Enhancement of physical and mechanical properties of Gel-MA towards application in wound healing procedures -

Abstract

Gelatin methacryloyl (GelMA) is a hydrogel that has found extensive use in the field of biomedicine, especially tissue engineering. GelMA hydrogels’ structure closely resemble the structure of the extra-cellular matrix (ECM) found in tissues and organs. Also, the fabrication process of GelMA enables the customization of its mechanical properties as seen fit for its use. This study investigates the potential of GelMA for application in regenerative medicine. The study will concentrate on the potential for use as a wound healing patch for patients suffering from chronic wounds. Various GelMA hydrogel compositions will be fabricated and mechanically characterized. Nanoparticles (NP) will be added to the GelMA mixtures to evaluate their effect on the hydrogel’s mechanical properties. Some of the NPs will contain a growth factor so as to study its release rate from the NP. Cotton gauze will be used to simulate wound dressings and GelMA will be used as a medium to deliver antibacterial components onto the cotton. The tensile properties of cotton gauze was first evaluated, GelMA was then added to the cotton gauze to study if it will alter the strength of the cotton. Anti-bacterial tests were also conducted by mixing curcumin, chitosan and silver NP into the GelMA crosslinked on the cotton gauze. Agar diffusion and broth microdilution assays were performed to determine the efficacy of the various additives in combatting bacterial infections. Two strains of bacteria, Escherichia coli and Staphylococcus aureus (to model Gram negative and Gram positive bacteria respectively), were used in the tests. It was found that GelMA hydrogel’s can be significantly improved by varying its fabrication parameters as well as adding NP. GelMA was found to have minimal effect on strengthening cotton scaffolds and that cotton can be loaded with GelMA containing antibacterial components to adequate effect.