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Effects of subinhibitory concentrations of antimicrobial agents on escherichia coli O157:H7 Shiga toxin release and role of the SOS response

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dc.contributor.author Nassar F.J.
dc.contributor.author Rahal E.A.
dc.contributor.author Sabra A.
dc.contributor.author Matar G.M.
dc.contributor.editor
dc.date 2013
dc.date.accessioned 2017-10-05T15:35:29Z
dc.date.available 2017-10-05T15:35:29Z
dc.date.issued 2013
dc.identifier 10.1089/fpd.2013.1510
dc.identifier.isbn
dc.identifier.issn 15353141
dc.identifier.uri http://hdl.handle.net/10938/15505
dc.description.abstract Treatment of Escherichia coli O157:H7 by certain antimicrobial agents often exacerbates the patient's condition by increasing either the release of preformed Shiga toxins (Stx) upon cell lysis or their production through the SOS response-triggered induction of Stx-producing prophages. Recommended subinhibitory concentrations (sub-MICs) of azithromycin (AZI), gentamicin (GEN), imipenem (IMI), and rifampicin (RIF) were evaluated in comparison to norfloxacin (NOR), an SOS-inducer, to assess the role of the SOS response in Stx release. Relative expression of recA (SOS-inducer), Q (late antitermination gene of Stx-producing prophage), stx1, and stx2 genes was assessed at two sub-MICs of the antimicrobials for two different strains of E. coli O157:H7 using reverse transcription-real-time polymerase chain reaction. Both strains at the two sub-MICs were also subjected to Western blotting for LexA protein expression and to reverse passive latex agglutination for Stx detection. For both strains at both sub-MICs, NOR and AZI caused SOS-induced Stx production (high recA, Q, and stx2 gene expression and high Stx2 production), so they should be avoided in E. coli O157:H7 treatment; however, sub-MICs of RIF and IMI induced Stx2 production in an SOS-independent manner except for one strain at the first twofold dilution below MIC of RIF where Stx2 production decreased. Moreover, GEN caused somewhat increased Stx2 production due to its mode of action rather than any effect on gene expression. The choice of antimicrobial therapy should rely on the antimicrobial mode of action, its concentration, and on the nature of the strain. © 2013, Mary Ann Liebert, Inc.
dc.format.extent
dc.format.extent Pages: (805-812)
dc.language English
dc.publisher NEW ROCHELLE
dc.relation.ispartof Publication Name: Foodborne Pathogens and Disease; Publication Year: 2013; Volume: 10; no. 9; Pages: (805-812);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other
dc.title Effects of subinhibitory concentrations of antimicrobial agents on escherichia coli O157:H7 Shiga toxin release and role of the SOS response
dc.type Article
dc.contributor.affiliation Nassar, F.J., Department of Experimental Pathology Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 3 Dag Hammarskjold Plaza, New York, NY 10017, United States
dc.contributor.affiliation Rahal, E.A., Department of Experimental Pathology Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 3 Dag Hammarskjold Plaza, New York, NY 10017, United States
dc.contributor.affiliation Sabra, A., Department of Experimental Pathology Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 3 Dag Hammarskjold Plaza, New York, NY 10017, United States
dc.contributor.affiliation Matar, G.M., Department of Experimental Pathology Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 3 Dag Hammarskjold Plaza, New York, NY 10017, United States
dc.contributor.authorAddress Matar, G.M.; Department of Experimental Pathology Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 3 Dag Hammarskjold Plaza, New York, NY 10017, United States; email: gmatar@aub.edu.lb
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Experimental Pathology, Immunology and Microbiology;
dc.contributor.authorDepartment Experimental Pathology, Immunology and Microbiology
dc.contributor.authorDivision
dc.contributor.authorEmail gmatar@aub.edu.lb
dc.contributor.authorFaculty Faculty of Medicine
dc.contributor.authorInitials Nassar, FJ
dc.contributor.authorInitials Rahal, EA
dc.contributor.authorInitials Sabra, A
dc.contributor.authorInitials Matar, GM
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Matar, GM (reprint author), Amer Univ Beirut, Fac Med, Dept Expt Pathol Immunol and Microbiol, 3 Dag Hammarskjold Plaza,8th Flr, New York, NY 10017 USA.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut Medical Center
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dc.description.citedCount 1
dc.description.citedTotWOSCount 4
dc.description.citedWOSCount 3
dc.format.extentCount 8
dc.identifier.articleNo
dc.identifier.coden
dc.identifier.pubmedID 23808851
dc.identifier.scopusID 84883379382
dc.identifier.url
dc.publisher.address 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA
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dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr Foodborne Pathog. Dis.
