AUB ScholarWorks

Ubiquitylated Tax targets and binds the IKK signalosome at the centrosome

Show simple item record

dc.contributor.author Kfoury Y.
dc.contributor.author Nasr R.
dc.contributor.author Favre-Bonvin A.
dc.contributor.author El-Sabban M.
dc.contributor.author Renault N.
dc.contributor.author Giron M.-L.
dc.contributor.author Setterblad N.
dc.contributor.author Hajj H.E.
dc.contributor.author Chiari E.
dc.contributor.author Mikati A.G.
dc.contributor.author Hermine O.
dc.contributor.author Saib A.
dc.contributor.author De The H.
dc.contributor.author Pique C.
dc.contributor.author Bazarbachi A.
dc.contributor.editor
dc.date Mar-2008
dc.date.accessioned 2017-10-05T15:39:10Z
dc.date.available 2017-10-05T15:39:10Z
dc.date.issued 2008
dc.identifier 10.1038/sj.onc.1210804
dc.identifier.isbn
dc.identifier.issn 09509232
dc.identifier.uri http://hdl.handle.net/10938/16701
dc.description.abstract Constitutive activation of the NF-κB pathway by the Tax oncoprotein plays a crucial role in the proliferation and transformation of HTLV-I infected T lymphocytes. We have previously shown that Tax ubiquitylation on C-terminal lysines is critical for binding of Tax to IκB kinase (IKK) and its subsequent activation. Here, we report that ubiquitylated Tax is not associated with active cytosolic IKK subunits, but binds endogenous IKK-α, -β, -γ, targeting them to the centrosome. K63-ubiquitylated Tax colocalizes at the centrosome with IKK-γ, while K48-ubiquitylated Tax is stabilized upon proteasome inhibition. Altogether, these results support a model in which K63-ubiquitylated Tax activates IKK in a centrosome-associated signalosome, leading to the production of Tax-free active cytoplasmic IKK. These observations highlight an unsuspected link between Tax-induced IKK activation and the centrosome. © 2008 Nature Publishing Group All rights reserved.
dc.format.extent
dc.format.extent Pages: (1665-1676)
dc.language English
dc.publisher LONDON
dc.relation.ispartof Publication Name: Oncogene; Publication Year: 2008; Volume: 27; no. 12; Pages: (1665-1676);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other
dc.title Ubiquitylated Tax targets and binds the IKK signalosome at the centrosome
dc.type Article
dc.contributor.affiliation Kfoury, Y., Department of Internal Medicine, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Nasr, R., Department of Internal Medicine, American University of Beirut, Beirut, Lebanon, UMR 7151 CNRS, Laboratoire Associé Au Comité de Paris de la Ligue Contre Le Cancer, Paris, France
dc.contributor.affiliation Favre-Bonvin, A., UMR 7151 CNRS-Université Paris 7, INSERM U567, Département de Biologie Cellulaire, Paris, France
dc.contributor.affiliation El-Sabban, M., Department of Human Morphology, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Renault, N., UMR 7151 CNRS, Laboratoire Associé Au Comité de Paris de la Ligue Contre Le Cancer, Paris, France
dc.contributor.affiliation Giron, M.-L., UMR 7151 CNRS, Laboratoire Associé Au Comité de Paris de la Ligue Contre Le Cancer, Paris, France
dc.contributor.affiliation Setterblad, N., Imagery Department, Institut Universitaire d'Hématologie IFR105 Paris, France
dc.contributor.affiliation Hajj, H.E., Department of Internal Medicine, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Chiari, E., UMR 7151 CNRS-Université Paris 7, INSERM U567, Département de Biologie Cellulaire, Paris, France
