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No evidence of HTLV-II infection among immonoblot indeterminate samples using nested PCR in Mashhad, Northeast of Iran

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dc.contributor.author Rafatpanah H.
dc.contributor.author Fathimoghadam F.
dc.contributor.author Shahabi M.
dc.contributor.author Eftekharzadeh I.
dc.contributor.author Hedayati-Moghaddam M.
dc.contributor.author Valizadeh N.
dc.contributor.author Tadayon M.
dc.contributor.author Aliakbar Shamsian S.
dc.contributor.author Bidkhori H.
dc.contributor.author Miri R.
dc.contributor.author Bazarbachi A.
dc.contributor.editor
dc.date 2013
dc.date.accessioned 2017-10-05T15:38:19Z
dc.date.available 2017-10-05T15:38:19Z
dc.date.issued 2013
dc.identifier
dc.identifier.isbn
dc.identifier.issn 20083866
dc.identifier.uri http://hdl.handle.net/10938/16270
dc.description.abstract Objective(s): Although HTLV-I infection is endemic in different geographical parts of the world including Northeast of Iran, there have been no documents of HTLV-II infection in this region. It is reported that one possible reason for seroindeterminate state in HTLV western blot is HTLV-II virus. This study aimed to investigate the presence of HTLV-II among blood donors with seroindeterminate western blot results. Materials and Methods: Three ml whole blood obtained from 50 blood donors referring to Mashhad Blood Transfusion Organization who had reactive Elisa for HTLV-I and seroindeterminate HTLV western blot state. A conventional PCR was applied to detect HTLV-I provirus using specific primers while a nested PCR was designed with specific external and internal primers for the detection of HTLV-II. Results: The average age of participants, 39 males and 11 females, was 37.12± 14.36 years. The average OD of the Elisa assay was 1.767± 1.195. The most common indeterminate patterns were Rgp46-II alone (n=12, 27.3percent), Rgp46-I alone (n=7, 15.9percent), and Rgp46-I with GD21 (n=7, 15.9percent).After introducing the DNA to the PCR tests, results revealed 10 (20percent) HTLV-I PCR positive samples while no HTLV-II positive sample was detected by nested PCR. There were no significant age, blood group, Optical Density of the Elisa assay, and western blot indeterminate pattern differences between HTLV-I PCR positive and negative samples. Conclusion: No HTLV-II positive sample was detected in this study which confirms the absence of HTLV-II infection in this region. However, high frequency of HTLV-I PCR positive samples among the seroindeterminate cases implies on the important role of molecular techniques for further confirmation of the infection. © 2013 mums.ac.ir All rights reserved.
dc.format.extent
dc.format.extent Pages: (229-234)
dc.language English
dc.publisher MASHHAD
dc.relation.ispartof Publication Name: Iranian Journal of Basic Medical Sciences; Publication Year: 2013; Volume: 16; no. 3; Pages: (229-234);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other GENBANK: M10060
dc.title No evidence of HTLV-II infection among immonoblot indeterminate samples using nested PCR in Mashhad, Northeast of Iran
dc.type Article
dc.contributor.affiliation Rafatpanah, H., Research Centre for HIV-AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran, Inflammation and Inflammatory Diseases Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
dc.contributor.affiliation Fathimoghadam, F., Research Centre for HIV-AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran
dc.contributor.affiliation Shahabi, M., Research Centre for the Iranian Blood Transfusion Organization (IBTO), Tehran, Iran
dc.contributor.affiliation Eftekharzadeh, I., Research Centre for HIV-AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran
dc.contributor.affiliation Hedayati-Moghaddam, M., Research Centre for HIV-AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran
dc.contributor.affiliation Valizadeh, N., Inflammation and Inflammatory Diseases Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
dc.contributor.affiliation Tadayon, M., Research Centre for HIV-AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran, Islamic Azad University, Mashhad Branch, Iran
