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Detection and quantitation of the new world Squash leaf curl virus by TaqMan real-time PCR

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dc.contributor.author Abrahamian P.E.
dc.contributor.author Abou-Jawdah Y.
dc.contributor.editor
dc.date 2013
dc.date.accessioned 2017-10-18T13:13:43Z
dc.date.available 2017-10-18T13:13:43Z
dc.date.issued 2013
dc.identifier 10.1016/j.jviromet.2013.04.001
dc.identifier.issn 1660934
dc.identifier.uri http://hdl.handle.net/10938/20176
dc.description.abstract Squash leaf curl diseases are caused by distinct virus species that are separated into two major phylogenetic groups, western and eastern hemisphere groups. The western group includes the new world Squash leaf curl virus (SLCV) which causes major losses to cucurbit production and induces severe stunting and leaf curl in squash plants. A TaqMan-based real time polymerase chain reaction (qPCR) assay has been developed for detection and quantitation of SLCV. Designed primers and probe targeted the AV1 (coat protein) gene and in silico analysis showed that they detect a large number of SLCV isolates. The developed assay could detect the virus in 18. fg of total nucleic acid and 30 genomic units. The qPCR assay was about 1000 times more sensitive than PCR and amplified successfully SLCV from a wide range of cucurbit hosts and from viruliferous whiteflies. The developed qPCR assay should be suitable for detection and quantitation purposes for all reported SLCV isolates of the western hemisphere. © 2013 Elsevier B.V.
dc.format.extent
dc.format.extent Pages: (76-81)
dc.language English
dc.publisher AMSTERDAM
dc.relation.ispartof Publication Name: Journal of Virological Methods; Publication Year: 2013; Volume: 191; no. 1; Pages: (76-81);
dc.source Scopus
dc.subject.other Genbank: af173555, af256200, af256203, af325497, af414287, af421552, af509739, af509743, aj420319, am260699, ay742220, dq285016, dq285019, dq641694, dq866135, ef197940, ef199774, ef532620, eu310406, eu479710, eu487031, eu487033, gq273922, hm368371, hm3683
dc.title Detection and quantitation of the new world Squash leaf curl virus by TaqMan real-time PCR
dc.type Article
dc.contributor.affiliation Abrahamian, P.E., Department of Agricultural Sciences, Faculty of Agricultural and Food Sciences, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Abou-Jawdah, Y., Department of Agricultural Sciences, Faculty of Agricultural and Food Sciences, American University of Beirut, Beirut 1107 2020, Lebanon
dc.contributor.authorAddress Abou-Jawdah, Y.; Department of Agricultural Sciences, Faculty of Agricultural and Food Sciences, American University of Beirut, Beirut 1107 2020, Lebanon; email: abujawyf@aub.edu.lb
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Agricultural and Food Sciences; Department: Agricultural Sciences;
dc.contributor.authorDepartment Agricultural Sciences
dc.contributor.authorDivision
dc.contributor.authorEmail abujawyf@aub.edu.lb
dc.contributor.authorFaculty Faculty of Agricultural and Food Sciences
dc.contributor.authorInitials Abrahamian, PE
dc.contributor.authorInitials Abou-Jawdah, Y
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Abou-Jawdah, Y (reprint author), Amer Univ Beirut, Dept Agr Sci, Fac Agr and Food Sci, Beirut 11072020, Lebanon.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut
