Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions

Simple item page
dc.contributor.authorDelcourt, Vivian
dc.contributor.authorFranck, Julien
dc.contributor.authorQuanico, Jusal P.
dc.contributor.authorGimeno, Jean Pascal
dc.contributor.authorWisztorski, Maxence
dc.contributor.authorRaffo-Romero, Antonella
dc.contributor.authorKobeissy, Firas H.
dc.contributor.authorRoucou, Xavier
dc.contributor.authorSALZET, Michel
dc.contributor.authorFournier, Isabelle
dc.contributor.departmentBiochemistry and Molecular Genetics
dc.contributor.facultyFaculty of Medicine (FM)
dc.contributor.institutionAmerican University of Beirut
dc.date.accessioned2025-01-24T11:37:55Z
dc.date.available2025-01-24T11:37:55Z
dc.date.issued2018
dc.description.abstractTissue spatially-resolved proteomics was performed on 3 brain regions, leading to the characterization of 123 reference proteins. Moreover, 8 alternative proteins from alternative open reading frames (AltORF) were identified. Some proteins display specific post-translational modification profiles or truncation linked to the brain regions and their functions. Systems biology analysis performed on the proteome identified in each region allowed to associate sub-networks with the functional physiology of each brain region. Back correlation of the proteins identified by spatially-resolved proteomics at a given tissue localization with the MALDI MS imaging data, was then performed. As an example, mapping of the distribution of the matrix metallopeptidase 3-cleaved C-terminal fragment of -synuclein (aa 95–140) identified its specific distribution along the hippocampal dentate gyrus. Taken together, we established the molecular physiome of 3 rat brain regions through reference and hidden proteome characterization. Molecular & Cellular Proteomics 17: 10.1074/mcp.M116.065755, 357–372, 2018.
dc.identifier.doihttps://doi.org/10.1074/mcp.M116.065755
dc.identifier.eid2-s2.0-85041730557
dc.identifier.pmid29122912
dc.identifier.urihttp://hdl.handle.net/10938/28924
dc.language.isoen
dc.publisherAmerican Society for Biochemistry and Molecular Biology Inc.
dc.relation.ispartofMolecular and Cellular Proteomics
dc.sourceScopus
dc.subjectAnimals
dc.subjectBrain
dc.subjectMale
dc.subjectProteome
dc.subjectProteomics
dc.subjectRats, wistar
dc.subjectSpectrometry, mass, matrix-assisted laser desorption-ionization
dc.subjectAlpha synuclein
dc.subjectApex protein
dc.subjectBeta synuclein
dc.subjectCalmodulin
dc.subjectChromogranin b
dc.subjectCytochrome c oxidase
dc.subjectGamma synuclein
dc.subjectHematological and neurological expressed 1
dc.subjectLipid
dc.subjectMyelin basic protein
dc.subjectNeuromodulin
dc.subjectOxidoreductase
dc.subjectParathymosin
dc.subjectPep 19 protein
dc.subjectProenkephalin
dc.subjectProtein
dc.subjectSecretogranin ii
dc.subjectSomatostatin
dc.subjectStromelysin
dc.subjectSynaptosomal associated protein 25
dc.subjectThymosin beta10
dc.subjectThymosin beta4
dc.subjectUbiquitin
dc.subjectUnclassified drug
dc.subject3' untranslated region
dc.subject5' untranslated region
dc.subjectAnimal tissue
dc.subjectArticle
dc.subjectBrain function
dc.subjectBrain region
dc.subjectBrain tissue
dc.subjectCell damage
dc.subjectCell proliferation
dc.subjectControlled study
dc.subjectCorpus callosum
dc.subjectEnzyme activity
dc.subjectHippocampus
dc.subjectMatrix-assisted laser desorption-ionization mass spectrometry
dc.subjectMedulla oblongata
dc.subjectNervous system development
dc.subjectNonhuman
dc.subjectOxidative stress
dc.subjectPriority journal
dc.subjectProtein analysis
dc.subjectRat
dc.subjectSynaptogenesis
dc.subjectAnimal
dc.subjectMetabolism
dc.subjectWistar rat
dc.titleSpatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions
dc.typeArticle

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
2018-8458.pdf
Size:
4.29 MB
Format:
Adobe Portable Document Format
Download

Collections

Biochemistry and Molecular Genetics