dc.contributor.affiliation Aliakbar Shamsian, S., Research Centre for HIV-AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran
dc.contributor.affiliation Bidkhori, H., Research Centre for HIV-AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran
dc.contributor.affiliation Miri, R., Research Centre for HIV-AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran
dc.contributor.affiliation Bazarbachi, A., Department of Internal Medicine, American University of Beirut, Beirut, 10, Lebanon
dc.contributor.authorAddress Fathimoghadam, F.; Research Centre for HIV-AIDS, HTLV and Viral Hepatitis, Iranian Academic Centre for Education, Culture and Research (ACECR), Mashhad Branch, Mashhad, Iran; email: fohrad@gmail.com
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Internal Medicine;
dc.contributor.authorDepartment Internal Medicine
dc.contributor.authorDivision
dc.contributor.authorEmail fohrad@gmail.com
dc.contributor.authorFaculty Faculty of Medicine
dc.contributor.authorInitials Rafatpanah, H
dc.contributor.authorInitials Fathimoghadam, F
dc.contributor.authorInitials Shahabi, M
dc.contributor.authorInitials Eftekharzadeh, I
dc.contributor.authorInitials Hedayati-Moghaddam, M
dc.contributor.authorInitials Valizadeh, N
dc.contributor.authorInitials Tadayon, M
dc.contributor.authorInitials Shamsian, SA
dc.contributor.authorInitials Bidkhori, H
dc.contributor.authorInitials Miri, R
dc.contributor.authorInitials Bazarbachi, A
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Fathimoghadam, F (reprint author), Iranian Acad Ctr Educ Culture and Res ACECR, Res Ctr HIV AIDS HTLV and Viral Hepatitis, Mashhad Branch, Mashhad, Iran.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut Medical Center
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dc.description.citedTotWOSCount 0
dc.description.citedWOSCount 0
dc.format.extentCount 6
dc.identifier.articleNo
dc.identifier.coden
dc.identifier.pubmedID
dc.identifier.scopusID 84877706524
dc.identifier.url
dc.publisher.address VICE-CHANCELLOR FOR RES CTR OFF IJBMS, DANESHGAH ST, PO BOX 9138813944 - 445, MASHHAD, 00000, IRAN
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dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr Iran. J. Basic Med. Sci.
dc.relation.ispartOfIssue 3
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Iranian Journal of Basic Medical Sciences
dc.relation.ispartofPubTitleAbbr Iran. J. Basic Med. Sci.
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 16
dc.source.ID WOS:000330319500010
dc.type.publication Journal
dc.subject.otherAuthKeyword HTLV-I
dc.subject.otherAuthKeyword HTLV-II
dc.subject.otherAuthKeyword Iran mashhad
dc.subject.otherAuthKeyword PCR
dc.subject.otherAuthKeyword Seroindeterminate
dc.subject.otherAuthKeyword Western blot
dc.subject.otherChemCAS
dc.subject.otherIndex adult
dc.subject.otherIndex article
dc.subject.otherIndex blood donor
dc.subject.otherIndex clinical article
dc.subject.otherIndex controlled study
dc.subject.otherIndex cross-sectional study
dc.subject.otherIndex DNA extraction
dc.subject.otherIndex enzyme linked immunosorbent assay
dc.subject.otherIndex female
dc.subject.otherIndex human
dc.subject.otherIndex Human T cell leukemia virus 1
dc.subject.otherIndex Human T cell leukemia virus 2
dc.subject.otherIndex Human T cell leukemia virus infection
dc.subject.otherIndex immunoblotting
dc.subject.otherIndex male
dc.subject.otherIndex nonhuman
dc.subject.otherIndex nucleotide sequence
dc.subject.otherIndex polymerase chain reaction
dc.subject.otherIndex Western blotting
dc.subject.otherKeywordPlus VIRUS TYPE-I
dc.subject.otherKeywordPlus WESTERN-BLOT PATTERNS
dc.subject.otherKeywordPlus POLYMERASE-CHAIN-REACTION
dc.subject.otherKeywordPlus NEUROLOGICAL DISEASE
dc.subject.otherKeywordPlus MOLECULAR EVIDENCE
dc.subject.otherKeywordPlus ASSAY
dc.subject.otherKeywordPlus EPIDEMIOLOGY
dc.subject.otherKeywordPlus INDIVIDUALS
dc.subject.otherKeywordPlus DONORS
dc.subject.otherKeywordPlus DIFFERENTIATION
dc.subject.otherWOS Medicine, Research and Experimental
dc.subject.otherWOS Pharmacology and Pharmacy


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