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Tax ubiquitylation and SUMOylation control the dynamic shuttling of Tax and NEMO between Ubc9 nuclear bodies and the centrosome

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dc.contributor.author Kfoury Y.
dc.contributor.author Setterblad N.
dc.contributor.author El-Sabban M.
dc.contributor.author Zamborlini A.
dc.contributor.author Dassouki Z.
dc.contributor.author El Hajj H.
dc.contributor.author Hermine O.
dc.contributor.author Pique C.
dc.contributor.author De The H.
dc.contributor.author Saib A.
dc.contributor.author Bazarbachi A.
dc.contributor.editor
dc.date Jan-2011
dc.date.accessioned 2017-10-05T15:39:37Z
dc.date.available 2017-10-05T15:39:37Z
dc.date.issued 2011
dc.identifier 10.1182/blood-2010-05-285742
dc.identifier.isbn
dc.identifier.issn 00064971
dc.identifier.uri http://hdl.handle.net/10938/16992
dc.description.abstract The human T-lymphotropic virus type I oncoprotein Tax is critical for T-cell transformation, acting mainly through nuclear factor kappa B essential modulator (NEMO) binding and subsequent nuclear factor-κB activation. Tax localizes to Tax nuclear bodies and to the centrosome and is subjected to ubiquitylation and small ubiquitin-like modifier (SUMO)ylation, which are both necessary for complete transcriptional activation. Using the photoconvertible fluorophore Dendra-2 coupled with live video confocal microscopy, we show for the first time that the same Tax molecule shuttles among Tax nuclear bodies and between these nuclear bodies and the centrosome, depending on its posttranslational modifications. Ubiquitylation targets Tax to nuclear bodies to which NEMO is recruited and subsequently SUMOylated. We also demonstrate that Tax nuclear bodies contain the SUMOylation machinery including SUMO and the SUMO conjugating enzyme Ubc9, strongly suggesting that these nuclear bodies represent sites of active SUMOylation. Finally, both ubiquitylation and SUMOylation of Tax control NEMO targeting to the centrosome. Altogether, we are proposing a model where both ubiquitylation and SUMOylation of Tax control the shuttling of Tax and NEMO between the cytoplasmic and nuclear compartments. © 2011 by The American Society of Hematology.
dc.format.extent
dc.format.extent Pages: (190-199)
dc.language English
dc.publisher WASHINGTON
dc.relation.ispartof Publication Name: Blood; Publication Year: 2011; Volume: 117; no. 1; Pages: (190-199);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other
dc.title Tax ubiquitylation and SUMOylation control the dynamic shuttling of Tax and NEMO between Ubc9 nuclear bodies and the centrosome
dc.type Article
dc.contributor.affiliation Kfoury, Y., Department of Internal Medicine, American University of Beirut, PO Box 113-6044, Beirut, Lebanon
dc.contributor.affiliation Setterblad, N., Imagery Department, Institut Universitaire d'Hématologie, Institut Fédératif de Recherche 105, Paris, France
dc.contributor.affiliation El-Sabban, M., Department of Human Morphology, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Zamborlini, A., Unité Mixte de Recherche (UMR) 7212 Centre National de la Recherche Scientifique (CNRS), U944 Inserm, Laboratoire Associé au Comité de Paris de la Ligue Contre le Cancer, Paris, France
dc.contributor.affiliation Dassouki, Z., Department of Internal Medicine, American University of Beirut, PO Box 113-6044, Beirut, Lebanon
dc.contributor.affiliation El Hajj, H., Department of Internal Medicine, American University of Beirut, PO Box 113-6044, Beirut, Lebanon
dc.contributor.affiliation Hermine, O., CNRS UMR 8603, Department of Hematology, Necker Hospital, Paris, France
dc.contributor.affiliation Pique, C., Université Paris Descartes, Institut Cochin, Department of Cell Biology and Host-Pathogens Interactions, Paris, France
dc.contributor.affiliation De Thé, H., Unité Mixte de Recherche (UMR) 7212 Centre National de la Recherche Scientifique (CNRS), U944 Inserm, Laboratoire Associé au Comité de Paris de la Ligue Contre le Cancer, Paris, France
dc.contributor.affiliation Saïb, A., Unité Mixte de Recherche (UMR) 7212 Centre National de la Recherche Scientifique (CNRS), U944 Inserm, Laboratoire Associé au Comité de Paris de la Ligue Contre le Cancer, Paris, France, Conservatoire National des Arts et Métiers, Chaire de Biologie, Paris, France
dc.contributor.affiliation Bazarbachi, A., Department of Internal Medicine, American University of Beirut, PO Box 113-6044, Beirut, Lebanon
