AUB ScholarWorks

Laboratory referral rates of genetic tests for thrombophilia workup in a major referral center

Show simple item record Hoteit R. Halas H. Hassanieh S. Mahfouz R.A.R.
dc.contributor.editor May-2012 2017-10-05T15:59:34Z 2017-10-05T15:59:34Z 2012
dc.identifier 10.1089/gtmb.2011.0220
dc.identifier.issn 19450265
dc.description.abstract Aims: The rate of laboratory referrals for thrombophilia patients' genetic workup was assessed and compared among the medical and surgical specialties and subspecialties at a major tertiary care center in Lebanon. Methods: DNA extraction was performed using the PEL-FREEZ extraction kit (PEL-FREEZ; DYNAL) and the Factor V, prothrombin, and methylenetetrahydrofolate reductase genotypic profiles were done using the FV-PTH-MTHFR StripAssay kit (ViennaLab) that employs a polymerase chain reaction-reverse hybridization method. A total of 2238 referred cases were analyzed. Results: Around 42.23percent of all referred cases turned out to have a thrombosis-associated mutation. Referrals from medical and surgical specialties were almost equal. In the surgical specialty, most referrals came from the department of Obstetrics and Gynecology, while in the medical speciality, most of the workup referrals originated from the Hematology-Oncology physicians. However, low referral rates were reported from the emergency department and family medicine practitioners. Conclusion: Genetic testing for thrombophilia workup is gaining more importance among the different medical and surgical specialties and is worth being introduced into the offered test lists of all established molecular diagnostics laboratories. © 2012 Mary Ann Liebert, Inc.
dc.format.extent Pages: (459-462)
dc.language English
dc.publisher NEW ROCHELLE
dc.relation.ispartof Publication Name: Genetic Testing and Molecular Biomarkers; Publication Year: 2012; Volume: 16; no. 5; Pages: (459-462);
dc.source Scopus
dc.title Laboratory referral rates of genetic tests for thrombophilia workup in a major referral center
dc.type Article
dc.contributor.affiliation Hoteit, R., Department of Pathology, American University, Beirut Medical Center, Riad El Solh 1107 2020, Beirut 11-0236, Lebanon
dc.contributor.affiliation Halas, H., Department of Pathology, American University, Beirut Medical Center, Riad El Solh 1107 2020, Beirut 11-0236, Lebanon
dc.contributor.affiliation Hassanieh, S., Department of Pathology, American University, Beirut Medical Center, Riad El Solh 1107 2020, Beirut 11-0236, Lebanon
dc.contributor.affiliation Mahfouz, R.A.R., Department of Pathology, American University, Beirut Medical Center, Riad El Solh 1107 2020, Beirut 11-0236, Lebanon
dc.contributor.authorAddress Mahfouz, R.A.R.; Department of Pathology, American University, Beirut Medical Center, Riad El Solh 1107 2020, Beirut 11-0236, Lebanon; email:
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Pathology and Laboratory Medicine;
dc.contributor.authorDepartment Pathology and Laboratory Medicine
dc.contributor.faculty Faculty of Medicine
dc.contributor.authorInitials Hoteit, R
dc.contributor.authorInitials Halas, H
dc.contributor.authorInitials Hassanieh, S
dc.contributor.authorInitials Mahfouz, RAR
dc.contributor.authorReprintAddress Mahfouz, RAR (reprint author), Amer Univ Beirut, Med Ctr, Dept Pathol and Lab Med, Riad El Solh 1107 2020, Beirut 110236, Lebanon.
dc.contributor.authorUniversity American University of Beirut Medical Center
