Gene expression profiling of breast cancer in Lebanese women

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dc.contributor.authorMakoukji, Joelle
dc.contributor.authorMakhoul, Nadine J.
dc.contributor.authorKhalil, Maya K.
dc.contributor.authorEl-Sitt, Sally Boulos
dc.contributor.authorSaad Aldin, Ehab
dc.contributor.authorJabbour, Mark N.
dc.contributor.authorBoulos, Fouad I.
dc.contributor.authorGadaleta, Emanuela
dc.contributor.authorSangaralingam, Ajanthah
dc.contributor.authorChelala, Claude
dc.contributor.authorBoustany, Rose Mary Naaman
dc.contributor.authorTfayli, Arafat Hussein
dc.contributor.departmentBiochemistry and Molecular Genetics
dc.contributor.departmentPathology and Laboratory Medicine
dc.contributor.departmentPediatrics and Adolescent Medicine
dc.contributor.departmentInternal Medicine
dc.contributor.departmentDivision of Pediatric Neurology
dc.contributor.facultyFaculty of Medicine (FM)
dc.contributor.institutionAmerican University of Beirut
dc.date.accessioned2025-01-24T11:37:49Z
dc.date.available2025-01-24T11:37:49Z
dc.date.issued2016
dc.description.abstractBreast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2.0 arrays. Quantitative real-time PCR was employed to validate candidate genes. Differentially expressed genes between breast cancer and non-tumor tissues were screened. Significant differences in gene expression were established for COL11A1/COL10A1/MMP1/COL6A6/DLK1/S100P/CXCL11/SOX11/LEP/ADIPOQ/OXTR/FOSL1/ACSBG1 and C21orf37. Pathways/diseases representing these genes were retrieved and linked using PANTHER ®/Pathway Studio ®. Many of the deregulated genes are associated with extracellular matrix, inflammation, angiogenesis, metastasis, differentiation, cell proliferation and tumorigenesis. Characteristics of breast cancers in Lebanese were compared to those of women from Western populations to explain why breast cancer is more aggressive and presents a decade earlier in Lebanese victims. Delineating molecular mechanisms of breast cancer in Lebanese women led to key genes which could serve as potential biomarkers and/or novel drug targets for breast cancer. © 2016 The Author(s).
dc.identifier.doihttps://doi.org/10.1038/srep36639
dc.identifier.eid2-s2.0-84995890355
dc.identifier.pmid27857161
dc.identifier.urihttp://hdl.handle.net/10938/28884
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.sourceScopus
dc.subjectBreast neoplasms
dc.subjectFemale
dc.subjectGene expression profiling
dc.subjectHumans
dc.subjectLebanon
dc.subjectMiddle aged
dc.subjectOligonucleotide array sequence analysis
dc.subjectProtein interaction maps
dc.subjectReal-time polymerase chain reaction
dc.subjectSignal transduction
dc.subjectBreast tumor
dc.subjectDna microarray
dc.subjectEthnology
dc.subjectGenetics
dc.subjectHuman
dc.subjectMetabolism
dc.subjectPathology
dc.subjectProtein protein interaction
dc.subjectReal time polymerase chain reaction
dc.subjectValidation study
dc.titleGene expression profiling of breast cancer in Lebanese women
dc.typeArticle

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