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Hepatitis B virus DNA in serum of 'anti-HBc only'-positive healthy Lebanese blood donors: significance and possible implications

Show simple item record El-Zaatari M. Kazma H. Naboulsi-Majzoub M. Haidar M. Ramlawi F. Mahfoud Z. Ramia S.
dc.contributor.editor 2007 2017-10-18T13:38:19Z 2017-10-18T13:38:19Z 2007
dc.identifier 10.1016/j.jhin.2007.04.010
dc.identifier.issn 01956701
dc.description.abstract Transmission of HBV infection through transfusion of HBsAg-negative blood has been documented. It is evident that low levels of HBV-DNA remain detectable in serum and liver tissue of some patients who clear HbsAg, and that the detection rate is highest in individuals who are 'anti-HBc positive alone'. This study was designed to assess the frequency and clinical significance of 'anti-HBc alone' in Lebanese blood donors. A total of 5511 blood donor samples from three major hospitals representing most regions of the country were tested for anti-HBc, amongst other screening tests. Samples positive for 'anti-HBc alone' were then tested for HBV-DNA and any positive for HBV-DNA were then genotyped and investigated for hepatitis B viral load. The study showed that 203 (3.7percent) of randomly selected Lebanese blood donors were confirmed as 'anti-HBc alone'. Of these, 11 (5.4percent) were HBV-DNA positive as detected by nested PCR. All samples had HBV-DNA levels below 400 copies-ml and all were genotype D. It can be concluded that HBV was present, although the circulating amount of virus was below the detectable limit for the assay used. Therefore, routine screening for anti-HBc may be required in Lebanese blood donation centres as an additional preventive measure for controlling transmission of HBV via blood transfusion. © 2007 The Hospital Infection Society.
dc.format.extent Pages: (278-282)
dc.language English
dc.publisher LONDON
dc.relation.ispartof Publication Name: Journal of Hospital Infection; Publication Year: 2007; Volume: 66; no. 3; Pages: (278-282);
dc.source Scopus
dc.title Hepatitis B virus DNA in serum of 'anti-HBc only'-positive healthy Lebanese blood donors: significance and possible implications
dc.type Article
dc.contributor.affiliation El-Zaatari, M., Department of Laboratory Medicine, Hammoud Hospital, Sidon, Lebanon, Medical Laboratory Technology, Faculty of Health Sciences, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Kazma, H., Department of Laboratory Medicine, Hammoud Hospital, Sidon, Lebanon
dc.contributor.affiliation Naboulsi-Majzoub, M., Islamic Hospital, Tripoli, Lebanon
dc.contributor.affiliation Haidar, M., Bahman Hospital, Beirut, Lebanon
dc.contributor.affiliation Ramlawi, F., Medical Laboratory Technology, Faculty of Health Sciences, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Mahfoud, Z., Department of Epidemiology and Population Health, Faculty of Health Sciences, American University of Beirut, Beirut, Lebanon
dc.contributor.affiliation Ramia, S., Medical Laboratory Technology, Faculty of Health Sciences, American University of Beirut, Beirut, Lebanon
dc.contributor.authorAddress Ramia, S.; Medical Laboratory Technology, Faculty of Health Sciences, American University of Beirut, Beirut, Lebanon; email:
dc.contributor.authorCorporate University: American University of Beirut; Faculty: Faculty of Health Sciences; Department: Medical Laboratory Technology;
dc.contributor.authorDepartment Medical Laboratory Technology
dc.contributor.faculty Faculty of Health Sciences
dc.contributor.authorInitials El-Zaatari, M
dc.contributor.authorInitials Kazma, H
dc.contributor.authorInitials Naboulsi-Majzoub, M
dc.contributor.authorInitials Haidar, M
dc.contributor.authorInitials Ramlawi, F
dc.contributor.authorInitials Mahfoud, Z
dc.contributor.authorInitials Ramia, S
dc.contributor.authorReprintAddress Ramia, S (reprint author), Amer Univ Beirut, Med Lab Technol, Fac Hlth Sci, Beirut, Lebanon.
dc.contributor.authorUniversity American University of Beirut
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dc.description.citedCount 12
dc.description.citedTotWOSCount 14
dc.description.citedWOSCount 11
dc.format.extentCount 5
dc.identifier.coden JHIND
dc.identifier.pubmedID 17540476
dc.identifier.scopusID 34250801505
dc.publisher.address 32 JAMESTOWN RD, LONDON NW1 7BY, ENGLAND
dc.relation.ispartOfISOAbbr J. Hosp. Infect.
dc.relation.ispartOfIssue 3
dc.relation.ispartofPubTitle Journal of Hospital Infection
dc.relation.ispartofPubTitleAbbr J. Hosp. Infect.
dc.relation.ispartOfVolume 66
dc.source.ID WOS:000248507400013
dc.type.publication Journal
dc.subject.otherAuthKeyword Antibody to Hepatitis B core antigen (anti-HBc)
dc.subject.otherAuthKeyword Blood donors
dc.subject.otherAuthKeyword Hepatitis B virus DNA
dc.subject.otherChemCAS DNA, Viral
dc.subject.otherChemCAS Hepatitis B Antibodies
dc.subject.otherChemCAS Hepatitis B Core Antigens
dc.subject.otherIndex hepatitis B core antibody
dc.subject.otherIndex virus DNA
dc.subject.otherIndex adult
dc.subject.otherIndex antibody detection
dc.subject.otherIndex article
dc.subject.otherIndex blood donor
dc.subject.otherIndex blood sampling
dc.subject.otherIndex DNA determination
dc.subject.otherIndex female
dc.subject.otherIndex genotype
dc.subject.otherIndex hepatitis B
dc.subject.otherIndex Hepatitis B virus
dc.subject.otherIndex human
dc.subject.otherIndex infection risk
dc.subject.otherIndex Lebanon
dc.subject.otherIndex major clinical study
dc.subject.otherIndex male
dc.subject.otherIndex nonhuman
dc.subject.otherIndex preventive health service
dc.subject.otherIndex serology
dc.subject.otherIndex virus detection
dc.subject.otherIndex virus identification
dc.subject.otherIndex virus load
dc.subject.otherIndex virus transmission
dc.subject.otherIndex Blood Donors
dc.subject.otherIndex Carrier State
dc.subject.otherIndex DNA, Viral
dc.subject.otherIndex Hepatitis B Antibodies
dc.subject.otherIndex Hepatitis B Core Antigens
dc.subject.otherIndex Hepatitis B virus
dc.subject.otherIndex Hepatitis B, Chronic
dc.subject.otherIndex Humans
dc.subject.otherIndex Lebanon
dc.subject.otherIndex Mass Screening
dc.subject.otherIndex Polymerase Chain Reaction
dc.subject.otherIndex Seroepidemiologic Studies
dc.subject.otherKeywordPlus CORE ANTIGEN
dc.subject.otherKeywordPlus SURFACE-ANTIGEN
dc.subject.otherKeywordPlus GENOTYPE-D
dc.subject.otherKeywordPlus INFECTION
dc.subject.otherKeywordPlus OCCULT
dc.subject.otherKeywordPlus INDIVIDUALS
dc.subject.otherKeywordPlus FREQUENCY
dc.subject.otherKeywordPlus FRAGMENT
dc.subject.otherKeywordPlus MARKERS
dc.subject.otherKeywordPlus HBSAG
dc.subject.otherWOS Infectious Diseases

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