dc.contributor.affiliation Mikati, A.G., Department of Internal Medicine, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Hermine, O., CNRS UMR 8603, Department of Hematology, Necker Hospital, Paris, France
dc.contributor.affiliation Saib, A., UMR 7151 CNRS, Laboratoire Associé Au Comité de Paris de la Ligue Contre Le Cancer, Paris, France
dc.contributor.affiliation De Thé, H., UMR 7151 CNRS, Laboratoire Associé Au Comité de Paris de la Ligue Contre Le Cancer, Paris, France
dc.contributor.affiliation Pique, C., UMR 7151 CNRS-Université Paris 7, INSERM U567, Département de Biologie Cellulaire, Paris, France
dc.contributor.affiliation Bazarbachi, A., Department of Internal Medicine, American University of Beirut, Beirut, Lebanon, Department of Internal Medicine, American University of Beirut, Medical Center, Hamra Street, Beirut, Lebanon
dc.contributor.authorAddress Bazarbachi, A.; Department of Internal Medicine, American University of Beirut, Medical Center, Hamra Street, Beirut, Lebanon; email: bazarbac@aub.edu.lb
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Internal Medicine;
dc.contributor.authorDepartment Internal Medicine
dc.contributor.authorDivision
dc.contributor.authorEmail bazarbac@aub.edu.lb
dc.contributor.authorFaculty Faculty of Medicine
dc.contributor.authorInitials Kfoury, Y
dc.contributor.authorInitials Nasr, R
dc.contributor.authorInitials Favre-Bonvin, A
dc.contributor.authorInitials El-Sabban, M
dc.contributor.authorInitials Renault, N
dc.contributor.authorInitials Giron, ML
dc.contributor.authorInitials Setterblad, N
dc.contributor.authorInitials El Hajj, H
dc.contributor.authorInitials Chiari, E
dc.contributor.authorInitials Mikati, AG
dc.contributor.authorInitials Hermine, O
dc.contributor.authorInitials Saib, A
dc.contributor.authorInitials de The, H
dc.contributor.authorInitials Pique, C
dc.contributor.authorInitials Bazarbachi, A
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Bazarbachi, A (reprint author), Amer Univ Beirut, Med Ctr, Dept Internal Med, Hamra St,POB 1136044, Beirut, Lebanon.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut Medical Center
dc.description.cited Bex F, 1998, MOL CELL BIOL, V18, P2392; Cheng TS, 2006, LIFE SCI, V78, P1114, DOI 10.1016-j.lfs.2005.06.021; Chiari E, 2004, J VIROL, V78, P11823, DOI 10.1128-JVI.78.21.11823-11832.2004; Ching YP, 2006, NAT CELL BIOL, V8, P717, DOI 10.1038-ncb1432; Conaway RC, 2002, SCIENCE, V296, P1254, DOI 10.1126-science.1067466; Deng L, 2000, CELL, V103, P351, DOI 10.1016-S0092-8674(00)00126-4; Ea CK, 2006, MOL CELL, V22, P245, DOI 10.1016-j.molcel.2006.03.026; Harhaj NS, 2007, J BIOL CHEM, V282, P4185, DOI 10.1074-jbc.M611031200; Huang TT, 2003, CELL, V115, P565, DOI 10.1016-S0092-8674(03)00895-X; Kovalenko A, 2003, NATURE, V424, P801, DOI 10.1038-nature01802; Lamsoul I, 2005, MOL CELL BIOL, V25, P10391, DOI 10.1128-MCB.25.23.10391-10406.2005; Nasr R, 2006, BLOOD, V107, P4021, DOI 10.1182-blood-2005-09-3572; Nawrocki ST, 2006, CANCER RES, V66, P3773, DOI 10.1158-0008-5472.CAN-05-2961; Nejmeddine M, 2005, J BIOL CHEM, V280, P29653, DOI 10.1074-jbc.M502639200; Nitta T, 2006, CANCER SCI, V97, P836, DOI 10.1111-j.1349-7006.2006.00254.x; Peloponese JM, 2004, J VIROL, V78, P11686, DOI 10.1128-JVI.78.21.11686-11695.2004; Pumfery A, 2006, RETROVIROLOGY, V3, DOI 10.1186-1742-4690-3-50; Robek MD, 1999, J VIROL, V73, P4856; Sun SC, 2000, AIDS RES HUM RETROV, V16, P1591, DOI 10.1089-08892220050193001; Wu CJ, 2006, NAT CELL BIOL, V8, P398, DOI 10.1038-ncb1384
