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Phylogenetic assessment of heterotrophic bacteria from a water distribution system using 16S rDNA sequencing

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dc.contributor.author Tokajian S.T.
dc.contributor.author Hashwa F.A.
dc.contributor.author Hancock I.C.
dc.contributor.author Zalloua P.A.
dc.contributor.editor
dc.date 2005
dc.date.accessioned 2017-10-05T15:40:11Z
dc.date.available 2017-10-05T15:40:11Z
dc.date.issued 2005
dc.identifier 10.1139/w05-007
dc.identifier.isbn
dc.identifier.issn 00084166
dc.identifier.uri http://hdl.handle.net/10938/17295
dc.description.abstract Determination of a heterotrophic plate count (HPC) for drinking-water samples alone is not enough to assess possible health hazards associated with sudden changes in the bacterial count. Speciation is very crucial to determine whether the population includes pathogens and (or) opportunistic pathogens. Most of the isolates recovered from drinking water samples could not be allocated to a specific phylogenetic branch based on the use of conventional diagnostic methods. The present study had to use phylogenetic analysis, which was simplified by determining and using the first 500-bp sequence of the 16S rDNA, to successfully identify the type and species of bacteria found in the samples. Gram-positive bacteria α-, β-, and γ-Proteobacteria were found to be the major groups representing the heterotrophic bacteria in drinking water. The study also revealed that the presence of sphingomonads in drinking water supplies may be much more common than has been reported so far and thus further studies are merited. The intermittent mode of supply, mainly characterized by water stagnation and flow interruption associated possibly with biofilm detachment, raised the possibility that the studied bacterial populations in such systems represented organisms coming from 2 different niches, the biofilm and the water column. © 2005 NRC Canada.
dc.format.extent
dc.format.extent Pages: (325-335)
dc.language English
dc.publisher OTTAWA
dc.relation.ispartof Publication Name: Canadian Journal of Microbiology; Publication Year: 2005; Volume: 51; no. 4; Pages: (325-335);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other GENBANK: AB025279, AB025572, AB027623, AB033950, AF004949, AF005021, AF101418, AF137029, AF137381, AF191343, AF197055, AF397061, AF503277, AF509480, AF510045, AF514798, AF531770, AF544629, AF547958, AJ001011, AJ227775, AJ227796, AJ250434, AJ250800, AJ2762
dc.title Phylogenetic assessment of heterotrophic bacteria from a water distribution system using 16S rDNA sequencing
dc.type Article
dc.contributor.affiliation Tokajian, S.T., Department of Biology, Lebanese American University, P.O. Box 36, Byblos, Lebanon
dc.contributor.affiliation Hashwa, F.A., Department of Biology, Lebanese American University, P.O. Box 36, Byblos, Lebanon
dc.contributor.affiliation Hancock, I.C., School of Cell and Molecular Biosciences, Medical School, University of Newcastle Upon-tyne, Framlington Place, Newcastle upon Tyne, NE2 4HH, United Kingdom
dc.contributor.affiliation Zalloua, P.A., American University of Beirut, Riad El Solh, Beirut 1107 2020, Lebanon
dc.contributor.authorAddress Hashwa, F.A.; Department of Biology, Lebanese American University, P.O. Box 36, Byblos, Lebanon; email: fhashwa@lau.edu.lb
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Internal Medicine;
dc.contributor.authorDepartment Internal Medicine
dc.contributor.authorDivision
dc.contributor.authorEmail fhashwa@lau.edu.lb
dc.contributor.authorFaculty Faculty of Medicine
dc.contributor.authorInitials Tokajian, ST
dc.contributor.authorInitials Hashwa, FA
dc.contributor.authorInitials Hancock, IC
dc.contributor.authorInitials Zalloua, PA
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Hashwa, FA (reprint author), Lebanese Amer Univ, Dept Biol, POB 36, Byblos, Lebanon.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut Medical Center
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dc.description.citedCount 30
dc.description.citedTotWOSCount 30
dc.description.citedWOSCount 28
dc.format.extentCount 11
dc.identifier.articleNo
dc.identifier.coden CJMIA
dc.identifier.pubmedID 15980895
dc.identifier.scopusID 23844488101
dc.identifier.url
dc.publisher.address RESEARCH JOURNALS, MONTREAL RD, OTTAWA, ONTARIO K1A 0R6, CANADA
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dc.relation.ispartofConferenceDate
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dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr Can. J. Microbiol.
dc.relation.ispartOfIssue 4
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Canadian Journal of Microbiology
dc.relation.ispartofPubTitleAbbr Can. J. Microbiol.
