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Kefir induces cell-cycle arrest and apoptosis in HTLV-l-negative malignant T-lymphocytes

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dc.contributor.author Maalouf K.
dc.contributor.author Baydoun E.
dc.contributor.author Rizk S.
dc.contributor.editor
dc.date 2011
dc.date.accessioned 2017-10-03T15:43:25Z
dc.date.available 2017-10-03T15:43:25Z
dc.date.issued 2011
dc.identifier 10.2147/CMR.S15109
dc.identifier.isbn
dc.identifier.issn 11791322
dc.identifier.uri http://hdl.handle.net/10938/12423
dc.description.abstract Background: Adult lymphoblastic leukemia (ALL) is a malignancy that occurs in white blood cells. The overall cure rate in children is 85percent, whereas it is only 40percent in adults. Kefir is an important probiotic that contains many bioactive ingredients, which give it unique health benefits. It has been shown to control several cellular types of cancer. Purpose: The present study investigates the effect of a cell-free fraction of kefir on CEM and Jurkat cells, which are human T-lymphotropic virus type I (HTLV-1)-negative malignant T-lymphocytes. Methods: Cells were incubated with different kefir concentrations. The cytotoxicity of the compound was evaluated by determining the percentage viability of cells. The effect of all the noncytotoxic concentrations of kefir on the proliferation of CEM and Jurkat cells was then assessed. The levels of transforming growth factor-alpha (TGF-α), transforming growth factor- beta1 (TGF-β1), matrix metalloproteinase-2 (MMP-2), and MMP-9 mRNA upon kefir treatment were then analyzed using reverse transcriptase polymerase chain reaction (RT-PCR). Finally, the growth inhibitory effects of kefir on cell-cycle progression-apoptosis were assessed by Cell Death Detection (ELISA) and flow cytometry. Results: The maximum cytotoxicity recorded after 48-hours treatment with 80 μg-μL kefir was only 42percent and 39percent in CEM and Jurkat cells, respectively. The percent reduction in proliferation was very significant, and was dose-, and time-dependent. In both cell lines, kefir exhibited its antiproliferative effect by downregulating TGF-α and upregulating TGFaβ 1 mRNA expression. Upon kefir treatment, a marked increase in cell-cycle distribution was noted in the preG1 phase of CEM and Jurkat cells, indicating the proapoptotic effect of kefir, which was further confirmed by Cell Death Detection ELISA. However, kefir did not affect the mRNA expression of metalloproteinases needed for the invasion of leukemic cell lines. Conclusion: In conclusion, kefir is effective in inhibiting proliferation and inducing apoptosis of HTLV-1-negative malignant T-lymphocytes. Therefore, further in vivo investigation is highly recommended. © 2011 Maalouf et al.
dc.format.extent
dc.format.extent Pages: (39-47)
dc.language English
dc.relation.ispartof Publication Name: Cancer Management and Research; Publication Year: 2011; Volume: 3; no. 1; Pages: (39-47);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other
dc.title Kefir induces cell-cycle arrest and apoptosis in HTLV-l-negative malignant T-lymphocytes
dc.type Article
dc.contributor.affiliation Maalouf, K., Department of Natural Sciences, Lebanese American University, Beirut, Lebanon
dc.contributor.affiliation Baydoun, E., Department of Biology, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Rizk, S., Department of Natural Sciences, Lebanese American University, Beirut, Lebanon
dc.contributor.authorAddress Rizk, S.; Department of Natural Sciences, Lebanese American University, P.O. Box 13-5053, Beirut 1102-2801, Lebanon; email: sandra.rizk@lau.edu.lb
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Arts and Sciences; Department: Biology;
dc.contributor.authorDepartment Biology
dc.contributor.authorDivision
dc.contributor.authorEmail
dc.contributor.authorFaculty Faculty of Arts and Sciences
dc.contributor.authorInitials
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut
dc.description.cited
dc.description.citedCount 4
dc.description.citedTotWOSCount
dc.description.citedWOSCount
dc.format.extentCount 9
dc.identifier.articleNo
dc.identifier.coden
dc.identifier.pubmedID
dc.identifier.scopusID 79952972804
dc.identifier.url
dc.publisher.address
dc.relation.ispartofConference
dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr
dc.relation.ispartOfIssue 1
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Cancer Management and Research
dc.relation.ispartofPubTitleAbbr Cancer Manage. Res.
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 3
dc.source.ID
dc.type.publication Journal
dc.subject.otherAuthKeyword Apoptosis
dc.subject.otherAuthKeyword Cancer
dc.subject.otherAuthKeyword CEM
dc.subject.otherAuthKeyword Jurkat
dc.subject.otherAuthKeyword Kefir
dc.subject.otherAuthKeyword Leukemia
dc.subject.otherChemCAS gelatinase A, 146480-35-5
dc.subject.otherChemCAS gelatinase B, 146480-36-6
dc.subject.otherIndex gelatinase A
dc.subject.otherIndex gelatinase B
dc.subject.otherIndex kefir
dc.subject.otherIndex messenger RNA
dc.subject.otherIndex transforming growth factor alpha
dc.subject.otherIndex antineoplastic activity
dc.subject.otherIndex apoptosis
dc.subject.otherIndex article
dc.subject.otherIndex cancer inhibition
dc.subject.otherIndex cell cycle arrest
dc.subject.otherIndex cell cycle G1 phase
dc.subject.otherIndex cell cycle progression
dc.subject.otherIndex cell cycle regulation
dc.subject.otherIndex cell death
dc.subject.otherIndex cell division
dc.subject.otherIndex cell free system
dc.subject.otherIndex cell proliferation
dc.subject.otherIndex cell viability
dc.subject.otherIndex child
dc.subject.otherIndex concentration response
dc.subject.otherIndex controlled study
dc.subject.otherIndex down regulation
dc.subject.otherIndex drug cytotoxicity
dc.subject.otherIndex drug effect
dc.subject.otherIndex drug efficacy
dc.subject.otherIndex drug mechanism
dc.subject.otherIndex enzyme linked immunosorbent assay
dc.subject.otherIndex female
dc.subject.otherIndex flow cytometry
dc.subject.otherIndex gene expression regulation
dc.subject.otherIndex growth inhibition
dc.subject.otherIndex human
dc.subject.otherIndex human cell
dc.subject.otherIndex Human T cell leukemia virus 1
dc.subject.otherIndex IC 50
dc.subject.otherIndex incubation time
dc.subject.otherIndex leukemia cell line
dc.subject.otherIndex preschool child
dc.subject.otherIndex reverse transcription polymerase chain reaction
dc.subject.otherIndex T cell leukemia
dc.subject.otherIndex upregulation
dc.subject.otherKeywordPlus
dc.subject.otherWOS


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