Involvement of T-type calcium channels in the mechanism of low dose morphine-induced hyperalgesia in adult male rats
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Churchill Livingstone
Abstract
It has been shown that systemic and local administration of ultra-low dose morphine induced a hyperalgesic response via mu-opioid receptors. However, its exact mechanism(s) has not fully been clarified. It is documented that mu-opioid receptors functionally couple to T-type voltage dependent Ca+2 channels. Here, we investigated the role of T-type calcium channels, amiloride and mibefradil, on the induction of low-dose morphine hyperalgesia in male Wistar rats. The data showed that morphine (0.01 μg i.t. and 1 μg/kg i.p.) could elicit hyperalgesia as assessed by the tail-flick test. Administration of amiloride (5 and 10 μg i.t.) and mibefradil (2.5 and 5 μg i.t.) completely blocked low-dose morphine-induced hyperalgesia in spinal dorsal horn. Amiloride at doses of 1 and 5 mg/kg (i.p.) and mibefradil (9 mg/kg ip) 10 min before morphine (1 μg/kg i.p.) inhibited morphine-induced hyperalgesia. Our results indicate a role for T-type calcium channels in low dose morphine-induced hyperalgesia in rats. © 2021 Elsevier Ltd
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Amiloride, Hyperalgesia, Mibefradil, Morphine, Rat, T-type calcium channel, Analgesics, opioid, Animals, Calcium channels, t-type, Dose-response relationship, drug, Injections, intraperitoneal, Male, Pain measurement, Pain threshold, Posterior horn cells, Rats, Rats, wistar, Receptors, opioid, mu, Calcium channel t type, Mu opiate receptor, Narcotic analgesic agent, Adult, Animal experiment, Animal model, Article, Clinical article, Controlled study, Drug delivery system, Low drug dose, Nociception, Nonhuman, Spinal cord dorsal horn, Tail flick test, Wistar rat, Animal, Dose response, Drug effect, Intraperitoneal drug administration, Posterior horn cell