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 51
dc.source.ID WOS:000230893600007
dc.type.publication Journal
dc.subject.otherAuthKeyword 16S rDNA
dc.subject.otherAuthKeyword Biofilm
dc.subject.otherAuthKeyword Intermittent water supply
dc.subject.otherAuthKeyword Water column
dc.subject.otherChemCAS DNA, Bacterial
dc.subject.otherChemCAS DNA, Ribosomal
dc.subject.otherChemCAS RNA, Ribosomal, 16S
dc.subject.otherIndex Biofilms
dc.subject.otherIndex DNA
dc.subject.otherIndex Flow of fluids
dc.subject.otherIndex Genetic engineering
dc.subject.otherIndex Health hazards
dc.subject.otherIndex Potable water
dc.subject.otherIndex Water distribution systems
dc.subject.otherIndex Water supply
dc.subject.otherIndex 16S rDNA
dc.subject.otherIndex Heterotrophic plate count (HPC)
dc.subject.otherIndex Intermittent water supply
dc.subject.otherIndex Water column
dc.subject.otherIndex Bacteria
dc.subject.otherIndex drinking water
dc.subject.otherIndex ribosome DNA
dc.subject.otherIndex bacterium
dc.subject.otherIndex community composition
dc.subject.otherIndex drinking water
dc.subject.otherIndex phylogenetics
dc.subject.otherIndex Alphaproteobacteria
dc.subject.otherIndex article
dc.subject.otherIndex bacterium identification
dc.subject.otherIndex Betaproteobacteria
dc.subject.otherIndex biofilm
dc.subject.otherIndex DNA sequence
dc.subject.otherIndex Gammaproteobacteria
dc.subject.otherIndex Gram positive bacterium
dc.subject.otherIndex heterotrophy
dc.subject.otherIndex nonhuman
dc.subject.otherIndex nucleotide sequence
dc.subject.otherIndex phylogeny
dc.subject.otherIndex priority journal
dc.subject.otherIndex Sphingomonadaceae
dc.subject.otherIndex unindexed sequence
dc.subject.otherIndex water analysis
dc.subject.otherIndex water supply
dc.subject.otherIndex Algorithms
dc.subject.otherIndex DNA, Bacterial
dc.subject.otherIndex DNA, Ribosomal
dc.subject.otherIndex Lebanon
dc.subject.otherIndex Phylogeny
dc.subject.otherIndex Polymerase Chain Reaction
dc.subject.otherIndex Proteobacteria
dc.subject.otherIndex RNA, Ribosomal, 16S
dc.subject.otherIndex Sequence Homology, Nucleic Acid
dc.subject.otherIndex Species Specificity
dc.subject.otherIndex Water Microbiology
dc.subject.otherIndex Water Supply
dc.subject.otherIndex Gammaproteobacteria
dc.subject.otherIndex Posibacteria
dc.subject.otherKeywordPlus RIBOSOMAL-RNA SEQUENCES
dc.subject.otherKeywordPlus DRINKING-WATER
dc.subject.otherKeywordPlus POTABLE WATER
dc.subject.otherKeywordPlus NONTUBERCULOUS MYCOBACTERIA
dc.subject.otherKeywordPlus PSEUDOMONAS-MESOPHILICA
dc.subject.otherKeywordPlus RAPID-DETERMINATION
dc.subject.otherKeywordPlus IDENTIFICATION
dc.subject.otherKeywordPlus AEROMONAS
dc.subject.otherKeywordPlus DIVERSITY
dc.subject.otherKeywordPlus METHYLOBACTERIUM
dc.subject.otherWOS Biochemistry and Molecular Biology
dc.subject.otherWOS Biotechnology and Applied Microbiology
dc.subject.otherWOS Immunology
dc.subject.otherWOS Microbiology


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