dc.description.cited Ali-Shtayeh MS, 2010, PLANT DIS, V94, P640, DOI 10.1094-PDIS-94-5-0640B; Al-Musa A, 2008, J PHYTOPATHOL, V156, P311, DOI 10.1111-j.1439-0434.2007.01389.x; Antignus Y, 2003, PHYTOPARASITICA, V31, P415; Brown JK, 2012, VIRUS TAXONOMY, P351; CACIAGLI P, 1995, EUR J PLANT PATHOL, V101, P163, DOI 10.1007-BF01874762; Caciagli P, 1997, PHYTOPATHOLOGY, V87, P610, DOI 10.1094-PHYTO.1997.87.6.610; COHEN S, 1983, PHYTOPATHOLOGY, V73, P1669, DOI 10.1094-Phyto-73-1669; FLOCK RA, 1981, PLANT DIS, V65, P75; Idris AM, 2006, PLANT DIS, V90, P1262, DOI 10.1094-PD-90-1262B; Kuan CP, 2012, J VIROL METHODS, V179, P367, DOI 10.1016-j.jviromet.2011.11.024; LAZAROWITZ SG, 1991, VIROLOGY, V180, P58, DOI 10.1016-0042-6822(91)90009-Z; LAZAROWITZ SG, 1991, VIROLOGY, V180, P70, DOI 10.1016-0042-6822(91)90010-9; Mason G, 2008, J VIROL METHODS, V147, P282, DOI 10.1016-j.jviromet.2007.09.015; POLSTON JE, 1989, PHYTOPATHOLOGY, V79, P1123, DOI 10.1094-Phyto-79-1123; Sobh H, 2012, PLANT DIS, V96, P1231, DOI 10.1094-PDIS-04-12-0365-PDN; Sufrin-Ringwald T, 2011, PHYTOPATHOLOGY, V101, P281, DOI 10.1094-PHYTO-06-10-0159
dc.description.citedCount
dc.description.citedTotWOSCount 1
dc.description.citedWOSCount 0
dc.format.extentCount 6
dc.identifier.articleNo
dc.identifier.coden JVMED
dc.identifier.pubmedID 23583490
dc.identifier.scopusID 84877339144
dc.publisher.address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
dc.relation.ispartOfISOAbbr J. Virol. Methods
dc.relation.ispartOfIssue 1
dc.relation.ispartofPubTitle Journal of Virological Methods
dc.relation.ispartofPubTitleAbbr J. Virol. Methods
dc.relation.ispartOfVolume 191
dc.source.ID WOS:000320217100012
dc.type.publication Journal
dc.subject.otherAuthKeyword Begomovirus
dc.subject.otherAuthKeyword cucurbits
dc.subject.otherAuthKeyword qpcr
dc.subject.otherAuthKeyword slcv
dc.subject.otherAuthKeyword whitefly
dc.subject.otherChemCAS Dna primers
dc.subject.otherChemCAS oligonucleotide probes
dc.subject.otherIndex Coat protein
dc.subject.otherIndex article
dc.subject.otherIndex av1 gene
dc.subject.otherIndex begomovirus
dc.subject.otherIndex computer analysis
dc.subject.otherIndex controlled study
dc.subject.otherIndex gene targeting
dc.subject.otherIndex nonhuman
dc.subject.otherIndex nucleic acid amplification
dc.subject.otherIndex nucleotide sequence
dc.subject.otherIndex phylogeny
dc.subject.otherIndex polymerase chain reaction
dc.subject.otherIndex priority journal
dc.subject.otherIndex real time polymerase chain reaction
dc.subject.otherIndex sensitivity analysis
dc.subject.otherIndex squash
dc.subject.otherIndex squash leaf curl virus
dc.subject.otherIndex virus detection
dc.subject.otherIndex virus gene
dc.subject.otherIndex virus isolation
dc.subject.otherIndex virus titration
dc.subject.otherIndex western hemisphere
dc.subject.otherIndex whitefly
dc.subject.otherIndex animals
dc.subject.otherIndex begomovirus
dc.subject.otherIndex cucurbita
dc.subject.otherIndex dna primers
dc.subject.otherIndex hemiptera
dc.subject.otherIndex oligonucleotide probes
dc.subject.otherIndex plant diseases
dc.subject.otherIndex real-time polymerase chain reaction
dc.subject.otherIndex sensitivity and specificity
dc.subject.otherIndex virology
dc.subject.otherKeywordPlus Molecular characterization
dc.subject.otherKeywordPlus whitefly vector
dc.subject.otherKeywordPlus cucurbita-pepo
dc.subject.otherKeywordPlus bemisia-tabaci
dc.subject.otherKeywordPlus host-range
dc.subject.otherKeywordPlus 1st report
dc.subject.otherKeywordPlus geminivirus
dc.subject.otherKeywordPlus disease
dc.subject.otherWOS Biochemical research methods
dc.subject.otherWOS biotechnology and applied microbiology
dc.subject.otherWOS virology
dc.identifier.doi http://dx.doi.org/10.1016/j.jviromet.2013.04.001


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