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Impact of embryonic passaging of H9N2 virus on pathogenicity and stability of HA1- amino acid sequence cleavage site

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dc.contributor.author Shaib H.A.
dc.contributor.author Cochet N.
dc.contributor.author Ribeiro T.
dc.contributor.author Abdel Nour A.M.
dc.contributor.author Nemer G.
dc.contributor.author Barbour E.K.
dc.contributor.editor
dc.date 2010
dc.date.accessioned 2017-10-05T15:30:55Z
dc.date.available 2017-10-05T15:30:55Z
dc.date.issued 2010
dc.identifier
dc.identifier.isbn
dc.identifier.issn 15076164
dc.identifier.uri http://hdl.handle.net/10938/15280
dc.description.abstract Background: Highly virulent Avian Influenza viruses might arise from avirulent strains following viral passaging. This work aims at studying the impact of embryonic passaging of H9N2 on the stability of the HA1 amino acid sequence and its relatedness to pathogenicity. Material-Methods: The original H9N2 virus was propagated for 3 consecutive passages in embryonated chicken eggs. Pathogenicity and amino acids sequences at the HA1 gene level of the original (P0), and the once (P1), twice (P2), and three times (P3) passaged viruses were compared. Results: The percent mortality significantly increased in embryos inoculated with P2 (86.7percent) and P3 of H9N2 (100percent) in comparison to P0 (0.0percent) and P1 of H9N2 (46.1percent) (P0.05), while the density of propagated H9N2 declined with passaging. The R-S-S-R motif was stable at the HA1 cleavage site of P0, P1, P2, and P3 viruses. The similarity in the HA1 sequences among the differently passaged viruses ranged between 93.2 to 100percent. Conclusions: The pathogenicity increased significantly upon passaging in chicken embryos in spite of the presence of the same motif at the HA1 cleavage site. Further investigations will target the study of changes in the whole HA protein and of Neuraminidases that could be responsible for a higher pathogenicity. © Med Sci Monit, 2010.
dc.format.extent
dc.language English
dc.relation.ispartof Publication Name: Case Reports and Clinical Practice Review; Publication Year: 2010; Volume: 16; no. 10;
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other
dc.title Impact of embryonic passaging of H9N2 virus on pathogenicity and stability of HA1- amino acid sequence cleavage site
dc.type Article
dc.contributor.affiliation Shaib, H.A., Department of Animal and Veterinary Sciences, Faculty of Agricultural and Food Sciences, American University of Beirut (AUB), Beirut, Lebanon
dc.contributor.affiliation Cochet, N., Department of Biological Engineering, Université de Technologie de Compiègne, Compiègne, France
dc.contributor.affiliation Ribeiro, T., Institut Polytechnique LaSalle Beauvais, Beauvais, France
dc.contributor.affiliation Abdel Nour, A.M., Institut Polytechnique LaSalle Beauvais, Beauvais, France
dc.contributor.affiliation Nemer, G., Department of Biochemistry, Faculty of Medicine, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Barbour, E.K., Department of Animal and Veterinary Sciences, Faculty of Agricultural and Food Sciences, American University of Beirut (AUB), Beirut, Lebanon
dc.contributor.authorAddress Barbour, E. K.; Department of Animal and Veterinary Sciences, Faculty of Agricultural and Food Sciences, American University of Beirut (AUB), P.O. Box 11-0236, Beirut, Lebanon; email: eb01@aub.edu.lb
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Biochemistry and Molecular Genetics;
dc.contributor.authorDepartment Biochemistry and Molecular Genetics
dc.contributor.authorDivision
dc.contributor.authorEmail
dc.contributor.authorFaculty Faculty of Medicine
dc.contributor.authorInitials empty
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut Medical Center
dc.description.cited
dc.description.citedCount 3
dc.description.citedTotWOSCount
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dc.identifier.articleNo
dc.identifier.coden
dc.identifier.pubmedID
dc.identifier.scopusID 78049290019
dc.identifier.url
dc.publisher.address
dc.relation.ispartofConference
dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr
dc.relation.ispartOfIssue 10
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Case Reports and Clinical Practice Review
dc.relation.ispartofPubTitleAbbr Case Rep. Clin. Pract. Rev.
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 16
dc.source.ID
dc.type.publication Journal
dc.subject.otherAuthKeyword Amino acid sequence
dc.subject.otherAuthKeyword Chicken embryo
dc.subject.otherAuthKeyword HA1 protein
dc.subject.otherAuthKeyword Influenza H9N2 virus
dc.subject.otherAuthKeyword Passages
dc.subject.otherAuthKeyword Pathogenicity
dc.subject.otherChemCAS hemagglutinin, 37333-12-3
dc.subject.otherChemCAS sialidase, 9001-67-6
dc.subject.otherIndex hemagglutinin
dc.subject.otherIndex hemagglutinin 1
dc.subject.otherIndex sialidase
dc.subject.otherIndex unclassified drug
dc.subject.otherIndex amino acid sequence
dc.subject.otherIndex article
dc.subject.otherIndex chick embryo
dc.subject.otherIndex controlled study
dc.subject.otherIndex embryo
dc.subject.otherIndex Influenza virus A H9N2
dc.subject.otherIndex nonhuman
dc.subject.otherIndex protein cleavage
dc.subject.otherIndex protein motif
dc.subject.otherIndex protein stability
dc.subject.otherIndex reverse transcription polymerase chain reaction
dc.subject.otherIndex sequence analysis
dc.subject.otherIndex virus replication
dc.subject.otherIndex virus virulence
dc.subject.otherKeywordPlus
dc.subject.otherWOS


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