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Effects of benzo-a-pyrene on oxytocin-induced Ca2+ oscillations in myometrial cells

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dc.contributor.author Barhoumi R.
dc.contributor.author Awooda I.
dc.contributor.author Mouneimne Y.
dc.contributor.author Safe S.
dc.contributor.author Burghardt R.C.
dc.contributor.editor
dc.date 2006
dc.date.accessioned 2017-10-18T13:27:42Z
dc.date.available 2017-10-18T13:27:42Z
dc.date.issued 2006
dc.identifier 10.1016/j.toxlet.2006.02.005
dc.identifier.issn 3784274
dc.identifier.uri http://hdl.handle.net/10938/20400
dc.description.abstract Benzo-a-pyrene (BaP) is a polycyclic aromatic hydrocarbon that exists as a major environmental pollutant. The effect of this carcinogen-mutagen upon myometrial Ca2+ signaling in a human myometrial cell line (PHM1) was examined. Exposure of cells to BaP did not alter basal Ca2+ levels or the inositol(1,4,5) trisphosphate-releasable Ca2+ pool. However, BaP significantly decreased the initial oxytocin-induced Ca2+ transient and the frequency of oxytocin-induced Ca2+oscillations as well as delayed their onset. To determine the specific effects of BaP, pharmacologic agents that target intracellular Ca2+ homeostasis mechanisms were used. Genistein (a non-specific tyrosine kinase inhibitor) and AG1478 (an epidermal growth factor receptor blocker) markedly reduced the oxytocin-induced Ca2+ oscillations in control, but had no effect in BaP treated cells. Addition of epidermal growth factor or serum before or after oxytocin restored the Ca2+ oscillations in BaP treated cells to a level similar to control cells, while the K+ channel blocker tetraethylammonium chloride, partially restored the Ca2+ response. These data suggest that the tyrosine kinase pathway, which is part of the G-protein coupled receptor pathway response to oxytocin in PHM1 cells, is a target of BaP action and that EGF or serum can restore the oxytocin-induced Ca2+ oscillations. © 2006 Elsevier Ireland Ltd. All rights reserved.
dc.format.extent Pages: (133-141)
dc.language English
dc.publisher CLARE
dc.relation.ispartof Publication Name: Toxicology Letters; Publication Year: 2006; Volume: 165; no. 2; Pages: (133-141);
dc.source Scopus
dc.title Effects of benzo-a-pyrene on oxytocin-induced Ca2+ oscillations in myometrial cells
dc.type Article
dc.contributor.affiliation Barhoumi, R., Depatrment of Veterinary Integrative Biosciences, Texas A and M University, College Station, TX 77843-4458, United States
dc.contributor.affiliation Awooda, I., Depatrment of Veterinary Integrative Biosciences, Texas A and M University, College Station, TX 77843-4458, United States
dc.contributor.affiliation Mouneimne, Y., Central Research Science Laboratory, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Safe, S., Department of Veterinary Physiology and Pharmacology, Texas A and M University, College Station, TX 77843-4466, United States
dc.contributor.affiliation Burghardt, R.C., Depatrment of Veterinary Integrative Biosciences, Texas A and M University, College Station, TX 77843-4458, United States
dc.contributor.authorAddress Barhoumi, R.; Depatrment of Veterinary Integrative Biosciences, Texas A and M University, College Station, TX 77843-4458, United States; email: rmouneimne@cvm.tamu.edu
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Agricultural and Food Sciences; Department: FAFS;
dc.contributor.authorDepartment FAFS
dc.contributor.authorDivision
dc.contributor.authorEmail rmouneimne@cvm.tamu.edu
dc.contributor.authorFaculty Faculty of Agricultural and Food Sciences
dc.contributor.authorInitials Barhoumi, R
dc.contributor.authorInitials Awooda, I
dc.contributor.authorInitials Mouneimne, Y
dc.contributor.authorInitials Safe, S
dc.contributor.authorInitials Burghardt, RC
dc.contributor.authorOrcidID
dc.contributor.authorReprintAddress Barhoumi, R (reprint author), Texas AandM Univ, Dept Vet Integrat Biosci, College Stn, TX 77843 USA.
dc.contributor.authorResearcherID
dc.contributor.authorUniversity American University of Beirut
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dc.description.citedCount 18
dc.description.citedTotWOSCount 17
dc.description.citedWOSCount 17
dc.format.extentCount 9
dc.identifier.coden TOLED
dc.identifier.pubmedID 16567066
dc.identifier.scopusID 33745219175
dc.publisher.address ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND
dc.relation.ispartOfISOAbbr Toxicol. Lett.
