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25-Hydroxyvitamin D assay variations and impact on clinical decision making

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dc.contributor.author Barake M.
dc.contributor.author Daher R.T.
dc.contributor.author Salti I.
dc.contributor.author Cortas N.K.
dc.contributor.author Al-Shaar L.
dc.contributor.author Habib R.H.
dc.contributor.author Fuleihan G.E.-H.
dc.contributor.editor
dc.date Mar-2012
dc.date.accessioned 2017-10-05T15:59:33Z
dc.date.available 2017-10-05T15:59:33Z
dc.date.issued 2012
dc.identifier 10.1210/jc.2011-2584
dc.identifier.isbn
dc.identifier.issn
dc.identifier.uri http://hdl.handle.net/10938/19074
dc.description.abstract Context: Laboratories are increasingly shifting to new automated 25-hydroxyvitamin D (25-OHD) assays, with subsequent variability in results. Objective-Setting: We describe the experience at our center with such a shift and illustrate its clinical implications. Methods: 25-OHD levels were measured in 494 patients using Immunodiagnostic Systems RIA (IDS-RIA) and DiaSorin Liaison assays. Sources of variability between the assays were investigated in a subset of 83 samples, retested in the reference laboratory in the United States,andby reviewing the performance reports issued by the International Vitamin D External Quality Assessment Scheme, DEQAS. 25-OHD cut-points for target levels were used to compare the two assays. Results: 25-OHD concentrations were significantly lower when measured with Liaison as compared to IDS-RIA: mean bias was -5 ng-ml, range was -38.1 to 18.7 ng-ml, P 0.001; the absolute bias was independent of 25-OHD value. Interassay variability was also detected in values obtained in the reference laboratory and in DEQAS reports. Using 20 ng-ml as the target 25-OHD level, 52percent of patients required treatment when tested by Liaison, as opposed to 36percent by IDS-RIA (P0.001). Using 30 ng-ml as the desirable level, the proportions were 79 and 64percent, respectively (P0.001). The two assays agreed in only 41-68percent of subjects, proportions that depended on criteria used to define agreement. Conclusion: A change in 25-OHD assays has a significant impact on results, patient classification, and treatment recommendations. Such variability cannot be ignored when deriving and applying vitamin Dguidelines. It also renders universal assay standardization a pressing call. Copyright © 2012 by The Endocrine Society.
dc.format.extent
dc.format.extent Pages: (835-843)
dc.language English
dc.publisher CHEVY CHASE
dc.relation.ispartof Publication Name: Journal of Clinical Endocrinology and Metabolism; Publication Year: 2012; Volume: 97; no. 3; Pages: (835-843);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other
dc.title 25-Hydroxyvitamin D assay variations and impact on clinical decision making
dc.type Article
dc.contributor.affiliation Barake, M., Division of Endocrinology, Department of Internal Medicine, American University of Beirut-Medical Center, P.O. Box 11-0236, Riad El Solh, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Daher, R.T., Department of Pathology and Laboratory Medicine, American University of Beirut-Medical Center, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Salti, I., Division of Endocrinology, Department of Internal Medicine, American University of Beirut-Medical Center, P.O. Box 11-0236, Riad El Solh, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Cortas, N.K., Division of Endocrinology, Department of Internal Medicine, American University of Beirut-Medical Center, P.O. Box 11-0236, Riad El Solh, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Al-Shaar, L., Outcomes Research Unit, American University of Beirut-Medical Center, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Habib, R.H., Outcomes Research Unit, American University of Beirut-Medical Center, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Fuleihan, G.E.-H., Division of Endocrinology, Department of Internal Medicine, American University of Beirut-Medical Center, P.O. Box 11-0236, Riad El Solh, Beirut 1107 2020, Lebanon, Calcium Metabolism and Osteoporosis Program, American University of Beirut-Medical Center, Beirut 1107 2020, Lebanon
