPRESSION
dc.subject.otherKeywordPlus EXTRACELLULAR-MATRIX
dc.subject.otherKeywordPlus ESCHERICHIA-COLI
dc.subject.otherKeywordPlus INFLAMMATORY HYPERALGESIA
dc.subject.otherWOS Developmental Biology
dc.subject.otherWOS Reproductive Biology


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