dc.description.citedCount 34
dc.description.citedTotWOSCount 34
dc.description.citedWOSCount 34
dc.format.extentCount 12
dc.identifier.articleNo
dc.identifier.coden ONCNE
dc.identifier.pubmedID 17891179
dc.identifier.scopusID 40749092487
dc.identifier.url
dc.publisher.address MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
dc.relation.ispartofConference
dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr Oncogene
dc.relation.ispartOfIssue 12
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Oncogene
dc.relation.ispartofPubTitleAbbr Oncogene
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 27
dc.source.ID WOS:000253962100002
dc.type.publication Journal
dc.subject.otherAuthKeyword Centrosome
dc.subject.otherAuthKeyword HTLV-I
dc.subject.otherAuthKeyword IkκB kinase
dc.subject.otherAuthKeyword NF-κB
dc.subject.otherAuthKeyword Tax
dc.subject.otherAuthKeyword Ubiquitin
dc.subject.otherChemCAS proteasome, 140879-24-9
dc.subject.otherChemCAS Carrier Proteins
dc.subject.otherChemCAS Gene Products, tax
dc.subject.otherChemCAS I-kappa B Kinase, EC 2.7.1.37
dc.subject.otherChemCAS IKBKAP protein, human
dc.subject.otherChemCAS IKBKB protein, human, EC 2.7.11.10
dc.subject.otherChemCAS IKBKG protein, human
dc.subject.otherChemCAS Protein Subunits
dc.subject.otherChemCAS Ubiquitin
dc.subject.otherIndex cytoplasm protein
dc.subject.otherIndex I kappa B kinase alpha
dc.subject.otherIndex I kappa B kinase beta
dc.subject.otherIndex I kappa B kinase gamma
dc.subject.otherIndex lysine derivative
dc.subject.otherIndex proteasome
dc.subject.otherIndex protein subunit
dc.subject.otherIndex Tax protein
dc.subject.otherIndex tumor necrosis factor alpha
dc.subject.otherIndex article
dc.subject.otherIndex centrosome
dc.subject.otherIndex controlled study
dc.subject.otherIndex cytoplasm
dc.subject.otherIndex human
dc.subject.otherIndex human cell
dc.subject.otherIndex priority journal
dc.subject.otherIndex protein binding
dc.subject.otherIndex protein expression
dc.subject.otherIndex protein induction
dc.subject.otherIndex protein localization
dc.subject.otherIndex protein protein interaction
dc.subject.otherIndex protein stability
dc.subject.otherIndex protein targeting
dc.subject.otherIndex signal transduction
dc.subject.otherIndex ubiquitination
dc.subject.otherIndex Carrier Proteins
dc.subject.otherIndex Cell Line, Transformed
dc.subject.otherIndex Centrosome
dc.subject.otherIndex Enzyme Activation
dc.subject.otherIndex Gene Products, tax
dc.subject.otherIndex Hela Cells
dc.subject.otherIndex Human T-lymphotropic virus 1
dc.subject.otherIndex Humans
dc.subject.otherIndex I-kappa B Kinase
dc.subject.otherIndex Protein Binding
dc.subject.otherIndex Protein Subunits
dc.subject.otherIndex Signal Transduction
dc.subject.otherIndex Ubiquitin
dc.subject.otherIndex Human T-lymphotropic virus 1
dc.subject.otherKeywordPlus T-CELL LEUKEMIA
dc.subject.otherKeywordPlus NF-KAPPA-B
dc.subject.otherKeywordPlus VIRUS TYPE-1 TAX
dc.subject.otherKeywordPlus I TAX
dc.subject.otherKeywordPlus ACTIVATION
dc.subject.otherKeywordPlus PROTEIN
dc.subject.otherKeywordPlus UBIQUITINATION
dc.subject.otherKeywordPlus ONCOPROTEIN
dc.subject.otherKeywordPlus KINASE
dc.subject.otherKeywordPlus AMPLIFICATION
dc.subject.otherWOS Biochemistry and Molecular Biology
dc.subject.otherWOS Oncology
dc.subject.otherWOS Cell Biology
dc.subject.otherWOS Genetics and Heredity


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search AUB ScholarWorks


Browse

My Account