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Molecular frequency of BCL2-JH t(14; 18) using PCR among lebanese patients with follicular lymphoma: Another piece of the geographical map revealed

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dc.contributor.author Mahfouz R.
dc.contributor.author Shammaa D.
dc.contributor.author Tawil A.
dc.contributor.author Zaatari G.
dc.contributor.editor
dc.date Dec-2007
dc.date.accessioned 2017-10-05T15:59:45Z
dc.date.available 2017-10-05T15:59:45Z
dc.date.issued 2007
dc.identifier 10.1007/s11033-006-9042-6
dc.identifier.isbn
dc.identifier.issn 03014851
dc.identifier.uri http://hdl.handle.net/10938/19222
dc.description.abstract In follicular lymphoma the frequency of translocation t(14;18) varies considerably across different geographic regions ranging from up to 89percent among the American follicular lymphoma to around 30percent in the Japanese lymphoma. Neighboring and regional countries varied in their frequency reporting like in Israel (22 of 36 cases; 61percent), Turkey (46 of 67 cases; 68.7percent), and Jordan (4 of 5 cases; 80percent). To our knowledge, this is the first study conducted in Lebanon to determine the frequency of this translocation in follicular lymphoma patients. Of 42 cases diagnosed with follicular lymphoma at the American University of Beirut Medical Center, amplifiable DNA was extracted from the corresponding paraffin embedded tissues and tested for t(14; 18) translocation using PCR amplification of the MBR and MCR breakpoints (INVIVOSCRIBE, CA, USA). We found that 19 patients were positive for t(14; 18) (45.2percent) while 23 were negative (54.8percent). Among the 19 positive cases, bcl2 was positive in 10 cases (52.6percent). The majority of the cases were positive for MBR (40.47percent), while only two cases were positive for MCR (4.76percent). This study expands the geographical map of the distribution of bcl-2 gene rearrangement in follicular lymphoma patients in the Middle East region. The interesting low frequency of t(14;18) in Lebanese follicular lymphoma patients (45.2percent) stands out among several other increased frequencies in surrounding and regional countries. In addition, in this patient population, there is a decreased frequency of the MBR breakpoint (40.47percent) while that reported in the literature ranges from 50 to 60percent. © 2006 Springer Science+Business Media, Inc.
dc.format.extent
dc.format.extent Pages: (271-274)
dc.language English
dc.publisher DORDRECHT
dc.relation.ispartof Publication Name: Molecular Biology Reports; Publication Year: 2007; Volume: 34; no. 4; Pages: (271-274);
dc.relation.ispartofseries
dc.relation.uri
dc.source Scopus
dc.subject.other
dc.title Molecular frequency of BCL2-JH t(14; 18) using PCR among lebanese patients with follicular lymphoma: Another piece of the geographical map revealed
dc.type Article
dc.contributor.affiliation Mahfouz, R., Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, P.O. Box 11-0236, Riad El Solh 1107 2020, Beirut, Lebanon
dc.contributor.affiliation Shammaa, D., Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, P.O. Box 11-0236, Riad El Solh 1107 2020, Beirut, Lebanon
dc.contributor.affiliation Tawil, A., Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, P.O. Box 11-0236, Riad El Solh 1107 2020, Beirut, Lebanon
dc.contributor.affiliation Zaatari, G., Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, P.O. Box 11-0236, Riad El Solh 1107 2020, Beirut, Lebanon
dc.contributor.authorAddress Mahfouz, R.; Department of Pathology and Laboratory Medicine, American University of Beirut Medical Center, P.O. Box 11-0236, Riad El Solh 1107 2020, Beirut, Lebanon; email: rm11@aub.edu.lb
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Pathology and Laboratory Medicine;
dc.contributor.authorDepartment Pathology and Laboratory Medicine
dc.contributor.authorDivision
dc.contributor.authorEmail rm11@aub.edu.lb
dc.contributor.authorFaculty Faculty of Medicine
dc.contributor.authorInitials Mahfouz, R
dc.contributor.authorInitials Shammaa, D
dc.contributor.authorInitials Tawil, A
dc.contributor.authorInitials Zaatari, G
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Mahfouz, R (reprint author), Amer Univ Beirut, Med Ctr, Dept Pathol and Lab Med, Riad El Sohl 1107 2020,PO Box 11-0236, Beirut, Lebanon.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut Medical Center
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dc.description.citedCount 5
dc.description.citedTotWOSCount 3
dc.description.citedWOSCount 3
dc.format.extentCount 4
dc.identifier.articleNo
dc.identifier.coden MLBRB
dc.identifier.pubmedID 17149654
dc.identifier.scopusID 35448947224
dc.identifier.url
dc.publisher.address VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
dc.relation.ispartofConference
dc.relation.ispartofConferenceCode
dc.relation.ispartofConferenceDate
dc.relation.ispartofConferenceHosting
dc.relation.ispartofConferenceLoc
dc.relation.ispartofConferenceSponsor
dc.relation.ispartofConferenceTitle
dc.relation.ispartofFundingAgency
dc.relation.ispartOfISOAbbr Mol. Biol. Rep.
