dc.contributor.author |
Hamdar, Layal Hasan |
dc.date.accessioned |
2017-08-30T14:12:40Z |
dc.date.available |
2017-08-30T14:12:40Z |
dc.date.issued |
2015 |
dc.date.submitted |
2015 |
dc.identifier.other |
b18356242 |
dc.identifier.uri |
http://hdl.handle.net/10938/10853 |
dc.description |
Thesis. M.Sc. American University of Beirut. Department of Anatomy, Cell Biology, and Physiology 2015. W 4 H211es 2015 |
dc.description |
Advisor: Dr. Wassim Abou-Kheir, Assistant Professor, Department of Anatomy, Cell Biology, and Physiology ; Co-advisor, Dr. Assaad Eid, Assistant Professor, Department of Anatomy, Cell Biology, and Physiology ; Committee members: Dr. Georges Daoud, Assistant Professor, Department of Anatomy, Cell Biology, and Physiology ; Dr. Rihab Nasr, Associate Professor, Department of Anatomy, Cell Biology, and Physiology. |
dc.description |
Includes bibliographical references (leaves 41-44) |
dc.description |
Includes bibliographical references (leaves 41-44) |
dc.description.abstract |
Cell lines representing the progression of prostate cancer from an androgen-dependent to an androgen-independent state are scarce. Previously, we have established and characterized a new murine prostate luminal epithelial cell line (PLum), with Pten-TP53 deletions, derived from a prostate epithelial stem-progenitor-enriched cell population. The deprivation of androgens from established PLum-orthotopic tumors resulted in tumor regression and eventually castration-resistant growth. Cells derived from orthotopic tumors have been isolated to develop androgen-dependent versus androgen-independent model. In this study, several experiments were conducted to establish and investigate the functional differences of the newly isolated androgen-dependent (PLum- AD) and androgen-independent (PLum-AI) murine prostate cancer cell lines. Unlike PLum-AD cells that grew in serum-free medium, PLum-AI cells grew better in 5percent FBS-containing medium. Both cell lines remained faithful in morphology to their in vivo source, where PLum-AD showed a typical epithelial morphology (in vivo source: adenocarcinoma) and PLum-AI showed an epithelial-to-mesenchymal morphology (in vivo source: sarcomatoid carcinoma). Furthermore, upon immunofluorescent analysis, PLum-AD cell expressed mostly prostate epithelial markers while PLum-AI cells expressed mesenchymal cell markers. In addition, QRT-PCR and Western blot analysis confirmed the epithelial and mesenchymal morphology of PLum-AD and PLum-AI respectively. To assess for the presence of stem-progenitor cell population, the cells were subjected to sphere-formation assay. Both cell lines had the capacity to form spheres, where PLum-AD cells formed regular-shaped spheres and PLum-AI cells formed mostly large stellate shaped spheres consistent with their mesenchymal-like nature. Interestingly, and despite a lower level in PLum-AI, both cell lines showed expression of AR at the mRNA and protein levels. Although both cell lines showed tumorigenic abilities, PLum-AI was significantly more aggressive in t |
dc.format.extent |
1 online resource ( 44 leaves) |
dc.language.iso |
eng |
dc.relation.ispartof |
Theses, Dissertations, and Projects |
dc.subject.classification |
W 4 H211es 2015 |
dc.subject.lcsh |
Dissertations, Academic. |
dc.subject.lcsh |
Prostate. |
dc.subject.lcsh |
Urologic Diseases. |
dc.subject.lcsh |
Neoplasm. |
dc.title |
Establishing a novel in vitro model of prostate cancer starting from stem-progenitor cells - |
dc.type |
Thesis |
dc.contributor.department |
Department of Anatomy, Cell Biology and Physiological Sciences |
dc.contributor.faculty |
Faculty of Medicine |
dc.contributor.institution |
American University of Beirut |