Curcumin-graphene quantum dots for dual mode sensing platform: Electrochemical and fluorescence detection of APOe4, responsible of Alzheimer's disease

Simple item page
dc.contributor.authorMars, Abdelmoneim
dc.contributor.authorHamami, Maroua
dc.contributor.authorBechnak, Linda
dc.contributor.authorPatra, Digambara
dc.contributor.authorRaouafi, Noureddine
dc.contributor.departmentDepartment of Chemistry
dc.contributor.facultyFaculty of Arts and Sciences (FAS)
dc.contributor.institutionAmerican University of Beirut
dc.date.accessioned2025-01-24T11:21:59Z
dc.date.available2025-01-24T11:21:59Z
dc.date.issued2018
dc.description.abstractNew dual electrochemical and fluorescence sensitive curcumin-graphene quantum dots sensing platform coated on the transparent Indium-Tin-Oxide electrode was developed to sense APOe4 DNA, responsible of Alzheimer's disease. Curcumin molecule with its dual fluorescence and electrochemical properties was electropolymerized on GQDs-ITO surface. EDC/NHS chemistry was used to covalently immobilize an amino-substituted DNA probe via a malonic acid spacer. Quenching of curcumin signals following hybridized DNA complex was employed to quantify APOe4 DNA. Amperometric studies revealed an ultrasensitive behavior toward the formation of DNA complex with a sensitivity of 4.74 nA.mL.pg −1 and a limit of detection as low as 0.48 pg mL −1. The platform exhibits very good performances such as repeatability, reproducibility, selectivity and long storage stability. Fluorescence results were established for the support and the complementarity of electrochemical results. Founded results confirmed the ultrasensitivity of platform with comparable performances. Recorded results in human blood plasma demonstrated the high efficacy of curcumin system sensing even in the clinical matrix. © 2018 Elsevier B.V.
dc.identifier.doihttps://doi.org/10.1016/j.aca.2018.06.075
dc.identifier.eid2-s2.0-85049737668
dc.identifier.pmid30253824
dc.identifier.urihttp://hdl.handle.net/10938/25393
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofAnalytica Chimica Acta
dc.sourceScopus
dc.subjectAlzheimer disease
dc.subjectApoe4 allele
dc.subjectCurcumin
dc.subjectDual fluorescence and electrochemical sensing
dc.subjectGraphene quantum dots
dc.subjectIto transparent electrode
dc.subjectApolipoprotein e4
dc.subjectElectrochemical techniques
dc.subjectFluorescence
dc.subjectGraphite
dc.subjectHumans
dc.subjectParticle size
dc.subjectQuantum dots
dc.subjectSpectrometry, fluorescence
dc.subjectChemical detection
dc.subjectDna
dc.subjectGraphene
dc.subjectIndium compounds
dc.subjectNanocrystals
dc.subjectNeurodegenerative diseases
dc.subjectSemiconductor quantum dots
dc.subjectTin oxides
dc.subjectTransparent electrodes
dc.subject1 (3 dimethylaminopropyl) 3 ethylcarbodiimide
dc.subjectCurcumin graphene quantum dot
dc.subjectIndium
dc.subjectMalonic acid
dc.subjectN hydroxysuccinimide
dc.subjectOxide
dc.subjectQuantum dot
dc.subjectTin
dc.subjectUnclassified drug
dc.subjectAlzheimer's disease
dc.subjectElectrochemical sensing
dc.subjectFluorescence detection
dc.subjectHuman blood plasmas
dc.subjectIndium tin oxide electrodes
dc.subjectAmino acid substitution
dc.subjectAmperometry
dc.subjectArticle
dc.subjectControlled study
dc.subjectDna probe
dc.subjectElectrochemical detection
dc.subjectFluorescence analysis
dc.subjectHuman
dc.subjectHybridization
dc.subjectLimit of detection
dc.subjectMeasurement repeatability
dc.subjectPhotochemical quenching
dc.subjectPriority journal
dc.subjectProtein analysis
dc.subjectReproducibility
dc.subjectStorage
dc.subjectChemistry
dc.subjectElectrochemical analysis
dc.subjectSpectrofluorometry
dc.subjectElectrochemical electrodes
dc.titleCurcumin-graphene quantum dots for dual mode sensing platform: Electrochemical and fluorescence detection of APOe4, responsible of Alzheimer's disease
dc.typeArticle

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
2018-7882.pdf
Size:
1.21 MB
Format:
Adobe Portable Document Format
Download

Collections

Department of Chemistry