dc.relation.ispartOfIssue 4
dc.relation.ispartOfPart
dc.relation.ispartofPubTitle Molecular Biology Reports
dc.relation.ispartofPubTitleAbbr Mol. Biol. Rep.
dc.relation.ispartOfSpecialIssue
dc.relation.ispartOfSuppl
dc.relation.ispartOfVolume 34
dc.source.ID WOS:000250380300010
dc.type.publication Journal
dc.subject.otherAuthKeyword bcl2-JH
dc.subject.otherAuthKeyword Distribution
dc.subject.otherAuthKeyword Follicular
dc.subject.otherAuthKeyword Lebanon
dc.subject.otherAuthKeyword Lymphoma
dc.subject.otherChemCAS protein bcl 2, 219306-68-0
dc.subject.otherChemCAS Immunoglobulin Joining Region
dc.subject.otherChemCAS Proto-Oncogene Proteins c-bcl-2
dc.subject.otherIndex breakpoint cluster region protein
dc.subject.otherIndex minor cluster region protein
dc.subject.otherIndex paraffin
dc.subject.otherIndex protein bcl 2
dc.subject.otherIndex unclassified drug
dc.subject.otherIndex article
dc.subject.otherIndex clinical article
dc.subject.otherIndex controlled study
dc.subject.otherIndex DNA extraction
dc.subject.otherIndex embedding
dc.subject.otherIndex follicular lymphoma
dc.subject.otherIndex gene amplification
dc.subject.otherIndex gene cluster
dc.subject.otherIndex gene frequency
dc.subject.otherIndex gene translocation
dc.subject.otherIndex geographic distribution
dc.subject.otherIndex human
dc.subject.otherIndex Israel
dc.subject.otherIndex Jordan
dc.subject.otherIndex Lebanon
dc.subject.otherIndex polymerase chain reaction
dc.subject.otherIndex Turkey (republic)
dc.subject.otherIndex Chromosomes, Human, Pair 14
dc.subject.otherIndex Chromosomes, Human, Pair 18
dc.subject.otherIndex Gene Frequency
dc.subject.otherIndex Humans
dc.subject.otherIndex Immunoglobulin Joining Region
dc.subject.otherIndex Lebanon
dc.subject.otherIndex Lymphoma, Follicular
dc.subject.otherIndex Polymerase Chain Reaction
dc.subject.otherIndex Proto-Oncogene Proteins c-bcl-2
dc.subject.otherIndex Translocation, Genetic
dc.subject.otherKeywordPlus POLYMERASE-CHAIN-REACTION
dc.subject.otherKeywordPlus BCL-2 GENE REARRANGEMENT
dc.subject.otherKeywordPlus CHROMOSOMAL TRANSLOCATION
dc.subject.otherKeywordPlus CLUSTER REGION
dc.subject.otherKeywordPlus T(14-18)
dc.subject.otherKeywordPlus CLASSIFICATION
dc.subject.otherKeywordPlus TISSUES
dc.subject.otherWOS Biochemistry and Molecular Biology


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