Curcumin-graphene quantum dots for dual mode sensing platform: Electrochemical and fluorescence detection of APOe4, responsible of Alzheimer's disease
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Elsevier B.V.
Abstract
New dual electrochemical and fluorescence sensitive curcumin-graphene quantum dots sensing platform coated on the transparent Indium-Tin-Oxide electrode was developed to sense APOe4 DNA, responsible of Alzheimer's disease. Curcumin molecule with its dual fluorescence and electrochemical properties was electropolymerized on GQDs-ITO surface. EDC/NHS chemistry was used to covalently immobilize an amino-substituted DNA probe via a malonic acid spacer. Quenching of curcumin signals following hybridized DNA complex was employed to quantify APOe4 DNA. Amperometric studies revealed an ultrasensitive behavior toward the formation of DNA complex with a sensitivity of 4.74 nA.mL.pg −1 and a limit of detection as low as 0.48 pg mL −1. The platform exhibits very good performances such as repeatability, reproducibility, selectivity and long storage stability. Fluorescence results were established for the support and the complementarity of electrochemical results. Founded results confirmed the ultrasensitivity of platform with comparable performances. Recorded results in human blood plasma demonstrated the high efficacy of curcumin system sensing even in the clinical matrix. © 2018 Elsevier B.V.
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Alzheimer disease, Apoe4 allele, Curcumin, Dual fluorescence and electrochemical sensing, Graphene quantum dots, Ito transparent electrode, Apolipoprotein e4, Electrochemical techniques, Fluorescence, Graphite, Humans, Particle size, Quantum dots, Spectrometry, fluorescence, Chemical detection, Dna, Graphene, Indium compounds, Nanocrystals, Neurodegenerative diseases, Semiconductor quantum dots, Tin oxides, Transparent electrodes, 1 (3 dimethylaminopropyl) 3 ethylcarbodiimide, Curcumin graphene quantum dot, Indium, Malonic acid, N hydroxysuccinimide, Oxide, Quantum dot, Tin, Unclassified drug, Alzheimer's disease, Electrochemical sensing, Fluorescence detection, Human blood plasmas, Indium tin oxide electrodes, Amino acid substitution, Amperometry, Article, Controlled study, Dna probe, Electrochemical detection, Fluorescence analysis, Human, Hybridization, Limit of detection, Measurement repeatability, Photochemical quenching, Priority journal, Protein analysis, Reproducibility, Storage, Chemistry, Electrochemical analysis, Spectrofluorometry, Electrochemical electrodes