7-O-methylpunctatin, a novel homoisoflavonoid, inhibits phenotypic switch of human arteriolar smooth muscle cells
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MDPI AG
Abstract
Remodeling of arterioles is a pivotal event in the manifestation of many inflammation-based cardio-vasculopathologies, such as hypertension. During these remodeling events, vascular smooth muscle cells (VSMCs) switch from a contractile to a synthetic phenotype. The latter is characterized by increased proliferation, migration, and invasion. Compounds with anti-inflammatory actions have been successful in attenuating this phenotypic switch. While the vast majority of studies investigating phenotypic modulation were undertaken in VSMCs isolated from large vessels, little is known about the effect of such compounds on phenotypic switch in VSMCs of microvessels (microVSMCs). We have recently characterized a novel homoisoflavonoid that we called 7-O-methylpunctatin (MP). In this study, we show that MP decreased FBS-induced cell proliferation, migration, invasion, and adhesion. MP also attenuated adhesion of THP-1 monocytes to microVSMCs, abolished FBS-induced expression of MMP-2, MMP-9, and NF-κB, as well as reduced activation of ERK1/2 and FAK. Furthermore, MP-treated VSMCs showed an increase in early (myocardin, SM-22α, SM-α) and mid-term (calponin and caldesmon) differentiation markers and a decrease in osteopontin, a protein highly expressed in synthetic VSMCs. MP also reduced transcription of cyclin D1, CDK4 but increased protein levels of p21 and p27. Taken together, these results corroborate an anti-inflammatory action of MP on human microVSMCs. Therefore, by inhibiting the synthetic phenotype of microVSMCs, MP may be a promising modulator for inflammation-induced arteriolar pathophysiology. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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7-o-methylpunctatin, Arterioles, Homoisoflavonoids, Inflammation, Phenotypic switch, Vascular smooth muscle cells, Cell movement, Cell proliferation, Cells, cultured, Gene expression regulation, developmental, Humans, Isoflavones, Matrix metalloproteinase 2, Matrix metalloproteinase 9, Myocytes, smooth muscle, Nf-kappa b, Phenotype, Signal transduction, 7 o methylpunctatin, Caldesmon, Calponin, Cyclin d1, Cyclin dependent kinase 4, Gelatinase a, Gelatinase b, Immunoglobulin enhancer binding protein, Isoflavonoid, Mitogen activated protein kinase 1, Mitogen activated protein kinase 3, Myocardin, Osteopontin, Protein p21, Protein p27, Unclassified drug, Isoflavone derivative, Antiinflammatory activity, Arterial smooth muscle, Article, Brdu assay, Cell adhesion, Cell adhesion assay, Cell culture technique, Cell cycle assay, Cell invasion, Cell invasion assay, Cell migration, Cell viability, Controlled study, Dna synthesis, Down regulation, Flow cytometry, Gene amplification, Gene expression, Gene sequence, Heart ventricle remodeling, Human, Human cell, Hypertension, Luciferase assay, Mtt assay, Phase contrast microscopy, Phenotypic variation, Protein expression, Protein expression level, Real time polymerase chain reaction, Scanning electron microscopy, Upregulation, Vascular smooth muscle cell, Western blotting, Wound healing assay, Arteriole, Cell culture, Cell motion, Drug effect, Gene expression regulation, Genetics, Metabolism, Smooth muscle cell