Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site
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Academic Press Inc.
Abstract
The cleavage motif in the hemagglutinin (HA) protein of highly pathogenic H5 and H7 subtypes of avian influenza viruses is characterized by a peptide insertion or a multibasic cleavage site (MBCS). Here, we isolated an H4N2 virus from quails (Quail/CA12) with two additional arginines in the HA cleavage site, PEK. RRTR/G, forming an MBCS-like motif. Quail/CA12 is a reassortant virus with the HA and neuraminidase (NA) gene most similar to a duck-isolated H4N2 virus, PD/CA06 with a monobasic HA cleavage site. Quail/CA12 required exogenous trypsin for efficient growth in culture and caused no clinical illness in infected chickens. Quail/CA12 had high binding preference for α2,6-linked sialic acids and showed higher replication and transmission ability in chickens and quails than PD/CA06. Although the H4N2 virus remained low pathogenic, these data suggests that the acquisition of MBCS in the field is not restricted to H5 or H7 subtypes. © 2014 Elsevier Inc.
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H4n2, Low pathogenic avian influenza, Multibasic cleavage site, Outbreak, Quail, Amino acid sequence, Animals, Ferrets, Gene expression regulation, viral, Hemagglutinins, Influenza a virus, Influenza in birds, Molecular sequence data, Orthomyxoviridae infections, Phylogeny, Aves, Orthomyxoviridae, Phasianidae, Arginine, Influenza virus hemagglutinin, Sialic acid, Trypsin, Virus rna, Virus sialidase, Hemagglutinin, Animal cell, Animal experiment, Animal model, Article, Avian influenza, Avian influenza virus, Binding affinity, Chicken, Controlled study, Culture medium, Duck, Ferret, Genetic reassortment, Hemagglutinin gene, In vitro study, Influenza virus a h4n2, Neuraminidase gene, Nonhuman, Nucleotide sequence, Priority journal, Protein analysis, Protein cleavage, Protein motif, Sequence homology, Species comparison, Virogenesis, Virus characterization, Virus isolation, Virus replication, Virus transmission, Virus virulence, Animal tissue, Human, Human cell, Immunohistochemistry, Influenza, Influenza virus, Influenza virus h4n2, Pathogenicity, Rna extraction, Virus cell interaction, Virus infectivity, Animal, Chemistry, Classification, Gene expression regulation, Genetics, Metabolism, Molecular genetics, Orthomyxovirus infection, Transmission, Veterinary, Virology