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Gap junctions mediate STAT5-independent β-casein expression in CID-9 mammary epithelial cells

Show simple item record Talhouk R.S. Khalil A.A. Bajjani R. Rahme G.J. El-Sabban M.E.
dc.contributor.editor Oct-2011 2017-10-03T15:43:25Z 2017-10-03T15:43:25Z 2011
dc.identifier 10.3109/15419061.2011.639468
dc.identifier.issn 15419061
dc.description.abstract Crosstalk between gap junction intracellular communication (GJIC), STAT5 and OCT-1 in gap junction (GJ)-dependent β-casein expression was investigated. CID-9 mammary cells plated with prolactin on non-adherent substratum (poly-HEMA) expressed β-casein independent of STAT5 only in the presence of the GJIC inducer, cAMP. Nuclear STAT5 levels were not detectable. By contrast, cells on EHS-drip expressed β-casein in a STAT5-dependent manner and nuclear STAT5 levels were up-regulated. A 75 kDa OCT-1 isoform was detected in conditions that induced β-casein expression regardless of substratum. Interestingly, 40 and 28 kDa OCT-1 isoforms were induced in cells on polyHEMA with cAMP. Electrophoretic mobility shift assays (EMSA) for OCT-1 revealed two band shifts in cells on polyHEMA with cAMP and on EHS-drip, which were repressed by the GJIC inhibitor, 18α-GA. These studies demonstrated that mammary cells on polyHEMA expressed β-casein in response to prolactin in a pathway that involves GJIC and OCT-1 and is independent of STAT5 nuclear translocation. © 2011 Informa Healthcare USA, Inc.
dc.format.extent Pages: (104-116)
dc.language English
dc.publisher LONDON
dc.relation.ispartof Publication Name: Cell Communication and Adhesion; Publication Year: 2011; Volume: 18; no. 5; Pages: (104-116);
dc.source Scopus
dc.title Gap junctions mediate STAT5-independent β-casein expression in CID-9 mammary epithelial cells
dc.type Article
dc.contributor.affiliation Talhouk, R.S., Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Beirut P.O. Box 11-0236, Lebanon
dc.contributor.affiliation Khalil, A.A., Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Beirut P.O. Box 11-0236, Lebanon
dc.contributor.affiliation Bajjani, R., Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Beirut P.O. Box 11-0236, Lebanon
dc.contributor.affiliation Rahme, G.J., Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Beirut P.O. Box 11-0236, Lebanon
dc.contributor.affiliation El-Sabban, M.E., Department of Human Morphology, American University of Beirut, Beirut, Lebanon
dc.contributor.authorAddress Talhouk, R.S.; Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Beirut P.O. Box 11-0236, Lebanon; email:
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Arts and Sciences; Department: Biology;
dc.contributor.authorDepartment Biology
dc.contributor.faculty Faculty of Arts and Sciences
dc.contributor.authorInitials Talhouk, RS
dc.contributor.authorInitials Khalil, AA
dc.contributor.authorInitials Bajjani, R
dc.contributor.authorInitials Rahme, GJ
dc.contributor.authorInitials El-Sabban, ME
dc.contributor.authorReprintAddress Talhouk, RS (reprint author), Amer Univ Beirut, Fac Arts and Sci, Dept Biol, POB 11-0236, Beirut, Lebanon.
dc.contributor.authorUniversity American University of Beirut
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dc.description.citedCount 1
dc.description.citedTotWOSCount 1
dc.description.citedWOSCount 1
dc.format.extentCount 13
dc.identifier.coden CCAEB
dc.identifier.pubmedID 22142338
dc.identifier.scopusID 83255176107
dc.relation.ispartOfISOAbbr Cell Commun. Adhes.
dc.relation.ispartOfIssue 5
dc.relation.ispartofPubTitle Cell Communication and Adhesion
dc.relation.ispartofPubTitleAbbr Cell Commun. Adhes.
