Chemical characterization and in vitro biological evaluation of aqueous extract of Althaea officinalis L. flower grown in Lebanon
Loading...
Files
Date
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier GmbH
Abstract
Introduction: Herbal medicine is extensively used in various therapeutic strategies. Althaea officinalis L. of the family Malvaceae exhibits many biological activities through its phenolic acids, flavonoids, coumarins and polysaccharides. In Lebanon, the flowers are consumed in hot infusions. Therefore, the current study aimed to evaluate chemical and biological properties of Lebanese Althaea officinalis L. flower water extract. Methods: Chemical characterization was performed by qualitative phytochemical screening, gas chromatography-mass spectrometry (GC/MS) and liquid chromatography-mass spectrometry (LC-MS). Anti-proliferative effect was evaluated in cancer cells by MTT assay. The mRNA expression levels of pro-inflammatory enzyme and inflammatory cytokines were assessed in LPS-stimulated RAW 264.7 cells. Cytoprotective activity was examined in red blood cells against hydrogen peroxide-induced hemolysis. Antioxidant property was assessed by DPPH radical scavenging assay. Results: Qualitative phytochemical screening of the extract revealed the presence of anthraquinones, flavonoids, lignins, phenols, tannins, terpenoids and others. GC/MS analysis suggested that most of the phytocompounds are conjugated to sugars rather than free. LC/MS analysis identified 5 phenolic acids (syringic, gallic, caffeic, p-coumaric and trans-ferulic acids) and 8 flavonoids (catechin, apigenin, chrysin, quercetin, kaempferol, genistein, rutin trihydrate and galangin). Biological evaluation of the extract showed anti-proliferative effects on A549, EB, HCT-116, MCF-7 and HeLa 229 cells, anti-inflammatory activity illustrated by decrease in mRNA expression of inducible nitric oxide synthase, interleukin 1 beta, tumor necrosis factor alpha and interleukin 6, cytoprotective activity in red blood cells and antioxidant property. Conclusion: Althaea officinalis flowers rich in phytocompounds exert interesting biological activities and hold promise in the pharmacological field. © 2022 Elsevier GmbH
Description
Keywords
Althaea officinalis, Anti-hemolytic, Anti-inflammatory, Antioxidant, Chemical analysis, Cytotoxicity, 2 o glycerol alpha galactopyranoside hexa trimethylsilyl, Alpha glucopyranose 1,2,3,4,6 pentakis o trimethylsilyl, Alpha glucopyranoside 1,3,4,6 tetrakis o trimethylsilyl beta fructofuranosyl 2,3,4,6 tetrakis o trimethylsilyl, Althaea officinalis extract, Amino acid derivative, Anthraquinone derivative, Antiinflammatory agent, Antineoplastic agent, Apigenin, Arabinitol pentakis o trimethylsilyl, Arabinose 2,3,4,5 tetrakis o trimethylsilyl, Beta glucopyranose 1,2,3,4,6 pentakis o trimethylsilyl, Caffeic acid, Carbohydrate derivative, Cardiac glycoside, Catechin, Chrysin, Cytoprotective agent, Fatty acid derivative, Flavanone derivative, Flavonoid, Fructose 1,3,4,5,6 pentakis o trimethylsilyl, Galactose 2,3,4,5,6 pentakis o trimethylsilyl, Galangin, Gallic acid, Genistein, Glucose 2,3,4,5,6 pentakis o trimethylsilyl, Hydrogen peroxide, Hydroxyacid, Inducible nitric oxide synthase, Interleukin 1beta, Interleukin 6, Kaempferol, Lignin, Lipopolysaccharide, Mannopyranose 1,2,3,4,6 pentakis o trimethylsilyl, Messenger rna, Myoinositol 1,2,3,4,5,6 hexakis o trimethylsilyl, Para coumaric acid, Phenol derivative, Phlobatannin, Phytochemical, Phytosterol, Plant extract, Plant protein, Quercetin, Quinone derivative, Resin, Ribitol 1,2,3,4,5 pentakis o trimethylsilyl, Ribose 2,3,4,5 tetrakis o trimethylsilyl o methyloxime, Rutin trihydrate, Syringic acid, Tannin derivative, Terpenoid derivative, Threitol 1,2,3,4 tetrakis o trimethylsilyl, Trans ferulic acid, Tumor necrosis factor, Unclassified drug, Vegetable oil, Water, Xylitol 2,3,4,5 tetrakis o trimethylsilyl, A-549 cell line, Althaea, Animal cell, Antiinflammatory activity, Antioxidant activity, Antiproliferative activity, Aqueous solution, Article, Biological activity, Cell protection, Colon cancer cell line, Conjugation, Controlled study, Dpph radical scavenging assay, Drug efficacy, Drug identification, Drug mechanism, Drug screening, Eb cell line, Erythrocyte, Ethnopharmacology, Female, Flower, Hct 116 cell line, Hela 229 cell line, Hemolysis, Human, Human cell, Ic50, In vitro study, Lebanon, Lipopolysaccharide-induced inflammation, Liquid chromatography-mass spectrometry, Mass fragmentography, Mcf-7 cell line, Mouse, Mrna expression level, Mtt assay, Nonhuman, Normal human, Phytochemistry, Qualitative analysis, Raw 264.7 cell line