The acute effect of phosphorus supplementation on postprandial blood lipid responses in healthy male adults -

Abstract

It is well-known that phosphorus ingestion affects insulin sensitivity (Khattabet al., 2011). Evidence of insulin insensitivity has been previously observed in patients with hypophosphatemia (Defronzo and Lang, 1980). Indeed, phosphate plays an important role in insulin signaling and receptor affinity (Lizcano and Alessi, 2002). Phosphorus is also involved in the metabolism of fats. The enhanced lipogenesis (due to increased hepatic triglyceride production) and the reduced triglycerides clearance (due to inhibited activity of lipoprotein lipase activity) observed in hypertriglyceridemia of hypophosphatemia, were proposed to be the result of insulin resistance (Haglin, 2001). Therefore, the objective of this study is to investigate the acute effects of phosphorus supplementation on postprandial lipid responses in healthy adults. Eight healthy male subjects with normal BMI were recruited to participate in a randomized cross-over study. On each visit, overnight fasted subjects received a high fat meal [330 Kcal; Energy (percent): 69 fat, 28 CHO, 3 protein] and were randomly given 500 mg phosphorus, or 125 mg phosphorus or a placebo pill. Blood samples were collected at baseline (fasting), and after meal ingestion at 60, 120, 180, 240, 300, and 360 minutes. Serum total phosphate, inorganic phosphate, insulin, glucose, triglycerides, apo-B48, NEFA, HDL, LDL, total cholesterol, and plasma apo-B100 were measured. Results were observed as means ± standard error. Data was analyzed by ANOVA test. Higher concentrations of apo-B48 and triglycerides; reduced levels of apo-B100 were observed after the intake of the 500 mg P; but not the 125 mg P intake.However, none of these differences reached statistical significance. Insulin, NEFA, Total cholesterol, glucose, and HDL concentrations were minimally affected by phosphorus supplementation. These findings imply that there was a trend towards higher apo-B48 and TG response that was sustained for longer duration, and a decreased apo-B100 response after the supplementation with 50