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Epinephrine deficiency results in intact glucose counter-regulation, severe hepatic steatosis and possible defective autophagy in fasting mice

Show simple item record Sharara-Chami R.I. Zhou Y. Ebert S. Pacak K. Ozcan U. Majzoub J.A.
dc.contributor.editor Jun-2012 2017-10-05T16:01:16Z 2017-10-05T16:01:16Z 2012
dc.identifier 10.1016/j.biocel.2012.02.016
dc.identifier.issn 13572725
dc.description.abstract Epinephrine is one of the major hormones involved in glucose counter-regulation and gluconeogenesis. However, little is known about its importance in energy homeostasis during fasting. Our objective is to study the specific role of epinephrine in glucose and lipid metabolism during starvation. In our experiment, we subject regular mice and epinephrine-deficient mice to a 48-h fast then we evaluate the different metabolic responses to fasting. Our results show that epinephrine is not required for glucose counter-regulation: epinephrine-deficient mice maintain their blood glucose at normal fasting levels via glycogenolysis and gluconeogenesis, with normal fasting-induced changes in the peroxisomal activators: peroxisome proliferator activated receptor γ coactivator α (PGC-1α), fibroblast growth factor 21 (FGF-21), peroxisome proliferator activated receptor α (PPAR-α), and sterol regulatory element binding protein (SREBP-1c). However, fasted epinephrine-deficient mice develop severe ketosis and hepatic steatosis, with evidence for inhibition of hepatic autophagy, a process that normally provides essential energy via degradation of hepatic triglycerides during starvation. We conclude that, during fasting, epinephrine is not required for glucose homeostasis, lipolysis or ketogenesis. Epinephrine may have an essential role in lipid handling, possibly via an autophagy-dependent mechanism. © 2012 Elsevier Ltd.
dc.format.extent Pages: (905-913)
dc.language English
dc.publisher OXFORD
dc.relation.ispartof Publication Name: International Journal of Biochemistry and Cell Biology; Publication Year: 2012; Volume: 44; no. 6; Pages: (905-913);
dc.source Scopus
dc.title Epinephrine deficiency results in intact glucose counter-regulation, severe hepatic steatosis and possible defective autophagy in fasting mice
dc.type Article
dc.contributor.affiliation Sharara-Chami, R.I., Department of Anesthesiology, Harvard Medical School, Children's Hospital Boston, Boston, MA 02115, United States, Department of Pediatrics and Adolescent Medicine, Beirut Medical Center, American University, Beirut, Lebanon
dc.contributor.affiliation Zhou, Y., Department of Medicine, Harvard Medical School, Children's Hospital Boston, Boston, MA 02115, United States
dc.contributor.affiliation Ebert, S., Biomedical Sciences Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, Orlando, FL 32816, United States
dc.contributor.affiliation Pacak, K., Reproductive Biology and Medicine Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, United States
dc.contributor.affiliation Ozcan, U., Department of Medicine, Harvard Medical School, Children's Hospital Boston, Boston, MA 02115, United States
dc.contributor.affiliation Majzoub, J.A., Department of Medicine, Harvard Medical School, Children's Hospital Boston, Boston, MA 02115, United States
dc.contributor.authorAddress Sharara-Chami, R.I.; Department of Pediatrics and Adolescent Medicine, Beirut Medical Center, American University, Beirut, Lebanon; email:
dc.contributor.authorCorporate University: American University of Beirut Medical Center; Faculty: Faculty of Medicine; Department: Pediatrics and Adolescent Medicine;
dc.contributor.authorDepartment Pediatrics and Adolescent Medicine
dc.contributor.faculty Faculty of Medicine
dc.contributor.authorInitials Sharara-Chami, RI
dc.contributor.authorInitials Zhou, YJ
dc.contributor.authorInitials Ebert, S
dc.contributor.authorInitials Pacak, K
dc.contributor.authorInitials Ozcan, U
dc.contributor.authorInitials Majzoub, JA
dc.contributor.authorReprintAddress Sharara-Chami, RI (reprint author), Amer Univ Beirut, Med Ctr, Dept Pediat and Adolescent Med, Div Pediat Crit Care Med, Beirut, Lebanon.
