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In vitro cytotoxicity and mutagenicity of mainstream waterpipe smoke and its functional consequences on alveolar type II derived cells

Show simple item record Rammah M. Dandachi F. Salman R. Shihadeh A. El-Sabban M.
dc.contributor.editor Jun-2012 2017-10-04T11:15:51Z 2017-10-04T11:15:51Z 2012
dc.identifier 10.1016/j.toxlet.2012.04.003
dc.identifier.issn 03784274
dc.description.abstract Introduction: While waterpipe tobacco smoking has become a global phenomenon, its potential health consequences are poorly understood. In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). Methods: The mainstream WSC was generated using a standard laboratory machine protocol. We assessed its mutagenicity using Ames test. In addition, we studied the effect of WSC on the proliferation and cell cycle of alveolar type II cells and vascular endothelial cells. We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. Results: Within the range of tested doses, WSC did not elicit sufficient response to be considered mutagenic in any of the strains tested (TA98, TA100, TA102, and TA97a) but were found to be toxic for strains TA97a and TA102 at the highest tested doses. However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. Also our study indicated that WSC induced an increase in the transcriptional expression of matrix metalloproteinases, MMP-2 and MMP-9 and an immune response regulator, Toll Like Receptor-4. Conclusion: The data reported here represent the first in vitro demonstration of the effect of waterpipe smoke on cellular parameters providing evidence of the potential involvement of WPS in the pathogenesis of COPD through impairing cellular growth and inducing inflammation. © 2012 Elsevier Ireland Ltd.
dc.format.extent Pages: (220-231)
dc.language English
dc.publisher CLARE
dc.relation.ispartof Publication Name: Toxicology Letters; Publication Year: 2012; Volume: 211; no. 3; Pages: (220-231);
dc.source Scopus
dc.title In vitro cytotoxicity and mutagenicity of mainstream waterpipe smoke and its functional consequences on alveolar type II derived cells
dc.type Article
dc.contributor.affiliation Rammah, M., Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Dandachi, F., Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Salman, R., Department of Mechanical Engineering, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Shihadeh, A., Department of Mechanical Engineering, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.affiliation El-Sabban, M., Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.authorAddress El-Sabban, M.; Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Bliss Street, P.O. Box 11-0236, Riad El-Solh 1107 2020, Beirut, Lebanon; email:
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Engineering and Architecture; Department: Mechanical Engineering;
dc.contributor.authorDepartment Mechanical Engineering
dc.contributor.authorFaculty Faculty of Engineering and Architecture
dc.contributor.authorInitials Rammah, M
dc.contributor.authorInitials Dandachi, F
dc.contributor.authorInitials Salman, R
dc.contributor.authorInitials Shihadeh, A
dc.contributor.authorInitials El-Sabban, M
dc.contributor.authorReprintAddress El-Sabban, M (reprint author), Amer Univ Beirut, Dept Anat Cell Biol and Physiol Sci, Fac Med, Bliss St,POB 11-0236, Beirut 11072020, Lebanon.
dc.contributor.authorUniversity American University of Beirut
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dc.description.citedCount 8
dc.description.citedTotWOSCount 10
dc.description.citedWOSCount 10
dc.format.extentCount 12
dc.identifier.coden TOLED
dc.identifier.pubmedID 22516759
dc.identifier.scopusID 84860214458
dc.relation.ispartOfISOAbbr Toxicol. Lett.
dc.relation.ispartOfIssue 3
dc.relation.ispartofPubTitle Toxicology Letters
dc.relation.ispartofPubTitleAbbr Toxicol. Lett.