dc.relation.ispartOfVolume 18
dc.source.ID WOS:000297855000002
dc.type.publication Journal
dc.subject.otherAuthKeyword β-casein
dc.subject.otherAuthKeyword GJIC
dc.subject.otherAuthKeyword OCT-1
dc.subject.otherAuthKeyword STAT5
dc.subject.otherChemCAS beta tubulin, 87090-36-6
dc.subject.otherChemCAS cyclic AMP, 60-92-4
dc.subject.otherChemCAS prolactin, 12585-34-1, 50647-00-2, 9002-62-4
dc.subject.otherChemCAS Caseins
dc.subject.otherChemCAS DNA Probes
dc.subject.otherChemCAS Jak2 protein, mouse,
dc.subject.otherChemCAS Janus Kinase 2,
dc.subject.otherChemCAS Octamer Transcription Factor-1
dc.subject.otherChemCAS Pou2f1 protein, mouse
dc.subject.otherChemCAS Prolactin, 9002-62-4
dc.subject.otherChemCAS STAT5 Transcription Factor
dc.subject.otherChemCAS Stat5a protein, mouse
dc.subject.otherIndex beta casein
dc.subject.otherIndex beta integrin
dc.subject.otherIndex beta tubulin
dc.subject.otherIndex cyclic AMP
dc.subject.otherIndex isoprotein
dc.subject.otherIndex lamin
dc.subject.otherIndex prolactin
dc.subject.otherIndex protein oct 1
dc.subject.otherIndex STAT5 protein
dc.subject.otherIndex transcription factor
dc.subject.otherIndex unclassified drug
dc.subject.otherIndex animal cell
dc.subject.otherIndex article
dc.subject.otherIndex breast epithelium
dc.subject.otherIndex cell culture
dc.subject.otherIndex controlled study
dc.subject.otherIndex DNA binding
dc.subject.otherIndex electrophoretic mobility
dc.subject.otherIndex gap junction
dc.subject.otherIndex gene translocation
dc.subject.otherIndex mouse
dc.subject.otherIndex nonhuman
dc.subject.otherIndex priority journal
dc.subject.otherIndex protein expression
dc.subject.otherIndex protein function
dc.subject.otherIndex protein phosphorylation
dc.subject.otherIndex receptor upregulation
dc.subject.otherIndex Western blotting
dc.subject.otherIndex Active Transport, Cell Nucleus
dc.subject.otherIndex Animals
dc.subject.otherIndex Caseins
dc.subject.otherIndex Cell Culture Techniques
dc.subject.otherIndex Cell Differentiation
dc.subject.otherIndex Cell Line
dc.subject.otherIndex DNA Probes
dc.subject.otherIndex Epithelial Cells
dc.subject.otherIndex Female
dc.subject.otherIndex Gap Junctions
dc.subject.otherIndex Gene Expression
dc.subject.otherIndex Gene Expression Regulation
dc.subject.otherIndex Janus Kinase 2
dc.subject.otherIndex Mammary Glands, Animal
dc.subject.otherIndex Mice
dc.subject.otherIndex Octamer Transcription Factor-1
dc.subject.otherIndex Phosphorylation
dc.subject.otherIndex Prolactin
dc.subject.otherIndex Protein Binding
dc.subject.otherIndex Receptor Cross-Talk
dc.subject.otherIndex Signal Transduction
dc.subject.otherIndex STAT5 Transcription Factor
dc.subject.otherKeywordPlus TRANSCRIPTION FACTOR OCT-1
dc.subject.otherKeywordPlus DNA-BINDING DOMAIN
dc.subject.otherKeywordPlus EXTRACELLULAR-MATRIX
dc.subject.otherKeywordPlus POU-DOMAIN
dc.subject.otherKeywordPlus GLAND DEVELOPMENT
dc.subject.otherKeywordPlus GENE PROMOTER
dc.subject.otherKeywordPlus BREAST-CANCER
dc.subject.otherKeywordPlus PSEUDOKINASE DOMAIN
dc.subject.otherWOS Biochemistry and Molecular Biology
dc.subject.otherWOS Cell Biology

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