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Improving biological relevancy of transcriptional biomarkers experiments by applying the MIQE guidelines to pre-clinical and clinical trials

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dc.contributor.author Dooms M.
dc.contributor.author Chango A.
dc.contributor.author Barbour E.
dc.contributor.author Pouillart P.
dc.contributor.author Abdel Nour A.M.
dc.contributor.editor
dc.date 2013
dc.date.accessioned 2017-10-18T13:27:36Z
dc.date.available 2017-10-18T13:27:36Z
dc.date.issued 2013
dc.identifier 10.1016/j.ymeth.2012.07.024
dc.identifier.issn 10462023
dc.identifier.uri http://hdl.handle.net/10938/20368
dc.description.abstract The Minimum Information for the Publication of qPCR Experiments (MIQE [3]) guidelines are very much targeted at basic research experiments and have to our knowledge not been applied to qPCR assays carried out in the context of clinical trials. This report details the use of the MIQE qPCR app for iPhone (App Store, Apple) to assess the MIQE compliance of one clinical and five pre-clinical trials. This resulted in the need to include 14 modifications that make the guidelines more relevant for the assessment of this special type of application. We also discuss the need for flexibility, since while some parameters increase experimental quality, they also require more reagents and more time, which is not always feasible in a clinical setting. © 2012.
dc.format.extent
dc.format.extent Pages: (147-153)
dc.language English
dc.publisher SAN DIEGO
dc.relation.ispartof Publication Name: Methods; Publication Year: 2013; Volume: 59; no. 1; Pages: (147-153);
dc.source Scopus
dc.subject.other GENBANK: GU119892, NM_204267
dc.title Improving biological relevancy of transcriptional biomarkers experiments by applying the MIQE guidelines to pre-clinical and clinical trials
dc.type Article
dc.contributor.affiliation Dooms, M., LaSalle Beauvais, 19 rue Pierre Waguet, 60 000 Beauvais, France
dc.contributor.affiliation Chango, A., LaSalle Beauvais, 19 rue Pierre Waguet, 60 000 Beauvais, France
dc.contributor.affiliation Barbour, E., Department of Animal and Veterinary Sciences, American University of Beirut, P.O. Box 11-0236, Riad El-Solh, Beirut 1107 2020, Lebanon
dc.contributor.affiliation Pouillart, P., LaSalle Beauvais, 19 rue Pierre Waguet, 60 000 Beauvais, France
dc.contributor.affiliation Abdel Nour, A.M., LaSalle Beauvais, 19 rue Pierre Waguet, 60 000 Beauvais, France
dc.contributor.authorAddress Abdel Nour, A.M.; LaSalle Beauvais, 19 rue Pierre Waguet, 60 000 Beauvais, France; email: afif.abdelnour@gmail.com
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Agricultural and Food Sciences; Department: Animal and Veterinary Sciences;
dc.contributor.authorDepartment Animal and Veterinary Sciences
dc.contributor.authorDivision
dc.contributor.authorEmail afif.abdelnour@gmail.com
dc.contributor.authorFaculty Faculty of Agricultural and Food Sciences
dc.contributor.authorInitials Dooms, M
dc.contributor.authorInitials Chango, A
dc.contributor.authorInitials Barbour, E
dc.contributor.authorInitials Pouillart, P
dc.contributor.authorInitials Nour, AMA
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Nour, AMA (reprint author), LaSalle Beauvais, 19 Rue Pierre Waguet, F-60000 Beauvais, France.
dc.contributor.authorResearcherID Abdel Nour, Afif-D-4003-2013; barbour, elie-P-6166-2014
dc.contributor.authorUniversity American University of Beirut
dc.description.cited Abdel Nour AM, 2010, EUR PHARM REV DIG, V15, P31; Bustin SA, 2009, CLIN CHEM, V55, P611, DOI 10.1373-clinchem.2008.112797; Huggett J., 2010, EUR PHARM REV, V15, P17; Lecerf J.M., 2012, BR J NUTR, P1; Pfaffl M.W., 2011, APP STORE; Taylor CF, 2008, NAT BIOTECHNOL, V26, P889, DOI 10.1038-nbt.1411
dc.description.citedCount 4
dc.description.citedTotWOSCount 5
dc.description.citedWOSCount 5
dc.format.extentCount 7
dc.identifier.articleNo
dc.identifier.coden MTHDE
dc.identifier.pubmedID 22910527
dc.identifier.scopusID 84872182585
dc.publisher.address 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
dc.relation.ispartOfISOAbbr Methods
dc.relation.ispartOfIssue 1
dc.relation.ispartofPubTitle Methods
dc.relation.ispartofPubTitleAbbr Methods
dc.relation.ispartOfVolume 59
dc.source.ID WOS:000314555700017
dc.type.publication Journal
dc.subject.otherAuthKeyword Clinical trial
dc.subject.otherAuthKeyword miqe qpcr
dc.subject.otherAuthKeyword transcriptional biomarkers
dc.subject.otherChemCAS Biological markers
dc.subject.otherChemCAS rna, messenger
dc.subject.otherIndex Biological marker
dc.subject.otherIndex animal experiment
dc.subject.otherIndex animal tissue
dc.subject.otherIndex article
dc.subject.otherIndex chicken
dc.subject.otherIndex clinical trial (topic)
dc.subject.otherIndex controlled study
dc.subject.otherIndex human
dc.subject.otherIndex immune response
dc.subject.otherIndex inflammation
dc.subject.otherIndex molecular biology
dc.subject.otherIndex nonhuman
dc.subject.otherIndex nucleotide sequence
dc.subject.otherIndex polymerase chain reaction
dc.subject.otherIndex practice guideline
dc.subject.otherIndex priority journal
dc.subject.otherIndex rabbit
dc.subject.otherIndex reliability
dc.subject.otherIndex swine
dc.subject.otherIndex th1 cell
dc.subject.otherIndex th2 cell
dc.subject.otherIndex turkey (bird)
dc.subject.otherIndex animals
dc.subject.otherIndex biological markers
dc.subject.otherIndex chickens
dc.subject.otherIndex clinical trials as topic
dc.subject.otherIndex gene expression profiling
dc.subject.otherIndex humans
dc.subject.otherIndex practice guidelines as topic
dc.subject.otherIndex rabbits
dc.subject.otherIndex real-time polymerase chain reaction
dc.subject.otherIndex reverse transcriptase polymerase chain reaction
dc.subject.otherIndex rna, messenger
dc.subject.otherIndex software
dc.subject.otherIndex sus scrofa
dc.subject.otherIndex transcription, genetic
dc.subject.otherIndex turkey
dc.subject.otherIndex malus x domestica
dc.subject.otherWOS Biochemical Research Methods
dc.subject.otherWOS Biochemistry and Molecular Biology
dc.identifier.doi http://dx.doi.org/10.1016/j.ymeth.2012.07.024


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