dc.relation.ispartOfVolume 211
dc.source.ID WOS:000305496800002
dc.type.publication Journal
dc.subject.otherAuthKeyword Alveolar lung cells
dc.subject.otherAuthKeyword COPD
dc.subject.otherAuthKeyword Cytotoxicity
dc.subject.otherAuthKeyword Inflammation
dc.subject.otherAuthKeyword Mutagenicity
dc.subject.otherAuthKeyword Waterpipe smoke
dc.subject.otherChemCAS carbon monoxide, 630-08-0
dc.subject.otherChemCAS gelatinase A, 146480-35-5
dc.subject.otherChemCAS gelatinase B, 146480-36-6
dc.subject.otherChemCAS protein p21, 85306-28-1
dc.subject.otherChemCAS toll like receptor 4, 203811-83-0
dc.subject.otherChemCAS Matrix Metalloproteinases, 3.4.24.-
dc.subject.otherChemCAS Mutagens
dc.subject.otherChemCAS Nitric Oxide, 10102-43-9
dc.subject.otherChemCAS Smoke
dc.subject.otherChemCAS Toll-Like Receptor 4
dc.subject.otherIndex carbon monoxide
dc.subject.otherIndex cigarette smoke condensate
dc.subject.otherIndex gelatinase A
dc.subject.otherIndex gelatinase B
dc.subject.otherIndex protein p21
dc.subject.otherIndex protein p53
dc.subject.otherIndex toll like receptor 4
dc.subject.otherIndex Ames test
dc.subject.otherIndex article
dc.subject.otherIndex cell aging
dc.subject.otherIndex cell cycle arrest
dc.subject.otherIndex cell proliferation
dc.subject.otherIndex cell type
dc.subject.otherIndex chronic obstructive lung disease
dc.subject.otherIndex cytotoxicity
dc.subject.otherIndex gene expression
dc.subject.otherIndex human
dc.subject.otherIndex human cell
dc.subject.otherIndex human cell culture
dc.subject.otherIndex in vitro study
dc.subject.otherIndex inflammation
dc.subject.otherIndex lung alveolus cell type 2
dc.subject.otherIndex mainstream waterpipe smoke
dc.subject.otherIndex mutagenicity
dc.subject.otherIndex pathophysiology
dc.subject.otherIndex priority journal
dc.subject.otherIndex protein expression
dc.subject.otherIndex smoke
dc.subject.otherIndex vascular endothelial cell
dc.subject.otherIndex Animals
dc.subject.otherIndex Apoptosis
dc.subject.otherIndex Blotting, Western
dc.subject.otherIndex Cell Aging
dc.subject.otherIndex Cell Cycle
dc.subject.otherIndex Cell Line, Tumor
dc.subject.otherIndex Cell Nucleus
dc.subject.otherIndex Cell Proliferation
dc.subject.otherIndex Cell Survival
dc.subject.otherIndex Electric Impedance
dc.subject.otherIndex Endothelial Cells
dc.subject.otherIndex Epithelial Cells
dc.subject.otherIndex Flow Cytometry
dc.subject.otherIndex Fluorescent Antibody Technique
dc.subject.otherIndex Humans
dc.subject.otherIndex Matrix Metalloproteinases
dc.subject.otherIndex Mutagenicity Tests
dc.subject.otherIndex Mutagens
dc.subject.otherIndex Nitric Oxide
dc.subject.otherIndex Pulmonary Alveoli
dc.subject.otherIndex Rats
dc.subject.otherIndex Rats, Sprague-Dawley
dc.subject.otherIndex Real-Time Polymerase Chain Reaction
dc.subject.otherIndex Smoke
dc.subject.otherIndex Tobacco
dc.subject.otherIndex Toll-Like Receptor 4
dc.subject.otherIndex Nicotiana tabacum
dc.subject.otherKeywordPlus NARGHILE WATERPIPE
dc.subject.otherKeywordPlus NITRIC-OXIDE
dc.subject.otherKeywordPlus PIPE SMOKING
dc.subject.otherKeywordPlus UNIVERSITY-STUDENTS
dc.subject.otherKeywordPlus CELLULAR SENESCENCE
dc.subject.otherKeywordPlus TOBACCO SMOKING
dc.subject.otherKeywordPlus CIGARETTE-SMOKE
dc.subject.otherKeywordPlus LUNG INJURY
dc.subject.otherKeywordPlus PHOSPHORYLATION
dc.subject.otherWOS Toxicology

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