dc.relation.ispartOfIssue 2
dc.relation.ispartofPubTitle Toxicology Letters
dc.relation.ispartofPubTitleAbbr Toxicol. Lett.
dc.relation.ispartOfVolume 165
dc.source.ID WOS:000239185100004
dc.type.publication Journal
dc.subject.otherAuthKeyword Benzo-a-pyrene
dc.subject.otherAuthKeyword calcium oscillations
dc.subject.otherAuthKeyword epidermal growth factor
dc.subject.otherAuthKeyword human myometrial cells
dc.subject.otherAuthKeyword store operated channels
dc.subject.otherAuthKeyword tyrosine kinase
dc.subject.otherChemCAS 4 (3 chloroanilino) 6,7 dimethoxyquinazoline, 153436-53-4
dc.subject.otherChemCAS benzo[a]pyrene, 50-32-8
dc.subject.otherChemCAS calcium ion, 14127-61-8
dc.subject.otherChemCAS genistein, 446-72-0
dc.subject.otherChemCAS inositol 1,4,5 trisphosphate, 85166-31-0, 88269-39-0
dc.subject.otherChemCAS oxytocin, 50-56-6, 54577-94-5
dc.subject.otherChemCAS protein tyrosine kinase, 80449-02-1
dc.subject.otherChemCAS tet
dc.subject.otherIndex 4 (3 chloroanilino) 6,7 dimethoxyquinazoline
dc.subject.otherIndex benzo[a]pyrene
dc.subject.otherIndex calcium ion
dc.subject.otherIndex carcinogen
dc.subject.otherIndex epidermal growth factor receptor kinase inhibitor
dc.subject.otherIndex g protein coupled receptor
dc.subject.otherIndex genistein
dc.subject.otherIndex inositol 1,4,5 trisphosphate
dc.subject.otherIndex mutagenic agent
dc.subject.otherIndex oxytocin
dc.subject.otherIndex potassium channel blocking agent
dc.subject.otherIndex protein tyrosine kinase
dc.subject.otherIndex protein tyrosine kinase inhibitor
dc.subject.otherIndex tetrylammonium chloride
dc.subject.otherIndex article
dc.subject.otherIndex calcium cell level
dc.subject.otherIndex cell line
dc.subject.otherIndex controlled study
dc.subject.otherIndex drug effect
dc.subject.otherIndex female
dc.subject.otherIndex homeostasis
dc.subject.otherIndex human
dc.subject.otherIndex human cell
dc.subject.otherIndex myometrium
dc.subject.otherIndex oscillation
dc.subject.otherIndex priority journal
dc.subject.otherIndex serum
dc.subject.otherIndex adult
dc.subject.otherIndex benzo(a)pyrene
dc.subject.otherIndex calcium
dc.subject.otherIndex calcium signaling
dc.subject.otherIndex carcinogens, environmental
dc.subject.otherIndex cells, cultured
dc.subject.otherIndex dose-response relationship, drug
dc.subject.otherIndex drug antagonism
dc.subject.otherIndex drug combinations
dc.subject.otherIndex enzyme inhibitors
dc.subject.otherIndex epidermal growth factor
dc.subject.otherIndex female
dc.subject.otherIndex genistein
dc.subject.otherIndex humans
dc.subject.otherIndex myometrium
dc.subject.otherIndex oxytocin
dc.subject.otherIndex pregnancy
dc.subject.otherIndex protein-tyrosine kinases
dc.subject.otherIndex tetraethylammonium
dc.subject.otherIndex tyrphostins
dc.subject.otherKeywordPlus Protein-kinase-c
dc.subject.otherKeywordPlus epidermal-growth-factor
dc.subject.otherKeywordPlus polycyclic aromatic-hydrocarbons
dc.subject.otherKeywordPlus calcium oscillations
dc.subject.otherKeywordPlus phospholipase-c
dc.subject.otherKeywordPlus tyrosine phosphorylation
dc.subject.otherKeywordPlus factor receptor
dc.subject.otherKeywordPlus muscle-cells
dc.subject.otherKeywordPlus benzo(a)pyrene
dc.subject.otherKeywordPlus metabolism
dc.subject.otherWOS Toxicology
dc.identifier.doi http://dx.doi.org/10.1016/j.toxlet.2006.02.005


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