Drosophila tet is expressed in midline glia and is required for proper axonal development
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Frontiers Media S.A.
Abstract
Ten-Eleven Translocation (TET) proteins are important epigenetic regulators that play a key role in development and are frequently deregulated in cancer. Drosophila melanogaster has a single homologous Tet gene (dTet) that is highly expressed in the central nervous system during development. Here, we examined the expression pattern of dTet in the third instar larval CNS and discovered its presence in a specific set of glia cells: midline glia (MG). Moreover, dTet knockdown resulted in significant lethality, locomotor dysfunction, and alterations in axon patterning in the larval ventral nerve cord. Molecular analyses on dTet knockdown larvae showed a downregulation in genes involved in axon guidance and reduced expression of the axon guidance cue Slit. Our findings point toward a potential role for dTet in midline glial function, specifically the regulation of axon patterning during neurodevelopment. © 2019 Ismail, Badini, Frey, Abou-Kheir and Shirinian.
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Axon guidance cue, Axon guidance defect, Drosophila, Midline glia, Neurodevelopment, Tet, Beta actin, Drosophila protein, Ten eleven translocation protein, Unclassified drug, Article, Axon, Axon guidance, Central nervous system, Confocal laser scanning microscopy, Controlled study, Developmental delay, Dna extraction, Dot hybridization, Down regulation, Drosophila melanogaster, Epigenetics, Gene, Gene expression, Gene knockdown, Gene overexpression, Genotype, Glia cell, Immunofluorescence, Larval development, Lethality, Locomotion, Nerve cell differentiation, Nonhuman, Protein expression, Real time polymerase chain reaction, Rna extraction, Upregulation, Ventral root, Western blotting