Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site

Simple item page
dc.contributor.authorWong, Sooksan
dc.contributor.authorYoon, Sun-woo
dc.contributor.authorZanin, Mark P.
dc.contributor.authorSong, Min-suk
dc.contributor.authorOshansky, Christine M.
dc.contributor.authorZaraket, Hassan
dc.contributor.authorSonnberg, Stephanie
dc.contributor.authorRubrum, Adam M.
dc.contributor.authorSeiler, Patrick J.
dc.contributor.authorFerguson, Angela Damon
dc.contributor.authorKrauss, Scott L.
dc.contributor.authorCardona, Carol J.
dc.contributor.authorWebby, Richard John
dc.contributor.authorCrossley, Beate M.
dc.contributor.departmentExperimental Pathology, Microbiology, and Immunology
dc.contributor.facultyFaculty of Medicine (FM)
dc.contributor.institutionAmerican University of Beirut
dc.date.accessioned2025-01-24T11:38:53Z
dc.date.available2025-01-24T11:38:53Z
dc.date.issued2014
dc.description.abstractThe cleavage motif in the hemagglutinin (HA) protein of highly pathogenic H5 and H7 subtypes of avian influenza viruses is characterized by a peptide insertion or a multibasic cleavage site (MBCS). Here, we isolated an H4N2 virus from quails (Quail/CA12) with two additional arginines in the HA cleavage site, PEK. RRTR/G, forming an MBCS-like motif. Quail/CA12 is a reassortant virus with the HA and neuraminidase (NA) gene most similar to a duck-isolated H4N2 virus, PD/CA06 with a monobasic HA cleavage site. Quail/CA12 required exogenous trypsin for efficient growth in culture and caused no clinical illness in infected chickens. Quail/CA12 had high binding preference for α2,6-linked sialic acids and showed higher replication and transmission ability in chickens and quails than PD/CA06. Although the H4N2 virus remained low pathogenic, these data suggests that the acquisition of MBCS in the field is not restricted to H5 or H7 subtypes. © 2014 Elsevier Inc.
dc.identifier.doihttps://doi.org/10.1016/j.virol.2014.07.048
dc.identifier.eid2-s2.0-84906482809
dc.identifier.pmid25151061
dc.identifier.urihttp://hdl.handle.net/10938/29086
dc.language.isoen
dc.publisherAcademic Press Inc.
dc.relation.ispartofVirology
dc.sourceScopus
dc.subjectH4n2
dc.subjectLow pathogenic avian influenza
dc.subjectMultibasic cleavage site
dc.subjectOutbreak
dc.subjectQuail
dc.subjectAmino acid sequence
dc.subjectAnimals
dc.subjectFerrets
dc.subjectGene expression regulation, viral
dc.subjectHemagglutinins
dc.subjectInfluenza a virus
dc.subjectInfluenza in birds
dc.subjectMolecular sequence data
dc.subjectOrthomyxoviridae infections
dc.subjectPhylogeny
dc.subjectAves
dc.subjectOrthomyxoviridae
dc.subjectPhasianidae
dc.subjectArginine
dc.subjectInfluenza virus hemagglutinin
dc.subjectSialic acid
dc.subjectTrypsin
dc.subjectVirus rna
dc.subjectVirus sialidase
dc.subjectHemagglutinin
dc.subjectAnimal cell
dc.subjectAnimal experiment
dc.subjectAnimal model
dc.subjectArticle
dc.subjectAvian influenza
dc.subjectAvian influenza virus
dc.subjectBinding affinity
dc.subjectChicken
dc.subjectControlled study
dc.subjectCulture medium
dc.subjectDuck
dc.subjectFerret
dc.subjectGenetic reassortment
dc.subjectHemagglutinin gene
dc.subjectIn vitro study
dc.subjectInfluenza virus a h4n2
dc.subjectNeuraminidase gene
dc.subjectNonhuman
dc.subjectNucleotide sequence
dc.subjectPriority journal
dc.subjectProtein analysis
dc.subjectProtein cleavage
dc.subjectProtein motif
dc.subjectSequence homology
dc.subjectSpecies comparison
dc.subjectVirogenesis
dc.subjectVirus characterization
dc.subjectVirus isolation
dc.subjectVirus replication
dc.subjectVirus transmission
dc.subjectVirus virulence
dc.subjectAnimal tissue
dc.subjectHuman
dc.subjectHuman cell
dc.subjectImmunohistochemistry
dc.subjectInfluenza
dc.subjectInfluenza virus
dc.subjectInfluenza virus h4n2
dc.subjectPathogenicity
dc.subjectRna extraction
dc.subjectVirus cell interaction
dc.subjectVirus infectivity
dc.subjectAnimal
dc.subjectChemistry
dc.subjectClassification
dc.subjectGene expression regulation
dc.subjectGenetics
dc.subjectMetabolism
dc.subjectMolecular genetics
dc.subjectOrthomyxovirus infection
dc.subjectTransmission
dc.subjectVeterinary
dc.subjectVirology
dc.titleCharacterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site
dc.typeArticle

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
2014-10734.pdf
Size:
1.3 MB
Format:
Adobe Portable Document Format
Download

Collections

Experimental Pathology, Microbiology, and Immunology