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In vitro cytotoxicity and mutagenicity of mainstream waterpipe smoke and its functional consequences on alveolar type II derived cells

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dc.contributor.author Rammah M.
dc.contributor.author Dandachi F.
dc.contributor.author Salman R.
dc.contributor.author Shihadeh A.
dc.contributor.author El-Sabban M.
dc.contributor.editor
dc.date Jun-2012
dc.date.accessioned 2017-10-04T11:15:51Z
dc.date.available 2017-10-04T11:15:51Z
dc.date.issued 2012
dc.identifier 10.1016/j.toxlet.2012.04.003
dc.identifier.isbn
dc.identifier.issn 03784274
dc.identifier.uri http://hdl.handle.net/10938/14870
dc.description.abstract Introduction: While waterpipe tobacco smoking has become a global phenomenon, its potential health consequences are poorly understood. In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). Methods: The mainstream WSC was generated using a standard laboratory machine protocol. We assessed its mutagenicity using Ames test. In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. Results: Within the range of tested doses, WSC did not elicit sufficient response to be considered mutagenic in any of the strains tested (TA98, TA100, TA102, and TA97a) but were found to be toxic for strains TA97a and TA102 at the highest tested doses. However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. Also our study indicated that WSC induced an increase in the transcriptional expression of matrix metalloproteinases, MMP-2 and MMP-9 and an immune response regulator, Toll Like Receptor-4. Conclusion: The data reported here represent the first in vitro demonstration of the effect of waterpipe smoke on cellular parameters providing evidence of the potential involvement of WPS in the pathogenesis of COPD through impairing cellular growth and inducing inflammation. © 2012 Elsevier Ireland Ltd.
dc.format.extent
dc.format.extent Pages: (220-231)
dc.language English
dc.publisher CLARE
dc.relation.ispartof Publication Name: Toxicology Letters; Publication Year: 2012; Volume: 211; no. 3; Pages: (220-231);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other
dc.title In vitro cytotoxicity and mutagenicity of mainstream waterpipe smoke and its functional consequences on alveolar type II derived cells
dc.type Article
dc.contributor.affiliation Rammah, M., Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Dandachi, F., Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Salman, R., Department of Mechanical Engineering, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Shihadeh, A., Department of Mechanical Engineering, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.affiliation El-Sabban, M., Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.authorAddress El-Sabban, M.; Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Bliss Street, P.O. Box 11-0236, Riad El-Solh 1107 2020, Beirut, Lebanon; email: me00@aub.edu.lb
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Engineering and Architecture; Department: Mechanical Engineering;
dc.contributor.authorDepartment Mechanical Engineering
dc.contributor.authorDivision
dc.contributor.authorEmail mr37@aub.edu.lb; farandandashi@yahoo.com; rs107@aub.edu.lb; as20@aub.edu.lb; me00@aub.edu.lb
dc.contributor.authorFaculty Faculty of Engineering and Architecture
dc.contributor.authorInitials Rammah, M
dc.contributor.authorInitials Dandachi, F
dc.contributor.authorInitials Salman, R
dc.contributor.authorInitials Shihadeh, A
dc.contributor.authorInitials El-Sabban, M
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress El-Sabban, M (reprint author), Amer Univ Beirut, Dept Anat Cell Biol and Physiol Sci, Fac Med, Bliss St,POB 11-0236, Beirut 11072020, Lebanon.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut
dc.description.cited Abbas T, 2009, NAT REV CANCER, V9, P400, DOI 10.1038-nrc2657; Akl EA, 2010, INT J EPIDEMIOL, V39, P834, DOI 10.1093-ije-dyq002; Al Rashidi M, 2008, FOOD CHEM TOXICOL, V46, P3546, DOI 10.1016-j.fct.2008.09.007; AMES BN, 1975, MUTAT RES, V31, P347, DOI 10.1016-0165-1161(75)90046-1; Azab M, 2010, NICOTINE TOB RES, V12, P606, DOI 10.1093-ntr-ntq055; Basu S, 2004, AM J PHYSIOL-LUNG C, V286, pL887, DOI 10.1152-ajplung.00323.2003; Bazarbachi A, 1999, BLOOD, V93, P278; Beausejour CM, 2003, EMBO J, V22, P4212, DOI 10.1093-emboj-cdg417; Bombick DW, 1997, FUND APPL TOXICOL, V39, P11, DOI 10.1006-faat.1997.2334; Combrink A, 2010, SAMJ S AFR MED J, V100, P297; Corbel M, 2000, BRAZ J MED BIOL RES, V33, P749, DOI 10.1590-S0100-879X2000000700004; Dar-Odeh NS, 2010, HARM REDUCT J, V7, DOI 10.1186-1477-7517-7-10; DiMauro T, 2009, AGING-US, V1, P182; DOOLITTLE DJ, 1990, MUTAT RES, V240, P59, DOI 10.1016-0165-1218(90)90008-P; Dugas E, 2010, PEDIATRICS, V125, P1184, DOI 10.1542-peds.2009-2335; El-Setouhy M, 2008, MUTAT RES-GEN TOX EN, V655, P36, DOI 10.1016-j.mrgentox.2008.06.014; Forrester K, 1996, P NATL ACAD SCI USA, V93, P2442, DOI 10.1073-pnas.93.6.2442; Hendzel MJ, 1997, CHROMOSOMA, V106, P348, DOI 10.1007-s004120050256; Jacob P., 2011, CANC EPIDEMIOLOGY BI; Johnston LD, 2010, MONITORING FUTURE NA; Juan G, 1998, CYTOMETRY, V32, P71, DOI 10.1002-(SICI)1097-0320(19980601)32:271::AID-CYTO13.0.CO;2-H; Katurji M, 2010, INHAL TOXICOL, V22, P1101, DOI 10.3109-08958378.2010.524265; Khabour OF, 2011, ENVIRON MOL MUTAGEN, V52, DOI 10.1002-em.20601; Kluchova Z, 2006, BIOLOGIA, V61, P643, DOI 10.2478-s11756-006-0135-4; Louhelainen Noora, 2008, Int J Chron Obstruct Pulmon Dis, V3, P585; MARON DM, 1983, MUTAT RES, V113, P173, DOI 10.1016-0165-1161(83)90010-9; Maziak W, 2011, ADDICT BEHAV, V36, P1, DOI 10.1016-j.addbeh.2010.08.030; Mendoza MGA, 2008, NEPHROLOGY, V13, P218, DOI 10.1111-j.1440-1797.2007.00897.x; Monzer B, 2008, FOOD CHEM TOXICOL, V46, P2991, DOI 10.1016-j.fct.2008.05.031; Nelson BV, 1997, J PEDIATR, V130, P423, DOI 10.1016-S0022-3476(97)70204-X; Ohbayashi H, 2002, CURR PROTEIN PEPT SC, V3, P409, DOI 10.2174-1389203023380549; Pace E, 2011, HUM IMMUNOL, V72, P54, DOI 10.1016-j.humimm.2010.09.009; PAGANO DA, 1987, MUTAT RES, V179, P159, DOI 10.1016-0027-5107(87)90306-X; Raad D, 2011, CHEST, V139, P764, DOI 10.1378-chest.10-0991; Ren JL, 2009, CELL SIGNAL, V21, P378, DOI 10.1016-j.cellsig.2008.10.011; Schubert J, 2011, TOXICOL LETT, V205, P279, DOI 10.1016-j.toxlet.2011.06.017; Segura-Valdez L, 2000, CHEST, V117, P684, DOI 10.1378-chest.117.3.684; Sepetdjian E, 2008, FOOD CHEM TOXICOL, V46, P1582, DOI 10.1016-j.fct.2007.12.028; Shihadeh A, 2003, FOOD CHEM TOXICOL, V41, P143, DOI 10.1016-S0278-6915(02)00220-X; Shihadeh A, 2005, FOOD CHEM TOXICOL, V43, P655, DOI 10.1016-j.fct.2004.12.013; SMITH BT, 1977, AM REV RESPIR DIS, V115, P285; Taha AZ, 2010, ANN SAUDI MED, V30, P222, DOI 10.4103-0256-4947.62838; Thorley Andrew J, 2007, Int J Chron Obstruct Pulmon Dis, V2, P409; Waked Mirna, 2011, Clin Epidemiol, V3, P315, DOI 10.2147-CLEP.S26350; White JL, 2001, FOOD CHEM TOXICOL, V39, P499, DOI 10.1016-S0278-6915(00)00155-1; Yadav JS, 2000, CYTOBIOS, V101, P101; YAHAGI T, 1975, CANCER LETT, V1, P91, DOI 10.1016-S0304-3835(75)95563-9; Yoshida T, 2007, PHYSIOL REV, V87, P1047, DOI 10.1152-physrev.00048.2006
dc.description.citedCount 8
dc.description.citedTotWOSCount 10
dc.description.citedWOSCount 10
dc.format.extentCount 12
dc.identifier.articleNo
dc.identifier.coden TOLED
dc.identifier.pubmedID 22516759
dc.identifier.scopusID 84860214458
dc.identifier.url
dc.publisher.address ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND
dc.relation.ispartofConference
dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr Toxicol. Lett.
dc.relation.ispartOfIssue 3
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Toxicology Letters
dc.relation.ispartofPubTitleAbbr Toxicol. Lett.
