A novel HPLC-DAD method for simultaneous determination of febuxostat and diclofenac in biological samples: Pharmacokinetic outcomes
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Future Medicine Ltd.
Abstract
Aim: To develop a simple HPLC-DAD method for simultaneous determination of febuxostat (FEB) and diclofenac (DIC) in biological samples to assess pharmacokinetic outcomes of their coadministration. Methodology & results: Sample preparation was performed by liquid-liquid extraction. Drugs analysis was done on C18 column using methanol-formic acid pH 2.1 (76:24, v/v) as mobile phase and time-programmed UV detection. Lower limits of quantitation for FEB and DIC were 10 and 20 ng/ml, respectively. Baseline pharmacokinetics were similar to published data on either drug alone. Coadministration led to more than twofold increase in FEB C max and AUC together with a reduced hepatic uptake in rats. Conclusion: DIC interfered with initial distribution and terminal clearance of FEB potentially due to reduced FEB hepatic uptake. © 2018 Newlands Press.
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Keywords
Diclofenac, Febuxostat, Hplc-dad, Human plasma, Liver uptake, Pharmacokinetics, Adult, Animals, Area under curve, Calibration, Chromatography, high pressure liquid, Cross-over studies, Healthy volunteers, Humans, Liquid-liquid extraction, Liver, Male, Rats, Rats, sprague-dawley, Reproducibility of results, Sensitivity and specificity, Tissue distribution, Area under the curve, Article, Diode array detector, Drug clearance, Drug determination, Drug distribution, Drug uptake, High performance liquid chromatography, Human, Human experiment, Limit of detection, Limit of quantitation, Liquid liquid extraction, Maximum concentration, Nonhuman, Normal human, Rat, Animal, Blood, Crossover procedure, Metabolism, Reproducibility, Sprague dawley rat