AUB ScholarWorks

Interleukin-1 β inhibits Na+-K+ATPase activity and protein expression in cardiac myocytes

Show simple item record Kreydiyyeh S.I. Abou-Chahine C. Hilal-Dandan R.
dc.contributor.editor 2004 2017-10-03T15:43:31Z 2017-10-03T15:43:31Z 2004
dc.identifier 10.1016/j.cyto.2003.11.014
dc.identifier.issn 10434666
dc.description.abstract Recent studies have shown that heart diseases are always accompanied with high levels of IL-1β and a decrease in Na+-K+ATPase concentrations. This work studies the involvement of the cytokine in the observed changes in the pump. Rats were injected intraperitoneally with 400mg of IL-1β and 4h later, the heart was isolated and a crude homogenate of the right and left ventricles was prepared and tested for Na+-K +ATPase activity and protein expression. IL-1β inhibited by around 70percent the activity of the ATPase in the left and right ventricles. This inhibition of the pump was ascribed to a decrease in its protein expression as demonstrated by western blot analysis. A dose and time response study conducted on isolated cardiac myocytes confirmed the inhibitory role of the cytokine on the ATPase and showed that IL-1β exerts its maximal down-regulatory effect at 2h and at a dose of 20ng-ml. The cytokine caused also an up-regulation of the NaKCl2 cotransporter. Both MEK and p38MAPK were shown to be involved in the signaling pathway activated by the cytokine. It can be concluded that the decrease in the Na+-K+ATPase concentration observed in heart diseases is a consequence of the accompanying high levels of IL-1β, and may be responsible for the different symptoms that accompany cardiac ischemia. © 2004 Elsevier Ltd. All rights reserved.
dc.format.extent Pages: (1-8)
dc.language English
dc.publisher LONDON
dc.relation.ispartof Publication Name: Cytokine; Publication Year: 2004; Volume: 26; no. 1; Pages: (1-8);
dc.source Scopus
dc.title Interleukin-1 β inhibits Na+-K+ATPase activity and protein expression in cardiac myocytes
dc.type Article
dc.contributor.affiliation Kreydiyyeh, S.I., Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Abou-Chahine, C., Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Hilal-Dandan, R., Department of Pharmacology, University of California, San Diego, CA, United States
dc.contributor.authorAddress Kreydiyyeh, S.I.; Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Beirut, Lebanon; email:
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Arts and Sciences; Department: Biology;
dc.contributor.authorDepartment Biology
dc.contributor.faculty Faculty of Arts and Sciences
dc.contributor.authorInitials Kreydiyyeh, SI
dc.contributor.authorInitials Abou-Chahine, C
dc.contributor.authorInitials Hilal-Dandan, R
dc.contributor.authorReprintAddress Kreydiyyeh, SI (reprint author), American Univ, Fac Arts and Sci, Dept Biol, Beirut, Lebanon.
dc.contributor.authorUniversity American University of Beirut
dc.description.cited ANDREASEN F, 1993, EUR J CLIN INVEST, V23, P234, DOI 10.1111-j.1365-2362.1993.tb00767.x; BIAGI RW, 1967, LANCET, V1, P849; Bick RJ, 1997, AM J PHYSIOL-HEART C, V272, pH1937; Bick RJ, 1999, J INTERF CYTOK RES, V19, P645, DOI 10.1089-107999099313794; BLANCO MP, 1999, AM J PHYSIOL-RENAL, V275, pF633; BRATER DC, 1992, EUR HEART J, V13, P10; Bundgaard Henning, 1996, Molecular and Cellular Biochemistry, V163-164, P277, DOI 10.1007-BF00408668; CLEMO HF, 1995, CIRC RES, V77, P741; Diaz RJ, 2003, J MOL CELL CARDIOL, V35, P45, DOI 10.1016-S0022-2828(02)00277-8; DIKSHIT K, 1973, NEW ENGL J MED, V288, P1087, DOI 10.1056-NEJM197305242882102; Eder J, 1997, TRENDS PHARMACOL SCI, V18, P319, DOI 10.1016-S0165-6147(97)01097-3; Hilal-Dandan R, 2000, J MOL CELL CARDIOL, V32, P1211, DOI 10.1006-jmcc.2000.1156; Hume JR, 2000, PHYSIOL REV, V80, P31; JIANG B, 2001, ARTERIOSCLER THROMB, V21, P19257; Karmazyn M, 1999, CIRC RES, V85, P777; KATO D, 1987, CIRC RES, V60, P185; Kelso E, 2000, EUR J PHARMACOL, V400, P43, DOI 10.1016-S0014-2999(00)00357-5; Kreydiyyeh SI, 2002, EUR CYTOKINE NETW, V13, P358; Kreydiyyeh SI, 2002, J INTERF CYTOK RES, V22, P1041, DOI 10.1089-107999002760624279; LIU S, 1989, MOL CELL BIOCHEM, V89, P147; Long C S, 2001, Heart Fail Rev, V6, P81; Mann DL, 2002, CIRC RES, V91, P988, DOI 10.1161-01.RES.0000043825.01705.1B; McDonough AA, 1996, AM J PHYSIOL-CELL