dc.contributor.authorUniversity American University of Beirut Medical Center
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dc.description.citedCount 4
dc.description.citedTotWOSCount 6
dc.description.citedWOSCount 6
dc.format.extentCount 9
dc.identifier.coden IJBBF
dc.identifier.pubmedID 22405854
dc.identifier.scopusID 84862827930
dc.relation.ispartOfISOAbbr Int. J. Biochem. Cell Biol.
dc.relation.ispartOfIssue 6
dc.relation.ispartofPubTitle International Journal of Biochemistry and Cell Biology
dc.relation.ispartofPubTitleAbbr Int. J. Biochem. Cell Biol.
dc.relation.ispartOfVolume 44
dc.source.ID WOS:000304490700013
dc.type.publication Journal
dc.subject.otherAuthKeyword Autophagy
dc.subject.otherAuthKeyword Brown adipose tissue
dc.subject.otherAuthKeyword Epinephrine
dc.subject.otherAuthKeyword Fasting
dc.subject.otherAuthKeyword Metabolism
dc.subject.otherAuthKeyword Steatosis
dc.subject.otherChemCAS adrenalin, 51-43-4, 55-31-2, 6912-68-1
dc.subject.otherChemCAS glucose, 50-99-7, 84778-64-3
dc.subject.otherChemCAS peroxisome proliferator activated receptor alpha, 147258-70-6
dc.subject.otherChemCAS Blood Glucose
dc.subject.otherChemCAS DNA Primers
dc.subject.otherChemCAS Epinephrine, 51-43-4
dc.subject.otherChemCAS Liver Glycogen
dc.subject.otherIndex adrenalin
dc.subject.otherIndex fibroblast growth factor 21
dc.subject.otherIndex glucose
dc.subject.otherIndex peroxisome proliferator activated receptor alpha
dc.subject.otherIndex peroxisome proliferator activated receptor gamma
dc.subject.otherIndex sterol regulatory element binding protein 1c
dc.subject.otherIndex triacylglycerol
dc.subject.otherIndex amino acid sequence
dc.subject.otherIndex animal experiment
dc.subject.otherIndex animal model
dc.subject.otherIndex animal tissue
dc.subject.otherIndex article
dc.subject.otherIndex autophagy
dc.subject.otherIndex diet restriction
dc.subject.otherIndex fatty liver
dc.subject.otherIndex gluconeogenesis
dc.subject.otherIndex glucose blood level
dc.subject.otherIndex glycogenolysis
dc.subject.otherIndex ketoacidosis
dc.subject.otherIndex lipid metabolism
dc.subject.otherIndex male
dc.subject.otherIndex mouse
dc.subject.otherIndex nonhuman
dc.subject.otherIndex Absorptiometry, Photon
dc.subject.otherIndex Animals
dc.subject.otherIndex Autophagy
dc.subject.otherIndex Base Sequence
dc.subject.otherIndex Blood Glucose
dc.subject.otherIndex Blotting, Western
dc.subject.otherIndex DNA Primers
dc.subject.otherIndex Epinephrine
dc.subject.otherIndex Fasting
dc.subject.otherIndex Fatty Liver
dc.subject.otherIndex Liver Glycogen
dc.subject.otherIndex Male
dc.subject.otherIndex Mice
dc.subject.otherIndex Reverse Transcriptase Polymerase Chain Reaction
dc.subject.otherIndex Mus
dc.subject.otherKeywordPlus ADRENERGIC-MECHANISMS
dc.subject.otherKeywordPlus GLYCEMIC THRESHOLDS
dc.subject.otherKeywordPlus CELLULAR AUTOPHAGY
dc.subject.otherKeywordPlus INSULIN-RESISTANCE
dc.subject.otherKeywordPlus RAT LIVER
dc.subject.otherKeywordPlus GLUCAGON
dc.subject.otherKeywordPlus DISEASE
dc.subject.otherKeywordPlus HUMANS
dc.subject.otherKeywordPlus ALPHA
dc.subject.otherKeywordPlus COUNTERREGULATION
dc.subject.otherWOS Biochemistry and Molecular Biology
dc.subject.otherWOS Cell Biology

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