Formation of liposome mediated poly (diallyldimethylammonium chloride) based nanoparticles towards biomedical application

Abstract

Nanosystems with various compositions and biological properties are being extensively investigated for drug and gene delivery applications. Many nanotechnology methods use novel nanocarriers, such as liposome, in therapeutic technology targeting drug delivery system. Liposomes are considered as extraordinary drug delivery system where they form vesicular structures consisting of bilayers, resulting from the dispersion of phospholipids in water. However, liposomes alone during drug delivery are known to suffer from drug leakage and instability. Therefore, the surface modification of the liposomes by coating or adding polymer has shown progress in their application in drug delivery. In this presented work, liposomes were synthesized according to the thin film hydration method. The drug efficiency of the liposome was established according to the release of curcumin from the liposomes. Further, we investigated the drug release efficiency of two different types of phospholipids liposomes; 1, 2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and mixture of 1, 2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and DMPC liposome. Three different nanocapsules were prepared using poly(diallyldimethylammonium)chloride (PDAA) polymer and silica nanoparticles, in order to assure complete encapsulation of curcumin into the core of the liposome. For liposomes, which were formed by mixture of DMPC and DPPC, we synthesized three different ratios of mixture. The prepared mixtures were modified by one layer of the cationic polymer (PDDA). Drug delivery profile was carried out for DMPC and mixture nanocapsules at three different pHs (4, 6 and 7) to test the release of curcumin. It was shown, at pH 4, curcumin exhibits higher release. Moreover, it was confirmed that when increasing the number of layers on the nanocapsule surface, curcumin exhibits lower curcumin release during 24 hours. Florescence, TGA, DLS, XRD, SEM, Zeta potential, encapsulation efficiency and drug loading were investigated for the different DMPC nanocapsules.