Fluorescent-Based Thermal Sensing in Lipid Membranes
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American Chemical Society
Abstract
Thermal mapping in biological membranes could unlock and help us understand many chemical and physical processes that do not only pertain to localized membrane phenomena but also extend to many other intra- and extracellular pathways. In this manuscript, we report the development of a ratiometric thermal fluorescent probe based on the Förster resonance energy transfer between a lipid-embedded conjugated polyelectrolyte and a lyophilic acceptor dye. We showed that the Förster resonance energy transfer (FRET) pair is sensitive within the relevant physiological temperature window (20.0-50.0 °C). The signal was also shielded from an external pH and stable when cycled multiple times. The probe was also sensitive to the membrane composition and could, therefore, be further developed to probe the membrane composition and viscosity. Copyright © 2020 American Chemical Society.
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1,2-dipalmitoylphosphatidylcholine, Carbocyanines, Fluorescence, Fluorescence resonance energy transfer, Fluorescent dyes, Hydrogen-ion concentration, Liposomes, Polyelectrolytes, Polyvinyls, Temperature, Viscosity, Biological membranes, Cytology, Energy transfer, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine, Carbocyanine, Dipalmitoylphosphatidylcholine, Fluorescent dye, Liposome, Poly(2,5-methoxy-propyloxy sulfonate phenylene vinylene), Polyelectrolyte, Polyvinyl derivative, Conjugated polyelectrolytes, Fluorescent probes, Lipid membranes, Membrane composition, Physical process, Physiological temperature, Resonance energy transfer, Thermal sensing, Chemistry, Ph