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 211
dc.source.ID WOS:000305496800002
dc.type.publication Journal
dc.subject.otherAuthKeyword Alveolar lung cells
dc.subject.otherAuthKeyword COPD
dc.subject.otherAuthKeyword Cytotoxicity
dc.subject.otherAuthKeyword Inflammation
dc.subject.otherAuthKeyword Mutagenicity
dc.subject.otherAuthKeyword Waterpipe smoke
dc.subject.otherChemCAS carbon monoxide, 630-08-0
dc.subject.otherChemCAS gelatinase A, 146480-35-5
dc.subject.otherChemCAS gelatinase B, 146480-36-6
dc.subject.otherChemCAS protein p21, 85306-28-1
dc.subject.otherChemCAS toll like receptor 4, 203811-83-0
dc.subject.otherChemCAS Matrix Metalloproteinases, 3.4.24.-
dc.subject.otherChemCAS Mutagens
dc.subject.otherChemCAS Nitric Oxide, 10102-43-9
dc.subject.otherChemCAS Smoke
dc.subject.otherChemCAS Toll-Like Receptor 4
dc.subject.otherIndex carbon monoxide
dc.subject.otherIndex cigarette smoke condensate
dc.subject.otherIndex gelatinase A
dc.subject.otherIndex gelatinase B
dc.subject.otherIndex protein p21
dc.subject.otherIndex protein p53
dc.subject.otherIndex toll like receptor 4
dc.subject.otherIndex Ames test
dc.subject.otherIndex article
dc.subject.otherIndex cell aging
dc.subject.otherIndex cell cycle arrest
dc.subject.otherIndex cell proliferation
dc.subject.otherIndex cell type
dc.subject.otherIndex chronic obstructive lung disease
dc.subject.otherIndex cytotoxicity
dc.subject.otherIndex gene expression
dc.subject.otherIndex human
dc.subject.otherIndex human cell
dc.subject.otherIndex human cell culture
dc.subject.otherIndex in vitro study
dc.subject.otherIndex inflammation
dc.subject.otherIndex lung alveolus cell type 2
dc.subject.otherIndex mainstream waterpipe smoke
dc.subject.otherIndex mutagenicity
dc.subject.otherIndex pathophysiology
dc.subject.otherIndex priority journal
dc.subject.otherIndex protein expression
dc.subject.otherIndex smoke
dc.subject.otherIndex vascular endothelial cell
dc.subject.otherIndex Animals
dc.subject.otherIndex Apoptosis
dc.subject.otherIndex Blotting, Western
dc.subject.otherIndex Cell Aging
dc.subject.otherIndex Cell Cycle
dc.subject.otherIndex Cell Line, Tumor
dc.subject.otherIndex Cell Nucleus
dc.subject.otherIndex Cell Proliferation
dc.subject.otherIndex Cell Survival
dc.subject.otherIndex Electric Impedance
dc.subject.otherIndex Endothelial Cells
dc.subject.otherIndex Epithelial Cells
dc.subject.otherIndex Flow Cytometry
dc.subject.otherIndex Fluorescent Antibody Technique
dc.subject.otherIndex Humans
dc.subject.otherIndex Matrix Metalloproteinases
dc.subject.otherIndex Mutagenicity Tests
dc.subject.otherIndex Mutagens
dc.subject.otherIndex Nitric Oxide
dc.subject.otherIndex Pulmonary Alveoli
dc.subject.otherIndex Rats
dc.subject.otherIndex Rats, Sprague-Dawley
dc.subject.otherIndex Real-Time Polymerase Chain Reaction
dc.subject.otherIndex Smoke
dc.subject.otherIndex Tobacco
dc.subject.otherIndex Toll-Like Receptor 4
dc.subject.otherIndex Nicotiana tabacum
dc.subject.otherKeywordPlus POLYCYCLIC AROMATIC-HYDROCARBONS
dc.subject.otherKeywordPlus NARGHILE WATERPIPE
dc.subject.otherKeywordPlus NITRIC-OXIDE
dc.subject.otherKeywordPlus PIPE SMOKING
dc.subject.otherKeywordPlus UNIVERSITY-STUDENTS
dc.subject.otherKeywordPlus CELLULAR SENESCENCE
dc.subject.otherKeywordPlus TOBACCO SMOKING
dc.subject.otherKeywordPlus CIGARETTE-SMOKE
dc.subject.otherKeywordPlus LUNG INJURY
dc.subject.otherKeywordPlus PHOSPHORYLATION
dc.subject.otherWOS Toxicology


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