dc.contributor.authorAddress Fuleihan, G.E.-H.; Division of Endocrinology, Department of Internal Medicine, American University of Beirut-Medical Center, P.O. Box 11-0236, Riad El Solh, Beirut 1107 2020, Lebanon; email: gf01@aub.edu.lb
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Pathology and Laboratory Medicine;
dc.contributor.authorDepartment Pathology and Laboratory Medicine
dc.contributor.authorDivision
dc.contributor.authorEmail gf01@aub.edu.lb
dc.contributor.authorFaculty Faculty of Medicine
dc.contributor.authorInitials Barake, M
dc.contributor.authorInitials Daher, RT
dc.contributor.authorInitials Salti, I
dc.contributor.authorInitials Cortas, NK
dc.contributor.authorInitials Al-Shaar, L
dc.contributor.authorInitials Habib, RH
dc.contributor.authorInitials El-Hajj Fuleihan, G
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress El-Hajj Fuleihan, G (reprint author), Amer Univ Beirut, Med Ctr,Div Endocrinol, Dept Internal Med,Calcium Metab and Osteoporosis Pr, World Hlth Org Collaborating Ctr Metab Bone Disor, POB 11-0236, Beirut 11072020, Lebanon.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut Medical Center
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dc.description.citedCount 24
dc.description.citedTotWOSCount 22
dc.description.citedWOSCount 22
dc.format.extentCount 9
dc.identifier.articleNo
dc.identifier.coden JCEMA
dc.identifier.pubmedID 22238386
dc.identifier.scopusID 84858009750
dc.identifier.url
dc.publisher.address 8401 CONNECTICUT AVE, SUITE 900, CHEVY CHASE, MD 20815-5817 USA
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dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr J. Clin. Endocrinol. Metab.
dc.relation.ispartOfIssue 3
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Journal of Clinical Endocrinology and Metabolism
dc.relation.ispartofPubTitleAbbr J. Clin. Endocrinol. Metab.
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 97
dc.source.ID WOS:000301229600048
dc.type.publication Journal
dc.subject.otherAuthKeyword
dc.subject.otherChemCAS 25 hydroxyvitamin D, 64719-49-9
dc.subject.otherChemCAS 25-hydroxyvitamin D, 64719-49-9
dc.subject.otherChemCAS Vitamin D, 1406-16-2
dc.subject.otherIndex 25 hydroxyvitamin D
dc.subject.otherIndex adult
dc.subject.otherIndex article
dc.subject.otherIndex automation
dc.subject.otherIndex chemoluminescence
dc.subject.otherIndex controlled study
dc.subject.otherIndex female
dc.subject.otherIndex human
dc.subject.otherIndex intermethod comparison
dc.subject.otherIndex major clinical study
dc.subject.otherIndex male
dc.subject.otherIndex priority journal
dc.subject.otherIndex quality control
dc.subject.otherIndex radioimmunoassay
dc.subject.otherIndex United States
dc.subject.otherIndex vitamin blood level
dc.subject.otherIndex vitamin D deficiency
dc.subject.otherIndex Decision Making
dc.subject.otherIndex Female
dc.subject.otherIndex Humans
dc.subject.otherIndex Immunoassay
dc.subject.otherIndex Male
dc.subject.otherIndex Reference Standards
dc.subject.otherIndex Reproducibility of Results
dc.subject.otherIndex Vitamin D
dc.subject.otherKeywordPlus TANDEM MASS-SPECTROMETRY
dc.subject.otherKeywordPlus VITAMIN-D DEFICIENCY
dc.subject.otherKeywordPlus HYPOVITAMINOSIS-D
dc.subject.otherKeywordPlus SERUM 25-HYDROXYVITAMIN-D
dc.subject.otherKeywordPlus AUTOMATED METHODS
dc.subject.otherKeywordPlus SUNNY COUNTRY
dc.subject.otherKeywordPlus ACCURACY
dc.subject.otherKeywordPlus STANDARD
dc.subject.otherKeywordPlus D-3
dc.subject.otherWOS Endocrinology and Metabolism


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