PH, V270, pC1221; Ng DCH, 2001, J BIOL CHEM, V276, P29490, DOI 10.1074-jbc.M100699200; Niebauer J, 2000, INT J CARDIOL, V72, P209, DOI 10.1016-S0167-5273(99)00190-4; ONEILL WC, 1992, AM J PHYSIOL, V262, pC436; Paganelli F, 2001, CELL MOL BIOL, V47, P255; PARK CO, 1999, AM J PHYSIOL, V276, pH1590; Schwinger RHG, 1999, CIRCULATION, V99, P2105; Seta Y, 1996, J Card Fail, V2, P243, DOI 10.1016-S1071-9164(96)80047-9; SHAMRAJ OI, 1994, P NATL ACAD SCI USA, V91, P12952, DOI 10.1073-pnas.91.26.12952; SHAMRAJ OI, 1993, CARDIOVASC RES, V27, P2229, DOI 10.1093-cvr-27.12.2229; TAUSSKY HH, 1953, J BIOL CHEM, V202, P675; TAYLOR JE, 1993, PHILOSOPHIA, V22, P3, DOI 10.1007-BF02379804; TorreAmione G, 1996, CIRCULATION, V93, P704; YOKOYAMA T, 1993, J CLIN INVEST, V92, P2303, DOI 10.1172-JCI116834; Yokoyama T, 1997, CIRCULATION, V95, P1247
dc.description.citedCount 5
dc.description.citedTotWOSCount 5
dc.description.citedWOSCount 5
dc.format.extentCount 8
dc.identifier.coden CYTIE
dc.identifier.pubmedID 15016405
dc.identifier.scopusID 1542298179
dc.publisher.address 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND
dc.relation.ispartOfISOAbbr Cytokine
dc.relation.ispartOfIssue 1
dc.relation.ispartofPubTitle Cytokine
dc.relation.ispartofPubTitleAbbr Cytokine
dc.relation.ispartOfVolume 26
dc.source.ID WOS:000220635900001
dc.type.publication Journal
dc.subject.otherAuthKeyword Cardiomyocytes
dc.subject.otherAuthKeyword IL-1β
dc.subject.otherAuthKeyword MAPK
dc.subject.otherAuthKeyword Na+-K+ATPase
dc.subject.otherAuthKeyword NaKCl 2
dc.subject.otherChemCAS 2 (2 amino 3 methoxyphenyl)chromone, 167869-21-8
dc.subject.otherChemCAS 4 (4 fluorophenyl) 2 (4 hydroxyphenyl) 5 (4 pyridyl)imidazole, 152121-30-7
dc.subject.otherChemCAS protein, 67254-75-5
dc.subject.otherChemCAS Interleukin-1
dc.subject.otherChemCAS Mitogen-Activated Protein Kinases, EC
dc.subject.otherChemCAS Na(+)-K(+)-Exchanging ATPase, EC
dc.subject.otherChemCAS Sodium-Potassium-Chloride Symporters
dc.subject.otherChemCAS Tumor Necrosis Factor-alpha
dc.subject.otherIndex 2 (2 amino 3 methoxyphenyl)chromone
dc.subject.otherIndex 4 (4 fluorophenyl) 2 (4 hydroxyphenyl) 5 (4 pyridyl)imidazole
dc.subject.otherIndex adenosine triphosphatase (potassium sodium)
dc.subject.otherIndex interleukin 1beta
dc.subject.otherIndex mitogen activated protein kinase inhibitor
dc.subject.otherIndex mitogen activated protein kinase p38
dc.subject.otherIndex protein
dc.subject.otherIndex sodium potassium chloride cotransporter
dc.subject.otherIndex animal cell
dc.subject.otherIndex article
dc.subject.otherIndex controlled study
dc.subject.otherIndex enzyme activity
dc.subject.otherIndex heart disease
dc.subject.otherIndex heart left ventricle
dc.subject.otherIndex heart muscle cell
dc.subject.otherIndex heart right ventricle
dc.subject.otherIndex homogenate
dc.subject.otherIndex isolated heart
dc.subject.otherIndex nonhuman
dc.subject.otherIndex priority journal
dc.subject.otherIndex protein expression
dc.subject.otherIndex rat
dc.subject.otherIndex signal transduction
dc.subject.otherIndex Western blotting
dc.subject.otherIndex Animals
dc.subject.otherIndex Dose-Response Relationship, Drug
dc.subject.otherIndex Down-Regulation
dc.subject.otherIndex Heart Ventricles
dc.subject.otherIndex Interleukin-1
dc.subject.otherIndex Male
dc.subject.otherIndex MAP Kinase Signaling System
dc.subject.otherIndex Mitogen-Activated Protein Kinases
dc.subject.otherIndex Myocytes, Cardiac
dc.subject.otherIndex Na(+)-K(+)-Exchanging ATPase
dc.subject.otherIndex Rats
dc.subject.otherIndex Rats, Sprague-Dawley
dc.subject.otherIndex Sodium-Potassium-Chloride Symporters
dc.subject.otherIndex Tumor Necrosis Factor-alpha
dc.subject.otherIndex Up-Regulation
dc.subject.otherKeywordPlus NECROSIS-FACTOR-ALPHA
dc.subject.otherKeywordPlus HEART-FAILURE
dc.subject.otherKeywordPlus CELL-VOLUME
dc.subject.otherKeywordPlus VENTRICULAR MYOCYTES
dc.subject.otherKeywordPlus PUMP
dc.subject.otherKeywordPlus RAT
dc.subject.otherKeywordPlus COTRANSPORT
dc.subject.otherKeywordPlus MECHANISM
dc.subject.otherKeywordPlus ISOFORM
dc.subject.otherKeywordPlus DISEASE
dc.subject.otherWOS Biochemistry and Molecular Biology
dc.subject.otherWOS Cell Biology
dc.subject.otherWOS Immunology

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search AUB ScholarWorks


My Account