dc.contributor.authorAddress Saïb, A.; CNRS UMR 7212, Institut Universitaire d'Hématologie, Hôpital Saint-Louis, Paris, France; email: ali.saib@univ-paris-diderot.fr
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Internal Medicine;
dc.contributor.authorDepartment Internal Medicine
dc.contributor.authorDivision
dc.contributor.authorEmail ali.saib@univ-paris-diderot.fr; bazarbac@aub.edu.lb
dc.contributor.faculty Faculty of Medicine
dc.contributor.authorInitials Kfoury, Y
dc.contributor.authorInitials Setterblad, N
dc.contributor.authorInitials El-Sabban, M
dc.contributor.authorInitials Zamborlini, A
dc.contributor.authorInitials Dassouki, Z
dc.contributor.authorInitials El Hajj, H
dc.contributor.authorInitials Hermine, O
dc.contributor.authorInitials Pique, C
dc.contributor.authorInitials De The, H
dc.contributor.authorInitials Saib, A
dc.contributor.authorInitials Bazarbachi, A
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Bazarbachi, A (reprint author), Amer Univ Beirut, Dept Internal Med, POB 113-6044, Beirut, Lebanon.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut Medical Center
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dc.description.citedCount 25
dc.description.citedTotWOSCount 26
dc.description.citedWOSCount 25
dc.format.extentCount 10
dc.identifier.articleNo
dc.identifier.coden BLOOA
dc.identifier.pubmedID 20959607
dc.identifier.scopusID 78650985961
dc.identifier.url
dc.publisher.address 1900 M STREET. NW SUITE 200, WASHINGTON, DC 20036 USA
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dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr Blood
dc.relation.ispartOfIssue 1
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Blood
dc.relation.ispartofPubTitleAbbr Blood
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 117
dc.source.ID WOS:000285963900029
dc.type.publication Journal
dc.subject.otherAuthKeyword
dc.subject.otherChemCAS ubiquitin conjugating enzyme, 147154-16-3
dc.subject.otherChemCAS Gene Products, tax
dc.subject.otherChemCAS I-kappa B Kinase, 2.7.11.10
dc.subject.otherChemCAS IKBKG protein, human
dc.subject.otherChemCAS NF-kappa B
dc.subject.otherChemCAS RNA, Messenger
dc.subject.otherChemCAS Ubiquitin
dc.subject.otherChemCAS Ubiquitin-Conjugating Enzymes, 6.3.2.19
dc.subject.otherChemCAS ubiquitin-conjugating enzyme UBC9, 6.3.2.19
dc.subject.otherIndex I kappa B kinase gamma
dc.subject.otherIndex SUMO protein
dc.subject.otherIndex Tax protein
dc.subject.otherIndex ubiquitin conjugating enzyme
dc.subject.otherIndex ubiquitin conjugating enzyme 9
dc.subject.otherIndex unclassified drug
dc.subject.otherIndex animal cell
dc.subject.otherIndex article
dc.subject.otherIndex cell nucleus inclusion body
dc.subject.otherIndex centrosome
dc.subject.otherIndex human
dc.subject.otherIndex human cell
dc.subject.otherIndex mouse
dc.subject.otherIndex nonhuman
dc.subject.otherIndex priority journal
dc.subject.otherIndex sumoylation
dc.subject.otherIndex ubiquitination
dc.subject.otherIndex Blotting, Western
dc.subject.otherIndex Cell Nucleus
dc.subject.otherIndex Cells, Cultured
dc.subject.otherIndex Centrosome
dc.subject.otherIndex Cytoplasm
dc.subject.otherIndex Embryo, Mammalian
dc.subject.otherIndex Fibroblasts
dc.subject.otherIndex Fluorescent Antibody Technique
dc.subject.otherIndex Gene Products, tax
dc.subject.otherIndex Humans
dc.subject.otherIndex I-kappa B Kinase
dc.subject.otherIndex Kidney
dc.subject.otherIndex Lung
dc.subject.otherIndex NF-kappa B
dc.subject.otherIndex Protein Binding
dc.subject.otherIndex Protein Processing, Post-Translational
dc.subject.otherIndex Reverse Transcriptase Polymerase Chain Reaction
dc.subject.otherIndex RNA, Messenger
dc.subject.otherIndex Signal Transduction
dc.subject.otherIndex Sumoylation
dc.subject.otherIndex Transcriptional Activation
dc.subject.otherIndex Ubiquitin
dc.subject.otherIndex Ubiquitin-Conjugating Enzymes
dc.subject.otherIndex Ubiquitination
dc.subject.otherKeywordPlus LEUKEMIA-VIRUS TYPE-1
dc.subject.otherKeywordPlus KAPPA-B ACTIVATION
dc.subject.otherKeywordPlus VIROLOGICAL-SYNAPSE
dc.subject.otherKeywordPlus CONJUGATING ENZYME
dc.subject.otherKeywordPlus GENOTOXIC STRESS
dc.subject.otherKeywordPlus KINASE COMPLEX
dc.subject.otherKeywordPlus IKK-GAMMA
dc.subject.otherKeywordPlus PROTEIN
dc.subject.otherKeywordPlus UBIQUITINATION
dc.subject.otherKeywordPlus ONCOPROTEIN
dc.subject.otherWOS Hematology


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