Lack of monoacylglycerol lipase prevents hepatic steatosis by favoring lipid storage in adipose tissue and intestinal malabsorption
Loading...
Files
Date
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Biochemistry and Molecular Biology Inc.
Abstract
Monoacylglycerol lipase (MGL) is the rate-limiting enzyme in the degradation of monoacylglycerols. To examine the role of MGL in hepatic steatosis, WT and MGL KO (MGL/) mice were challenged with a Western diet (WD) over 12 weeks. Lipid metabolism, inflammation, and fibrosis were assessed by serum biochemistry, histology, and gene-expression profiling of liver and adipose depots. Intestinal fat absorption was measured by gas chromatography. Primary adipocyte and 3T3-L1 cells were analyzed by flow cytometry and Western blot. Human hepatocytes were treated with MGL inhibitor JZL184. The absence of MGL protected mice from hepatic steatosis by repressing key lipogenic enzymes in liver (Srebp1c, Ppar2, and diacylglycerol O-acyltransferase 1), while promoting FA oxidation. Liver inflammation was diminished in MGL/mice fed a WD, as evidenced by diminished epidermal growth factor-like module-containing mucin-like hormone receptor-like 1 (F4/80) staining and C-C motif chemokine ligand 2 gene expression, whereas fibrosis remained unchanged. Absence of MGL promoted fat storage in gonadal white adipose tissue (gWAT) with increased lipogenesis and unchanged lipolysis, diminished inflammation in gWAT, and subcutaneous AT. Intestinal fat malabsorption prevented ectopic lipid accumulation in livers of MGL/mice fed a WD. In vitro experiments demonstrated increased adipocyte size/lipid content driven by PPAR. In conclusion, our data uncover that MGL deletion improves some aspects of nonalcoholic fatty liver disease by promoting lipid storage in gWAT and fat malabsorption.—Tardelli, M., F. V. Bruschi, T. Claudel, C. D. Fuchs, N. Auer, V. Kunczer, T. Stojakovic, H. Scharnagl, A. Habib, G. F. Grabner, R. Zimmermann, S. Lotersztajn, and M. Trauner. Lack of monoacylglycerol lipase prevents hepatic steatosis by favoring lipid storage in adipose tissue and intestinal malabsorption. J. Lipid Res. 2019. 60: 1284–1292. Copyright © 2019 Tardelli et al.
Description
Keywords
Adipocyte, Adipocytes/obesity, Fatty acid/metabolism, Lipolysis and fatty acid metabolism, Monoacylglycerol lipase, Nonalcoholic fatty liver disease, Nuclear receptors/peroxisome proliferator-activated receptor, Obesity, 3-hydroxybutyric acid, 3t3-l1 cells, Adiponectin, Adipose tissue, Animals, Blotting, western, Cells, cultured, Fatty acids, Glycerol, Humans, Immunohistochemistry, Insulin, Intestinal absorption, Lipid metabolism, Lipolysis, Liver, Mice, Mice, inbred c57bl, Monoacylglycerol lipases, Oxidation-reduction, Peroxisome proliferator-activated receptors, Triglycerides, 4 [bis(1,3 benzodioxol 5 yl)hydroxymethyl] 1 piperidinecarboxylic acid 4 nitrophenyl ester, Acylglycerol lipase, Diacylglycerol acyltransferase 1, Fatty acid, Lipid, Monocyte chemotactic protein 1, Peroxisome proliferator activated receptor gamma 2, Sterol regulatory element binding protein 1c, 3 hydroxybutyric acid, Peroxisome proliferator activated receptor, Triacylglycerol, 3t3-l1 cell line, Animal cell, Animal experiment, Animal model, Animal tissue, Article, Blood biochemistry, Controlled study, Enzyme activity, Enzyme analysis, Enzyme inhibition, Fatty acid oxidation, Fatty liver, Flow cytometry, Gas chromatography, Gene deletion, Gene expression, Gene expression profiling, Hepatitis, Histopathology, Inflammation, Knockout mouse, Lipid absorption, Lipid storage, Lipogenesis, Liver fibrosis, Malabsorption, Male, Mouse, Nonhuman, Priority journal, Protection, Subcutaneous fat, Western blotting, Western diet, White adipose tissue, Wild type mouse, Animal, Blood, C57bl mouse, Cell culture, Enzymology, Genetics, Human, Intestine absorption, Metabolism, Oxidation reduction reaction, Physiology