dc.relation.ispartOfIssue 9
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Foodborne Pathogens and Disease
dc.relation.ispartofPubTitleAbbr Foodborne Pathog. Dis.
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 10
dc.source.ID WOS:000330456900009
dc.type.publication Journal
dc.subject.otherAuthKeyword
dc.subject.otherChemCAS Shiga toxin, 75757-64-1
dc.subject.otherChemCAS azithromycin, 83905-01-5
dc.subject.otherChemCAS gentamicin, 1392-48-9, 1403-66-3, 1405-41-0
dc.subject.otherChemCAS imipenem, 64221-86-9
dc.subject.otherChemCAS norfloxacin, 70458-96-7
dc.subject.otherChemCAS rifampicin, 13292-46-1
dc.subject.otherIndex antiinfective agent
dc.subject.otherIndex azithromycin
dc.subject.otherIndex gentamicin
dc.subject.otherIndex imipenem
dc.subject.otherIndex LexA protein
dc.subject.otherIndex norfloxacin
dc.subject.otherIndex rifampicin
dc.subject.otherIndex Shiga toxin
dc.subject.otherIndex antimicrobial therapy
dc.subject.otherIndex article
dc.subject.otherIndex concentration response
dc.subject.otherIndex controlled study
dc.subject.otherIndex DNA repair
dc.subject.otherIndex DNA synthesis
dc.subject.otherIndex energy yield
dc.subject.otherIndex Escherichia coli O157
dc.subject.otherIndex gene expression
dc.subject.otherIndex minimum bactericidal concentration
dc.subject.otherIndex minimum inhibitory concentration
dc.subject.otherIndex nonhuman
dc.subject.otherIndex priority journal
dc.subject.otherIndex protein expression
dc.subject.otherIndex protein synthesis
dc.subject.otherIndex RNA transcription
dc.subject.otherIndex Anti-Infective Agents
dc.subject.otherIndex Azithromycin
dc.subject.otherIndex DNA, Bacterial
dc.subject.otherIndex Escherichia coli O157
dc.subject.otherIndex Gene Expression Regulation, Bacterial
dc.subject.otherIndex Genes, Bacterial
dc.subject.otherIndex Gentamicins
dc.subject.otherIndex Humans
dc.subject.otherIndex Imipenem
dc.subject.otherIndex Latex Fixation Tests
dc.subject.otherIndex Microbial Sensitivity Tests
dc.subject.otherIndex Norfloxacin
dc.subject.otherIndex Prophages
dc.subject.otherIndex Real-Time Polymerase Chain Reaction
dc.subject.otherIndex Rifampin
dc.subject.otherIndex Shiga Toxin
dc.subject.otherIndex SOS Response (Genetics)
dc.subject.otherKeywordPlus MEMBRANE-VESICLES
dc.subject.otherKeywordPlus GENE-EXPRESSION
dc.subject.otherKeywordPlus O157-H7
dc.subject.otherKeywordPlus ANTIBIOTICS
dc.subject.otherKeywordPlus INHIBITION
dc.subject.otherKeywordPlus INDUCTION
dc.subject.otherKeywordPlus DNA
dc.subject.otherKeywordPlus TRANSCRIPTION
dc.subject.otherKeywordPlus NORFLOXACIN
dc.subject.otherKeywordPlus RIFAMPICIN
dc.subject.otherWOS Food Science and Technology


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