dc.description.cited Ayadurai T, 2009, J OBSTET GYNAECOL RE, V35, P1061, DOI 10.1111-j.1447-0756.2009.01067.x; Cohn DM, 2010, J THROMB HAEMOST, V8, P513, DOI 10.1111-j.1538-7836.2009.03710.x; Finan RR, 2002, AM J HEMATOL, V71, P300, DOI 10.1002-ajh.10223; Hussein AS, 2010, THROMB RES, V126, pE78, DOI 10.1016-j.thromres.2010.04.017; Isma'eel H, 2006, J THROMB THROMBOLYS, V22, P121, DOI 10.1007-s11239-006-8953-3; Isma'eel H, 2006, J THROMB THROMBOLYS, V21, P267, DOI 10.1007-s11239-006-5537-1; Jadaon MM, 2010, DIAGN MOL PATHOL, V19, P180; Kfoury E, 2009, MOL BIOL REP, V36, P1041, DOI 10.1007-s11033-008-9278-4; Kosar A, 2011, BLOOD COAGUL FIBRIN, V22, P14, DOI 10.1097-MBC.0b013e32834013f2; Larciprete G, 2010, J OBSTET GYNAECOL RE, V36, P996, DOI 10.1111-j.1447-0756.2010.01262.x; Lund M, 2010, HUM REPROD, V25, P2978, DOI 10.1093-humrep-deq280; Mazoyer E, 2009, BLOOD COAGUL FIBRIN, V20, P503, DOI 10.1097-MBC.0b013e32832f5d7a; Otrock ZK, 2008, ANN HEMATOL, V87, P1013, DOI 10.1007-s00277-008-0543-3; Rahimi Z, 2010, BLOOD COAGUL FIBRIN, V21, P385, DOI 10.1097-MBC.0b013e328330e69a; Sabbagh A, 2009, MOL BIOL REP, V36, P399, DOI 10.1007-s11033-007-9193-0; Tug E, 2011, INTERNAL MED, V50, P17, DOI 10.2169-internalmedicine.50.4144; Yokus O, 2009, INT J HEMATOL, V90, P583, DOI 10.1007-s12185-009-0447-6; Zahed LF, 2006, AM J OBSTET GYNECOL, V195, P1114, DOI 10.1016-j.ajog.2006.06.082
dc.description.citedCount 1
dc.description.citedTotWOSCount 1
dc.description.citedWOSCount 1
dc.format.extentCount 4
dc.identifier.pubmedID 22191678
dc.identifier.scopusID 84861524589
dc.publisher.address 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA
dc.relation.ispartOfISOAbbr Genet. Test. Mol. Biomark.
dc.relation.ispartOfIssue 5
dc.relation.ispartofPubTitle Genetic Testing and Molecular Biomarkers
dc.relation.ispartofPubTitleAbbr Gent. Test. and Mol. Biomarkers
dc.relation.ispartOfVolume 16
dc.source.ID WOS:000304448300029
dc.type.publication Journal
dc.subject.otherChemCAS 5,10 methylenetetrahydrofolate reductase (FADH2), 9028-69-7
dc.subject.otherChemCAS blood clotting factor 5, 9001-24-5, 9013-23-4
dc.subject.otherChemCAS prothrombin, 9001-26-7
dc.subject.otherChemCAS Factor V, 9001-24-5
dc.subject.otherChemCAS MTHFR protein, human,
dc.subject.otherChemCAS Methylenetetrahydrofolate Reductase (NADPH2),
dc.subject.otherChemCAS Prothrombin, 9001-26-7
dc.subject.otherIndex 5,10 methylenetetrahydrofolate reductase (FADH2)
dc.subject.otherIndex blood clotting factor 5
dc.subject.otherIndex prothrombin
dc.subject.otherIndex article
dc.subject.otherIndex DNA extraction
dc.subject.otherIndex DNA hybridization
dc.subject.otherIndex emergency care
dc.subject.otherIndex emergency medicine
dc.subject.otherIndex enzyme substrate
dc.subject.otherIndex family medicine
dc.subject.otherIndex gene mutation
dc.subject.otherIndex general surgery
dc.subject.otherIndex genetic association
dc.subject.otherIndex genetic screening
dc.subject.otherIndex genotype
dc.subject.otherIndex human
dc.subject.otherIndex laboratory test
dc.subject.otherIndex mutational analysis
dc.subject.otherIndex patient referral
dc.subject.otherIndex polymerase chain reaction
dc.subject.otherIndex tertiary health care
dc.subject.otherIndex thrombophilia
dc.subject.otherIndex vascular surgery
dc.subject.otherIndex Academic Medical Centers
dc.subject.otherIndex Factor V
dc.subject.otherIndex Female
dc.subject.otherIndex Genetic Testing
dc.subject.otherIndex Genotype
dc.subject.otherIndex Humans
dc.subject.otherIndex Male
dc.subject.otherIndex Medicine
dc.subject.otherIndex Methylenetetrahydrofolate Reductase (NADPH2)
dc.subject.otherIndex Mutation
dc.subject.otherIndex Polymerase Chain Reaction
dc.subject.otherIndex Prothrombin
dc.subject.otherIndex Referral and Consultation
dc.subject.otherIndex Specialties, Surgical
dc.subject.otherIndex Thrombophilia
dc.subject.otherKeywordPlus FACTOR-V-LEIDEN
dc.subject.otherKeywordPlus ADVERSE PREGNANCY OUTCOMES
dc.subject.otherKeywordPlus INHERITED THROMBOPHILIA
dc.subject.otherKeywordPlus VENOUS THROMBOSIS
dc.subject.otherKeywordPlus POPULATION
dc.subject.otherKeywordPlus PREVALENCE
dc.subject.otherKeywordPlus PROTHROMBIN
dc.subject.otherKeywordPlus MUTATIONS
dc.subject.otherKeywordPlus WOMEN
dc.subject.otherKeywordPlus THROMBOEMBOLISM
dc.subject.otherWOS Biochemistry and Molecular Biology
dc.subject.otherWOS Genetics and Heredity

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search AUB ScholarWorks


My Account