StarD13 differentially regulates migration and invasion in prostate cancer cells
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Springer Japan
Abstract
Prostate cancer is the second most commonly diagnosed cancer in men and one of the main leading causes of cancer deaths among men worldwide. Rapid uncontrolled growth and the ability to metastasize to other sites are key hallmarks in cancer development and progression. The Rho family of GTPases and its activators the GTPase-activating proteins (GAPs) are required for regulating cancer cell proliferation and migration. StarD13 is a GAP for Rho GTPases, specifically for RhoA and Cdc42. We have previously shown that StarD13 acts as a tumor suppressor in astrocytoma as well as breast and colorectal cancer. In this study, we performed a functional comparative analysis of StarD13 targets/and or interacting molecules to understand the general role that StarD13 plays in cancers. Our data highlight the importance of StarD13 in modulating several hallmarks of cancer. Findings from database mining and immunohistochemistry revealed that StarD13 is underexpressed in prostate cancers, in addition knocking down Stard13 increased cancer cell proliferation, consistent with its role as a tumor suppressor. Stard13 depletion, however, led to an increase in cell adhesion, which inhibited 2D cell migration. Most interestingly, StarD13 depletion increases invasion and matrix degradation, at least in part, through its regulation of Cdc42. Altogether, the data presented suggest that StarD13 acts as a tumor suppressor inhibiting prostate cancer cell invasion. © 2021, Japan Human Cell Society.
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Cell motility, Metastasis, Prostate cancer, Rho gap, Rho gtpases, Stard13, Cdc42 gtp-binding protein, Cell line, tumor, Cell movement, Disease progression, Gene expression, Gtpase-activating proteins, Humans, Male, Neoplasm invasiveness, Prostatic neoplasms, Rho gtp-binding proteins, Rhoa gtp-binding protein, Tumor suppressor proteins, Androgen, Beta catenin, Cdh2 protein, Cdh5 protein, Connective tissue growth factor, Cycline, Erk protein, Fkbp51 protein, Gelatin, Gelatinase a, Gelatinase b, Hoxb4 protein, Paxillin, Protein, Protein cdc42, Rac1 protein, Rho guanine nucleotide binding protein, Rhoa guanine nucleotide binding protein, Rock protein, Small interfering rna, Stard13 protein, Taz protein, Tp53inp1 protein, Transcription factor snail, Transcription factor sox2, Transcription factor twist, Unclassified drug, Uvomorulin, Vimentin, Cdc42 protein, human, Guanosine triphosphatase activating protein, Rho gtpase-activating protein, Rhoa protein, human, Stard13 protein, human, Tumor suppressor protein, Article, Cell adhesion, Cell invasion, Cell migration, Cell viability, Comparative study, Controlled study, Du145 cell line, Epithelial mesenchymal transition, Genetic transfection, Immunohistochemistry, In vitro study, Microarray analysis, Migration, Mrna expression level, Priority journal, Protein depletion, Protein expression, Protein protein interaction, Signal transduction, Western blotting, Wound healing assay, Wst-1 assay, Cell motion, Disease exacerbation, Genetics, Human, Metabolism, Pathology, Physiology, Prostate tumor, Tumor cell line, Tumor invasion